Quantification and determination of antioxidants in Syzygium cumini: Revalance to human health


Amit Gupta

Abstract


Introduction- As per the literature, Syzygium cumini, medicinal plant products i.e. bark, leaves, seed and fruits are used as an alternative medicine to treat various diseases. Inspite of ites medicinal uses, this work was further carried out to determined the protein content including antioxidant (i.e. vitamins C and E) content in aqueous leaves extract of Syzygium cumini in lysed human whole blood.

 Methods- For these studies, we analyzed various antioxidants especially vitamins (C and E) in lysed human whole blood using aqueous leaves extract of Syzygium cumini (10 mg/ml; stock solution). In addition, estimation of protein content through Nanodrop method.  For these studies, capsules of antioxidants {Vitamin C (Celin) and vitamin E (Evion) were used as standard.

 

Results- The results showed that Syzygium cumini significantly increased in Vitamin C production as compared to antioxidant (Vitamin C capsule) control but there is little enhancement in vitamin E production. In addition, two bands of protein (45 and 60 kDa) are observed in aqueous leaves extract of Syzygium cumini.

 

Conclusion- Aqueous leaves extract of Syzygium cumini showed significant enhancement in vitamin C production. The study of this medicinal plant is very important and provide a scientific data of particular importance for the local people using these plant products for a variety of body disorders.


| PDF |

References


REFERENCES

Aruoma O. Free radicals, antioxidants and international nutrition. Asia Pac J Clin Nutr 1999;8:55-63.

Halliwell B. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol 2006;141:312-22.

Green GA. Review: antioxidant supplements do not reduce all-cause mortality in primary or secondary prevention. Evid Based Med 2008;13:177.

Mursu J, Robien K, Harnack LJ, Park K, Jacobs DR, Jr. Dietary supplements and mortality rate in older women: the Iowa Women's Health Study. Arch Intern Med. 2011; 171:

-1633.

Bjelakovic G, Nikolova D, Gluud L, Simonetti R, Gluud C. Antioxidant supplements for prevention of mortality in healthy participants and patients with various diseases.

Cochrane Database of Systematic Reviews. 2012; 3.

Devasagaya TP, Tilak JC, Boloor KK. Free Radicals and Antioxidants in Human Health: Current Status and Future Prospects. J Assoc Physicians India 2004; 52:794-804.

Hardie LJ, Fletcher TC, Secombes CJ. The effect of dietary vitamin C on the immune response of the Atlantic salmon (Salmo salar L.). Aquaculture 1991; 95 (3–4): 201–214.

Gropper SS, Smith JL, Grodd JL. Advanced nutrition and human metabolism. Belmont CA Thomson Wadsworth 2005; 260–275.

Padayatty SJ, Katz A, Wang Y, Eck P, Kwon O, Lee JH, Chen S, Corpe C, Dutta A, Dutta SK, Levine M. Vitamin C as an antioxidant: evaluation of its role in disease prevention. J Am Coll Nutr 2003; 22 (1): 18–35.

Emadi-Konjin P, Verjee Z, Levin AV, Adeli K. Measurement of intracellular vitamin C levels in human lymphocytes by reverse phase high performance liquid chromatography (HPLC). Clinical Biochemistry 2005; 38 (5): 450–456.

Brigelius-Flohe R, Traber MG. Vitamin E: Function and metabolism. FASEB J 1999; 13: 1145-1155.

Zingg JM. Modulation of signal transduction by vitamin E. Mol Aspects Med 2007; 28: 481-506.

Wu D, Liu L, Meydani M, Meydani SN. Effect of vitamin E on prostacyclin (PGI2) and prostaglandin (PG) E2 production by human aorta endothelial cells: mechanism of action. Ann New York Acad Sci 2004; 1031: 425-427.

Heller R, Werner-Felmayer G, Werner ER. Alpha-Tocopherol and endothelial nitric oxide synthesis. Ann New York Acad Sci 2004; 1031: 74-85.

Mahmoud II, Marzouk MS, Moharram FA, El-Gindi MR, Hassan AM. Acylated flavonol glycosides from Eugenia jambolana leaves. Phytochemistry. 2001;58:1239–1244.

Gupta A, Chaphalkar SR. Flow cytometry based assay of formulation from Syzygium cumini in human whole blood and glycosylated red blood cells. Journal of Pharma research 2014; 3 (12): 265 - 270.

Reynertson KA, Basile MJ, Kennelly EJ. Antioxidant potential of seven myrtaceous fruits. Ethnobot Res Appl 2005;3:25–35.

Timbola AK, Szpoganicz B, Branco A, Monache FD, Pizzolatti MG. A new flavonoid from leaves of Eugenia jambolana. Fitoterapia. 2002;73:174–176.

Gupta GS, Sharma DP. Triterpenoid and other constituents of Eugenia jambolana leaves. Phytochemistry. 1974; 13:2013–2014.

Gupta A, Shah AP, Chabukswar AR, Chaphalkar SR. Extraction of proteases from leaves of Mimusops elengi and its immunopharmacological applications. Indo American Journal of Pharmaceutical Sciences 2016; 3(3): 211 – 220.

Gupta A, Jayaprakash, Shinde B. Quantification of protein content and determined its antioxidant activity in Azadirachta indica. Specialty Journal of Biological Sciences 2016; 2(1): 28-39.




About this article

Title

Quantification and determination of antioxidants in Syzygium cumini: Revalance to human health

Date

01-01-2017

Additional Links

Manuscript Submission

Journal

Innovare Journal of Health Sciences
Vol 5 Issue 1 2017 (January-February) Page: 21-24

Online ISSN

2347-5536

Statistics

59 Views | 99 Downloads

Authors & Affiliations

Amit Gupta
Vidya Pratishthan School of Biotechnology
India


Refbacks

  • There are currently no refbacks.