• TAMIL SELVI I Department of Biochemistry, Dharmapuram Gnanambigai Govt Arts College (W), Mayiladuthurai - 609 001, Nagapattinam, Tamil Nadu, India.
  • VIDHYA R Department of Biochemistry, Dharmapuram Gnanambigai Govt Arts College (W), Mayiladuthurai - 609 001, Nagapattinam, Tamil Nadu, India.


Objectives: The in vitro antiinflammatory activity of acetone and ethyl acetate extracts of Pergularia deamia leaf and stem.

Methods: The different parts of extracts were subjected to preliminary phytochemical screening as per the standard protocols. In vitro anti-inflammatory activities were evaluated by red blood cell (RBC) membrane stabilization, protein denaturation, and antiproteinase methods.

Results: Preliminary phytochemical screening revealed that the presence of carbohydrates, phenol, tannins, flavonoids, alkaloids, steroids, and quinines in acetone extracts of plant. In vitro anti-inflammatory activities were tested using different concentrations of the extracts along with standard drug diclofenac sodium. The maximum anti-inflammatory activities were observed in ethyl acetate extracts of P. daemia. As the concentration of the extracts increased antiinflammatory activity also higher.

Conclusion: The plant, therefore, might be considered as a natural source of RBC membrane stabilizers and prevention of protein denaturation, so it is substitute medicine for the management of inflammatory disorder.

Keywords: Pergularia daemia, Leaf, Stem, Acetone, Ethyl acetate.


1. Bhaskar VH, Balakrishnan N. Veliparuthi Pergularia deamia Forsk. Chiov. as a phytomedicine a review. Int J Pharm Technol Res 2009;1:1305-13.
2. Bhagwati U. Utilization of medicinal plants by the rural women of Kullu, Himachal Pradesh. Indian J Tradit Knowl 2003;2:366-70.
3. Dahanukar SA, Kulkarni AR, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharmacol 2000;32:S81-118.
4. Basker VH, Balakrishnan N. Veliparuthi Pergulari deamia (Forsk.) (Chiov.) - As a pytomedicine a review. Int J Pharm Technol Res 2009;1:1305-13.
5. Bahjat FR, Palladino MA, Theodorakis EA, Moldawer LL, Anti TNF ? therapies the next generation. Nat Rev Drug Discov 2003;2:736-46.
6. Deepa M, Renuka D. Potential anti-inflammatory medicinal plants a review. Int J Pharm Sci 2014;6:43-9.
7. Vane JR, Botting RM. New insights into the mode of action of anti-inflammatory drugs. Inflamm Res 1995;44:1-10.
8. Pulok K, Peter JM. Evaluation of Herbal Medicinal Products. Houghton: Pharmaceutical Press; 2009. p. 13-22.
9. Huerta C, Castellsague J, Varas-Lorenzo C, García Rodríguez LA. Nonsteroidal anti-inflammatory drugs and risk of ARF in the general population. Am J Kidney Dis 2002;45:531-9.
10. Mohammed R, Mohammed U, Surekhasudip D, Patil A. Anti-inflammatory activity of whole plant of Pergularia daemia Linn. Int J Pharm Sci Res 2012;3:258-66.
11. Sofowara A. Medicinal Plants and Traditional Medicine in Africa. Ladan, Nigeria: Spectrum Books Ltd.; 1993. p. 289.
12. Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Mungantiwar VJ, Saraf MN. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) Loud wood Oil. Indian J Exp Biol 1999;37:258-61.
13. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharmacol Sci 2010;146-55.
14. Oyedepo OO, Femurewa AJ. Antiprotease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Int J Pharmacong 1995;33:65-9.
15. Balakumar S, Rajan S, Thirunalasundari T, Jeeva S. Antifungal activity of Aegle marmelos (L.) Correa (Rutaceae) leaf extracts on dermatophytes. Asian Pac J Trop Biomed 2011;1:309-12.
16. Savithramma N, Linga RM. Screening of medicinal Plants for secondary metabolites. Middle East J Sci Res 2011;8:579-84.
17. Nyarko AA, Addy ME. Effects of aqueous extracts of Adenia cissampeloides on blood pressure and serum analyte of hypertensive patients. Phytother Res 1990;4:25-8.
18. Alghazeer R, El-Saltani H. Antioxidant and antimicrobial activities of Cynara scolymus L. rhizomes. Mod Appl Sci 2012;6:17-24.
19. Senthilkumar M, Gurumoorthy P, Janardhanam K. Antibacterial potential of some plants used by tribals in Maruthamalai hills, Tamil Nadu. Nat Prod Radiance 2011;4:27-34.
20. Cherian S, Augusti KT. Insulin sparing action of leucopelargonidin derivative isolated from Ficus bengalesis Linn. Indian J Exp Biol 1995;33:608-11.
21. Luo JJ, Cheung E, Yevich E. Novel terpenoids type quinines isolated from Pycnanthu angolensis of potential utility in the treatment of Type 2 diabetes. J Pharmacol Exp Ther 1999;288:529-34.
22. Deepikathenmozhi C, Premalakshmi V. Preliminary phytochemical screening and antibacterial activity of Pergularia deamia. Int J Pharma Biosci 2011;2:162-6.
23. Bhagyasri Y, Lavakumar V, Sree MS, Kumar CK. An overview on anti-inflammatory activity of Indian herbal plants. Int J Res Pharm Nano Sci 2015;4:1-9.
24. Sangitha C, Priyanka C, Protapaditya D, Sanjib B. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed 2012;2:S178-180.
25. Chou CT. The anti-inflammatory effect of Tripterygium wilfordii hook F on adjuvant induced paw edema in rats and inflammatory mediators release. Phytother Res 1997;11:152-4.
26. Murugasan N, Vember S, Damodharan C. Studies on erythrocyte membrane IV. In vitro haemolytic activity of Oleander extract. Toxicol Lett 1981;8:33-8.
27. Hosseinzadeh H, Younesi HM. Anti-nociceptive and anti-inflammatory effects of Crocus sativus L. Stigma and petal extracts in mice. BMC Pharmacol 2002;2:7.
28. Rani AA, Mary J, Punitha S, Rema M. Anti-inflammatory activity of flower extracts of Cassla auriculata an in vitro study. Int Res J Pharm Appl Sci 2014;4:57-60.
29. Arunachalam G, Subramanian N, Pazhani GP, Ravichandran V. Anti-inflammatory activity of methanolic extracts of Eclipta prostrate L. (Astearaceae). Afr J Pharm Pharmacol 2009;3:97-100.
30. Das SN, Chatterjee S. Long term toxicity study of ART 400. Indian Indigenous Med 1995;16:117-23.
31. Govindappa M, Sravya SN, Poojashri MN, Sadananda TS, Chandrappa CP. Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extracts and active phytochemical screening of Wedelia trilobata (L.) Hitchc. J Pharmacogn Phytother 2011;3:43-51.
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