• N. Raaman Natural Products and Tissue Culture Laboratory, Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600025, India


H auriculata, Phenols, Flavonoids, DPPH, Nil, Nil, Nil, Reducing power


Objective: The aim of the present investigation was to evaluate the phytochemical constituents, and antioxidant properties of the methanol extract of leaves of Hygrophila auriculata.

Methods: The phytochemicals in the methanol extract of leaves of H. auriculata were determined qualitatively and quantitatively using standard methods. The antioxidant activities were carried out by DPPH free radical scavenging assay, ABTS∙+radical cation scavenging assay OH• radical scavenging assay, NO•radical scavenging assay, Fe2+chelation assay, Fe3+reducing power assay and phosphomolybdenum reduction assay methods.

Results: The methanol extract of leaves of H. auriculata revealed good radical scavenging activities and reducing power activities which were found to increase with the increasing concentration of the extract. The presence of phenols and flavonoids in the methanol extract of leaves of H. auriculata were 342.64 mg/g and 124.4 mg/g, respectively.

Conclusion: The present study revealed that the methanol extract of leaves of H. auriculata showed significant antioxidant activities.


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Chopra RN, Nayer SL, Chopra IC. Glossary of Indian Medicinal plants, National Institute of science and Communication. C. S. I. R Publication: New Delhi: India: 1956. p. 330-2.

Yusuf M, Begum J, Hoque MN, Chowdhury JU. Medicinal plants of Bangladesh, Bangladesh Council Sci India Res 2009. p. 462-3.

Nadkarni AK. Indian material medica. Popular prakashan private limited, Bombay: India: 1978. p. 667-9.

Raaman N. Phytochemical techniques. New India Publishing Agency, New Delhi; 2006. p. 306.

Trease GE, Evans WC. Textbook of Pharmacognosy. 12th Ed. Balliese Tindall and Company Publisher, London; 1983. p. 343-83.

Harbome JB. Phytochemical Methods. Chapman and Hall Ltd, London; 1973. p. 49-188.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999;299:152-78.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;29:1199-200.

Ulyana A, Daniel E, Michel H, Edward J, Kennelly S. Antioxidant activity of browning reaction prepared from glucosamine. Phytother Res 2002;16:63-5.

Delgado-Andrade C, Rufia´n-Henares JA. Morales FJ. Assessing the antioxidant activity of melanoidins from coffee brews by different antioxidant methods. J Agric Food Chem 2005;53:7832–6.

Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical generating systems. Biochemistry 1981;20:6006-12.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide scavenging property of Ginkgo biloba extract EGb 761. Biochem Biophys Res Commun 1994;201:748-55.

Dinis TCP, Madeira VMC, Almeida MLM. Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161-9.

Ravisankar N, Sivaraj C, Seeni S, Joseph J, Raaman N. Antioxidant activites and phytochemical analysis of methanol extract of leaves of Hypericum hookerianum. Int J Pharm Pharm Sci 2014;6:456-60.

Prieto P, Pineda M, Anguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex. Specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-41.

Scott BC, Butler J, Halliwell B, Aruoma OI. Free radical activity of plant extracts. Free Rad Res Commun 1993;19:241â€53.

Kunchandy E, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharmacol 1990;58:237â€40.

Johnston JW, Dussert S, Gale S, Nadarajan J, Harding K, Benson EE. Optimisation of the azinobis-3-ethyl-benzothiazoline-6-sulphonic acid radical scavenging assay for physiological studies of total antioxidant activity in woody plant germplasm. Plant Physiol Biochem 2006;44:193-201.

Hochestein P, Atallah AS. The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer. Mutat Res 1988;202:363-75.

Srikanth G, Babu SM, Kavitha CHN, Roa MEB, Vijaykumar N, Pradeep CH. Studies on in vitro antioxidant activities of Carica papaya aqueous leaf extract. Res J Pharm Biol Chem Sci 2010;1:59-65.

Kappus H. Lipid peroxidations mechanism and biological relevance. In: Aruoma OI, Halliwell B. Eds. Free Radicals and Food Additives. Taylor and Francis: London UK; 1991. p. 59-75.

Moncada A, Palmer RMJ, Higgs EA. Nitric oxide, Physiology, Pathophysiology and pharmacology. Pharmacol Rev 1991;43:109â€42.

Aboul-Enein AM, El Baz FK, El-Baroty GS, Youssef AM, Abd El-Baky HH. Antioxidant activity of algal extracts on lipid peroxidation. J Med Sci 2003;3:87-98.

Gordon MF. The mechanism of antioxidant action in vitro. In: B. J. F. Hudson (Ed.). Food antioxidants. Elsevier Science, London; 1990. p. 1-18.

Xing R, Liu S, Guo Z, Yu H, Wang P, Li C, et al. Relevance of molecular weight of chitosan and its derivatives and their antioxidant activities in vitro. Bioorg Med Chem 2005;13:1573-7.

Dorman HJD, Peltoketo A, Hiltunen R, Tikkanen MJ. Characterization of the antioxidant properties of deodorised aqueous extracts from selected Lamiaceae herbs. Food Chem 2003;83:255-62.

Aderogba MA, Okoh EK, Idowu TO. Evaluation of the antioxidant activity of the secondary metabolites from Pilostigma reticulatum (DC.). Hochst Biol Sci 2005;5:239-42.



How to Cite

Raaman, N. “ANTIOXIDANT ACTIVITES AND PHYTOCHEMICAL ANALYSIS OF METHANOL EXTRACT OF LEAVES OF HYGROPHILA AURICULATA (SCHUMACH) HEINE”. International Journal of Current Pharmaceutical Research, vol. 7, no. 4, Oct. 2015, pp. 100-5,



Original Article(s)