PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIINFLAMMATORY ACTIVITY OF PERGULARIA DAEMIA (Forsk.)
Keywords:Pergularia daemia, Leaf, Stem, Acetone, Ethyl acetate
Objectives: The in vitro antiinflammatory activity of acetone and ethyl acetate extracts of Pergularia deamia leaf and stem.
Methods: The different parts of extracts were subjected to preliminary phytochemical screening as per the standard protocols. In vitro anti-inflammatory activities were evaluated by red blood cell (RBC) membrane stabilization, protein denaturation, and antiproteinase methods.
Results: Preliminary phytochemical screening revealed that the presence of carbohydrates, phenol, tannins, flavonoids, alkaloids, steroids, and quinines in acetone extracts of plant. In vitro anti-inflammatory activities were tested using different concentrations of the extracts along with standard drug diclofenac sodium. The maximum anti-inflammatory activities were observed in ethyl acetate extracts of P. daemia. As the concentration of the extracts increased antiinflammatory activity also higher.
Conclusion: The plant, therefore, might be considered as a natural source of RBC membrane stabilizers and prevention of protein denaturation, so it is substitute medicine for the management of inflammatory disorder.
Bhaskar VH, Balakrishnan N. Veliparuthi Pergularia deamia Forsk. Chiov. as a phytomedicine a review. Int J Pharm Technol Res 2009;1:1305-13.
Bhagwati U. Utilization of medicinal plants by the rural women of Kullu, Himachal Pradesh. Indian J Tradit Knowl 2003;2:366-70.
Dahanukar SA, Kulkarni AR, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharmacol 2000;32:S81-118.
Basker VH, Balakrishnan N. Veliparuthi Pergulari deamia (Forsk.) (Chiov.) - As a pytomedicine a review. Int J Pharm Technol Res 2009;1:1305-13.
Bahjat FR, Palladino MA, Theodorakis EA, Moldawer LL, Anti TNF α therapies the next generation. Nat Rev Drug Discov 2003;2:736-46.
Deepa M, Renuka D. Potential anti-inflammatory medicinal plants a review. Int J Pharm Sci 2014;6:43-9.
Vane JR, Botting RM. New insights into the mode of action of anti-inflammatory drugs. Inflamm Res 1995;44:1-10.
Pulok K, Peter JM. Evaluation of Herbal Medicinal Products. Houghton: Pharmaceutical Press; 2009. p. 13-22.
Huerta C, Castellsague J, Varas-Lorenzo C, García Rodríguez LA. Nonsteroidal anti-inflammatory drugs and risk of ARF in the general population. Am J Kidney Dis 2002;45:531-9.
Mohammed R, Mohammed U, Surekhasudip D, Patil A. Anti-inflammatory activity of whole plant of Pergularia daemia Linn. Int J Pharm Sci Res 2012;3:258-66.
Sofowara A. Medicinal Plants and Traditional Medicine in Africa. Ladan, Nigeria: Spectrum Books Ltd.; 1993. p. 289.
Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Mungantiwar VJ, Saraf MN. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) Loud wood Oil. Indian J Exp Biol 1999;37:258-61.
Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharmacol Sci 2010;146-55.
Oyedepo OO, Femurewa AJ. Antiprotease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Int J Pharmacong 1995;33:65-9.
Balakumar S, Rajan S, Thirunalasundari T, Jeeva S. Antifungal activity of Aegle marmelos (L.) Correa (Rutaceae) leaf extracts on dermatophytes. Asian Pac J Trop Biomed 2011;1:309-12.
Savithramma N, Linga RM. Screening of medicinal Plants for secondary metabolites. Middle East J Sci Res 2011;8:579-84.
Nyarko AA, Addy ME. Effects of aqueous extracts of Adenia cissampeloides on blood pressure and serum analyte of hypertensive patients. Phytother Res 1990;4:25-8.
Alghazeer R, El-Saltani H. Antioxidant and antimicrobial activities of Cynara scolymus L. rhizomes. Mod Appl Sci 2012;6:17-24.
Senthilkumar M, Gurumoorthy P, Janardhanam K. Antibacterial potential of some plants used by tribals in Maruthamalai hills, Tamil Nadu. Nat Prod Radiance 2011;4:27-34.
Cherian S, Augusti KT. Insulin sparing action of leucopelargonidin derivative isolated from Ficus bengalesis Linn. Indian J Exp Biol 1995;33:608-11.
Luo JJ, Cheung E, Yevich E. Novel terpenoids type quinines isolated from Pycnanthu angolensis of potential utility in the treatment of Type 2 diabetes. J Pharmacol Exp Ther 1999;288:529-34.
Deepikathenmozhi C, Premalakshmi V. Preliminary phytochemical screening and antibacterial activity of Pergularia deamia. Int J Pharma Biosci 2011;2:162-6.
Bhagyasri Y, Lavakumar V, Sree MS, Kumar CK. An overview on anti-inflammatory activity of Indian herbal plants. Int J Res Pharm Nano Sci 2015;4:1-9.
Sangitha C, Priyanka C, Protapaditya D, Sanjib B. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed 2012;2:S178-180.
Chou CT. The anti-inflammatory effect of Tripterygium wilfordii hook F on adjuvant induced paw edema in rats and inflammatory mediators release. Phytother Res 1997;11:152-4.
Murugasan N, Vember S, Damodharan C. Studies on erythrocyte membrane IV. In vitro haemolytic activity of Oleander extract. Toxicol Lett 1981;8:33-8.
Hosseinzadeh H, Younesi HM. Anti-nociceptive and anti-inflammatory effects of Crocus sativus L. Stigma and petal extracts in mice. BMC Pharmacol 2002;2:7.
Rani AA, Mary J, Punitha S, Rema M. Anti-inflammatory activity of flower extracts of Cassla auriculata an in vitro study. Int Res J Pharm Appl Sci 2014;4:57-60.
Arunachalam G, Subramanian N, Pazhani GP, Ravichandran V. Anti-inflammatory activity of methanolic extracts of Eclipta prostrate L. (Astearaceae). Afr J Pharm Pharmacol 2009;3:97-100.
Das SN, Chatterjee S. Long term toxicity study of ART 400. Indian Indigenous Med 1995;16:117-23.
Govindappa M, Sravya SN, Poojashri MN, Sadananda TS, Chandrappa CP. Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extracts and active phytochemical screening of Wedelia trilobata (L.) Hitchc. J Pharmacogn Phytother 2011;3:43-51.