• Bijo Elsy
  • Aijaz Ahmed Khan
  • Veena Maheshwari


Objective: This present study is designed to explore the neuroregenerative effects of d-α-tocopherol on peripheral nerve in both healthy and diabetic
Methods: 24 albino rats were divided into four groups; healthy control, diabetic control, healthy treated, and diabetic treated. Diabetes was induced
by single subcutaneous injection of alloxan (100 mg/kg). Treated groups were administered d-α-tocopherol orally, daily (200 mg/kg) for 3 weeks.
Through a mid-thigh incision, sciatic nerve was crushed with Kocher's forceps, and skin wound was closed with absorbable suture. Sciatic functional
and static indices were used to assess the functional recovery in conjunction with histological, histomorphological, and biochemical analyses at the
end of 3 weeks.
Results: It was noticed that d-α-tocopherol supplementation accelerates functional recovery, enhances the antioxidant levels, and promotes the
regeneration and extracellular matrix reorganization of peripheral nerves.
Conclusion: It is concluded that the d-α-tocopherol appears to be an optimistic option in the management of peripheral nerve crush injury in both
healthy and diabetics.
Keywords: Antioxidant, d-α-tocopherol, Diabetes, Rats, Regeneration, Sciatic nerve.



Sireesha K, Rao SP. Oxidative stress and diabetes: And overview. Asian

J Pharm Clin Res 2015;8:15-9.

Bin-Jaliah I, El-Attar S, Khaleel EF, El-Sayed LA, Haidara MA.

Remedial effects of vitamin e and l-arginine on peripheral neuropathy

in streptozotocin-induced diabetic rats. Am J Pharmacol Toxicol


Negi G, Kumar A, Kaundal RK, Gulati A, Sharma SS. Functional

and biochemical evidence indicating beneficial effect of melatonin

and nicotinamide alone and in combination in experimental diabetic

neuropathy. Neuropharmacology 2010;58(3):585-92.

Merenda A, Bullock R. Clinical treatments for mitochondrial

dysfunctions after brain injury. Curr Opin Crit Care 2006;12(2):90-6.

Lalkovicová M, Danielisová V. Neuroprotection and antioxidants.

Neural Regen Res 2016;11(6):865-74.

Cotter MA, Love A, Watt MJ, Cameron NE, Dines KC. Effects of

natural free radical scavengers on peripheral nerve and neurovascular

function in diabetic rats. Diabetologia 1995;38(11):1285-94.

Cameron NE, Cotter MA, Horrobin DH, Tritschler HJ. Effects of alphalipoic


on neurovascular function in

diabetic rats:




fatty acids. Diabetologia 1998;41(4):390-9.

Sen CK, Khanna S, Roy S. Tocotrienol: The natural vitamin E to defend

the nervous system? Ann N Y Acad Sci 2004;1031:127-42.

Khan AA, Ajmal M, Faizal MP, Elsy B, Faruqi NA. Effects of

vitamin C on regeneration of sciatic nerve crush injuryin adult rats - A

light microscopic study. J Int Acad Res Multidiscip 2015;3:68-77.

Khan AA, Faruqi NA, Ansari MS. Effects of hydrocortisone on the

sciatic nerve crushes injury in adult rat - A light microscopic study. Curr

Neurobiol 2014;5:11-6.

Chalfoun CT, Wirth GA, Evans GR. Tissue engineered nerve constructs:

Where do we stand? J Cell Mol Med 2006;10(2):309-17.

Jiao H, Yao J, Yang Y, Chen X, Lin W, Li Y, et al. Chitosan/

polyglycolic acid nerve grafts for axon regeneration from prolonged

axotomized neurons to chronically denervated segments. Biomaterials


Vleggeert-Lankamp CL. The role of evaluation methods in the

assessment of peripheral nerve regeneration through synthetic

conduits: A systematic review. Laboratory investigation. J Neurosurg


Elsy B, Maheshwari V, Khan AA. Effects of d-α-tocopherol on

progression of reepithelialization, matrix remodeling and appearance

of epidermal appendages in secondary skin wounds of diabetic rats.

J Dermatol Clin Res 2016;4:1081-7.

Bain JR, Mackinnon SE, Hunter DA. Functional evaluation of complete

sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast

Reconstr Surg 1989;83(1):129-38.

Bervar M. Video analysis of standing - An alternative footprint analysis

to assess functional loss following injury to the rat sciatic nerve.

J Neurosci Methods 2000;102(2):109-16.

Sinha AK. Colorimetric assay of catalase. Anal Biochem


Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as

a measure of antioxidant powerâ€: The FRAP assay. Anal Biochem


Hawkins CL, Davies MJ. Generation and propagation of radical

reactions on proteins. Biochim Biophys Acta 2001;1504(2-3):196-219.

Baydas G, Sonkaya E, Tuzcu M, Yasar A, Donder E. Novel role

for gabapentin in neuroprotection of central nervous system

in streptozotocine-induced diabetic rats. Acta Pharmacol Sin


Fan L, Yu Z, Li J, Dang X, Wang K. Schwann-like cells seeded in

acellular nerve grafts improve nerve regeneration. BMC Musculoskelet

Disord 2014;15:165.

Algaidi S. The effect of antioxidants on experimentally induced diabetic

peripheral neuropathy in adult male albino rats. J Am Sci 2011;7:671-7.

Navarro X, Vivó M, Valero-Cabré A. Neural plasticity after peripheral

nerve injury and regeneration. Prog Neurobiol 2007;82(4):163-201.

Sarikcioglu L, Demirel BM, Utuk A. Walking track analysis: An

assessment method for functional recovery after sciatic nerve injury in

the rat. Folia Morphol (Warsz) 2009;68(1):1-7.

Innovare Journal of Health Sciences, Vol 5, Issue 2, 2017, 1-6

Elsy et al.

de Medinaceli L, Freed WJ, Wyatt RJ. An index of the functional

condition of rat sciatic nerve based on measurements made from

walking tracks. Exp Neurol 1982;77(3):634-43.

Malysz T, Ilha J, Nascimento PS, De Angelis K, Schaan BD, Achaval M.

Beneficial effects of treadmill training in experimental diabetic nerve

regeneration. Clinics (Sao Paulo) 2010;65(12):1329-37.

Castro J, Negredo P, Avendaño C. Fiber composition of the rat

sciatic nerve and its modification during regeneration through a sieve

electrode. Brain Res 2008;1190:65-77.

Terada M, Yasuda H, Kikkawa R. Delayed Wallerian degeneration and

increased neurofilament phosphorylation in sciatic nerves of rats with

streptozocin-induced diabetes. J Neurol Sci 1998;155(1):23-30.

Terada M, Yasuda H, Kogawa S, Maeda K, Haneda M, Hidaka H, et al.

Expression and activity of cyclin-dependent kinase 5/p35 in adult rat

peripheral nervous system. J Neurochem 1998;71(6):2600-6.

Yasuda H, Terada M, Maeda K, Kogawa S, Sanada M, Haneda M,

et al. Diabetic neuropathy and nerve regeneration. Prog Neurobiol


Khan AA. Wallerian degeneration in the optic nerve of the rabbit. Cells

Tissues Organs 2004;177(2):104-9.

Breshah MN, Sadakah AA, Eldrieny EA, Saad KA. Functional

and histological evaluation of rat sciatic nerve anastomosis using

cyanoacrylate and fibrin glue. Tanta Dent J 2013;10:67-74.

Sunderland S. Nerves and Vmuz Iqtrirr. 2

ed. Edinburgh: Livingstone;


Tassler PL, Dellon AL, Canoun C. Identification of elastic fibres in the

peripheral nerve. J Hand Surg Br 1994;19(1):48-54.

Pfister BJ, Gordon T, Loverde JR, Kochar AS, Mackinnon SE,

Cullen DK. Biomedical engineering strategies for peripheral nerve

repair: Surgical applications, state of the art, and future challenges. Crit

Rev Biomed Eng 2011;39(2):81-124.

Grinsell D, Keating CP. Peripheral nerve reconstruction after injury:

A review of clinical and experimental therapies. BioMed Res Int


Sanchez EC. Hyperbaric oxygenation in peripheral nerve repair and

regeneration. Neurol Res 2007;29(2):184-98.

Flores AJ, Lavernia CJ, Owens PW. Anatomy and physiology of

peripheral nerve injury and repair. Am J Orthop (Belle Mead NJ)


Agamanolis DP. Neuropathy, An illustrated interactive course for

medical students and residents. Chapter 12, peripheral nerve pathology


Hobson MI. Increased vascularisation enhances axonal regeneration

within an acellular nerve conduit. Ann R Coll Surg Engl


Vasudevan DM, Sreekumari S. Textbook of Biochemistry (For Medical

Students). 4

ed. New Delhi: Jaypee Publication; 2005. p. 340-1.

Jeyashanthi N, Ashok V. Anti-oxidative effect of Cassia auriculata


on streptozotocin induced diabetic rats. Indian J Clin Biochem


Shirpoor A, Ansari MH, Salami S, Pakdel FG, Rasmi Y. Effect of

vitamin E on oxidative stress status in small intestine of diabetic rat.

World J Gastroenterol 2007;13(32):4340-4.

Shelat DY, Acharya SR. Neuroprotective activity of novel cur-ca-thione

and its oxidative stress study. Int J Pharm Pharm Sci 2016;8:167-73.

Brahm KT, Kanti BP, Abidi AB, Ibrahim RS. Markers of oxidative

stress during diabetes mellitus. J Biomark 2013;2013:3.

Nakhaee A, Bokaeian M, Saravani M, Farhangi A, Akbarzadeh A.

Attenuation of oxidative stress in streptozotocin-induced diabetic rats

by Eucalyptus globulus. Indian J Clin Biochem 2009;24(4):419-25.



How to Cite

Elsy, B., Khan, A. A., & Maheshwari, V. (2017). NEUROREGENERATIVE EFFECTS OF D-α-TOCOPHEROL ON THE CRUSHED SCIATIC NERVE OF DIABETIC RATS. Innovare Journal of Health Sciences, 5(2). Retrieved from



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