THE TOXICITY EFFECT OF MONOCROTOPHOS 36% E. C ON THE HAEMATOLOGY, LABEO ROHITA (HAMILTON, 1882)
Objective: Pesticides are stable compounds and they enter the aquatic ecosystem through the agriculture run off. The evaluation of nature and degree of harmful effects produced by the toxic substance in the aquatic organisms are evaluated by toxic tests. The 96 hour LC50 values have generally been found to be satisfactory for the measurement of acute toxicity. The differences in 96 h LC50 of the same toxicant in different fishes may be attributed to individual traits including those of behavior and additional structure such as accessory respiratory organs. The individual characters such as size and weight, sex and biological behavior are important determination for variation in LC50 values.
Methods: Therefore, in this present study is an attempt to study the toxicity of the pesticide with respect to the hematology, biochemical and histology of fish Labeo rohita (Ham). The Monocrotophos affects not only fish but also organisms in the food chain through the process of consumption of one by the other. The pesticide, which enters the body tissues of the fish, affects the physiological activities.
Results: The cytometric measurements of erythrocytes of sublethal exposure showed that there is not much differences from the control. In the control fish, the erythrocytes were oval in shape with elongated nucleus. Fish, exposed to sublethal concentration of Monocrotophos showed abnormal size Reduction in the volume of the cytoplasm of cells and swelling of nuclei were observed in fish exposed to concentration.
In the hematology, the total Red Blood Corpuscle and Haemoglobin content were decreased with the increasing hours of exposure of the monocrotophos 36% EC. The amount of the Mean Corpuscular Haemoglobin (MCH) also was increased. The haemoglobin content directly relationship for RBC content it indicate count leading to anemia as a result of inhibition of erthropoiesis, haemosynthesis and increase in the rate of erythrocyte destruction in haemopoietic organs.
However, the total was total White Blood Corpuscle was increased with the increasing hours of exposure of the Monocrotophos 36% E. C. The constant increasing in the differential count clearly indicates that the pesticide stress certainly stimulate the white blood cells to produce more at all time of exposure. A linear relationship was established with respect to pesticide monocrotophos and total White Blood Corpuscle. The constant increase in the differential count clearly indicates that the pesticide stress certainly stimulate the white blood cells to produce more at all times of exposure.
Conclusion: It has been suggested that the enumeration of differential cell ratio counts provide of useful diagnostic procedure to assess the physiological stress in the fish.Keywords: Labeo rohita, Monocrotophos, Heamatology.
2. Herger W, Jung SJ, Peter H. Acute and prolonged toxicity to aquatic organisms of new and existing chemicals and pesticides. Chemosphere 1995;31:2707-26.
3. Veeraiah. Effect of pesticide on non-target organisms. Residue Rev 2012;76:173-301.
4. Nagaraju, B Venkata, Rathnamma V. Effect of profenofos an organophosphate on protein level in some tissues of fresh water fish labeo rohita (Hamilton). Int J Pharm Sci 2013;5(Suppl 1):276-9.
5. Mhadhbi L, Palanca A, Gharred T, Boumaiza M. Bioaccumulation of metals in tissues of Solea vulgaris from the outer coast and Ria de Vigo, NE Atlantic (Spain). Int J Environ Res Publ Health 2012;6:19-24.
6. Cattaglin WA. Fairchild JF. Potential toxicity of pesticides measured in mid-western streams to aquatic organisms. Water Sci Technol 2002;45:95-103.
7. Tont S. Food source of the oceans: an outline status of potential. Environ Conserv 1977;4:243.
8. Thangnipon W, P Luangpaiboon, W Thangnipon, S Chinobul. Effects of the organophospahate insecticide, monocrotophos. On acetylcholine esterase activity in the tilapia fish (Orecheomosis niloticus) brain. J Neurochem Res 1995;20:515-9.
9. Dubois KP, Geiling EMK. Text book of Toxicology. Oxford University Press. Oxford; 1959. p. 302.
10. Ward GS, Parrish PR. Manual of methods in aquatic environment research. Part 6 Toxicity test FAO. fish tech. pub; 1982. p. 185.
11. Muthukumaravel K, Rajarajan P, Nathiya N, Govindarajan M, Raveendran S. Studies on the histopathology of selected organ of fresh water water fish Labeo rohita exposed to pesticide monocrotophos; 2013.
12. Nisar Shaikh, Yeragi SG. Effect of rogor 30E (organophosphate) on muscle protein in the fresh water fish Lepidocephalecthyes thermalis. J Ecotoxicol Environ Morit 2004;14:233-5.
13. Anees MA. Acute toxicity of four organophosphours insecticides to fresh water teleost Channa punctatus. Park J Zoo 1975;7:135.
14. Arunachalam S, Palanichamy S. Sublethal effects of carboryl on surfacing behavior and food utilization in the air breathing fish Macropods cupanus. Physiol Behav 1982;29:23-7.
15. Baskaran P, Palanichamy S, Balasubramanian MP. Effect of pesticides on protein metabolism in Mystus vittatus. J Ecobiol 1989;1:90-7.
16. Singh NN, VK Das, S Singh. Effect of aldrin on carbohydrate, protein and ionic metabolism of a fresh water catfish Heteropneustes fossilis. J Bulletin of Environ Contam Toxicol 1996;57:204-10.
17. Malla Reddy P, Bashamohideen MD. Toxic impact of fenvalarate on protein metabolism in the branchial tissue of a fish Cyprinus carpio. Curr Sci 1989;57:211-2.
18. Gurusamy K, Ramadoss V. Impact of DDT on oxygen consumption and opercular activity of Lepidocephalichthys thermalis. J Ecotoxicol Environ Monit 2000;10:239-48.
19. Sapna shrivasatava, Sudha Singh, Keerty Shrivastava. Effect of carbaryl on glucose content in the brain of Heteropneustes fossilis J Ecotoxicol Environ Monit 2002;12:205-8.
20. Nisar Shaikh, Yeragi SG. Effect of Rogor 30E (organophosphate) on muscle protein in the fresh water fish Lepidocephalecthyes thermalis. J Ecotoxicol Environ Monit 2004;14:233-5.
21. Visvanathan P, C Maruthanayagam, M Govindaraju. Effect of malathion and endosulfan on biochemical changes in Channa punctatus. J Ecotoxicol Environ Monit 2009;19:251-7.
22. Adhikari S, Sarkar B, Chatterjee A, Mahapatra CT, Ayyappan S. Effects of cypermethrin and carbofuran on certain haematological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton). Ecotoxicol Environ Saf 2004;58:220â€“6.
23. Cazenave J, Wunderlin DA, Hued AC, de los Angeles-Bistoni M. Haematological parameters in a neotropical fish, Corydoras paleatus (Jenyns, 1842) (Pisces, Callichthyidae), captured from pristine and polluted water. Hydrobiology 2005;537:25â€“33.
24. Houston AH. Are the classical hematological variables acceptable indicators of fish health Trans. Am Fish Soc 1997;126:879-93.
25. Sulekha BT. Certain biological aspects on the effect of pesticides on fishes. Ph. D. Thesis. Mahatma Gandhi University, Kerala; 2002.
26. American Public Health Assosciation. Standard Methods of water and wastewater. 18th ed. American water works Association, Water Environment Federation Publication. APHA, Washongton D.C; 1992.
27. Finney DJ. Probit Analysis. Cambridge Univ. Press: London. Folch J, M Lees, SH Sloane-Stanley. A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 1957;226:497-507.
28. Johansen. Effects of aldiocarbon the blood and tissues of a fresh water fish. Bull Environ Contam Toxicol 1970;38:36-41.
29. Fukusima H, a Bailone RL, Weiss LA. Triploidy in the hematology of jundia juvaniles (Siluriformes: Hetapterida) Braz. J Biol 2012;72:147-55.
30. Venkataramana GV, PN Sandhya, PS Murthy. Impact of malathion on the biochemical parameter of gobid fish, Glossogobius giuris (Ham). J Environ Biol 2006;27:119-22.
31. Sudha summarvar K. Haematological investigation on Labeo rohita following chronic exposure to zn and cu; 2012.
32. Patil VK, M David. Hepatotoxic potential of malathion in the freshwater teleost, Labeo rohita (Hamilton). Veteinarski Arch 2007;73:179-88.
33. Binukumari S, J Vasanthi. Studies on the effect of pesticide Dimethoate 30% EC on the lipid content of the fresh water fish labeo rohita; 2013.