ANTIMICROBIAL ACTIVITY OF BARLERIA PRIONITIS ON PATHOGENIC STRAINS
Objectives: The present study was planned to investigate the antimicrobial activity of different part of the plant drug. Therefore, the preliminary successive solvent extraction and chemical test revealed the presence of secondary metabolites in various extracts, and provide us clue for further investigation.
Methods: Different solvents i.e., Chloroform, Acetonitrile, Ethanol, was used for extraction of active secondary metabolites by the Soxhlet method. Percentage of yield4, and antimicrobial activity by gradient plate technique . Statistical analysis has done by standard error by mean (SEM), in which n=3.
Results: The significant antimicrobial activity of leaf, stem and root along with 1.2Â±0.0136 mg/ml to 4Â±0.0128 mg/ml on bacterial strain and 9.4Â±0.0128 mg/ml to 14.5Â±0.0149 mg/ml MIC value with SEM. The highest percentage of yield was observed in Ethanol, chloroform and Acetonitrile i. e from 3.7% to 5.36%. Various numbers of secondary metabolites were extracted from 1 to 4 in Barleria prionitis Linn.
Conclusion: Ethanol has been shown as a best solvent to yield, extracted components and antimicrobial effects prospects while acetonitrile and chloroform were also good solvents to extract secondary metabolite components.Keywords: Barleria prionitis, Secondary metabolites, Antimicrobial activity, Solvents.
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