GAS CHROMATOGRAPHY MASS SPECTROMETRY (GC-MS) ANALYSIS AND DOCKING STUDIES OF ANDROGRAPHIS PANICULATA AGAINST DENGUE FEVER


Kanagasabai Somarathinam, Gugan Kothandan, Velmurugan Devadasan

Abstract


Objective: To evaluate gas chromatography- mass spectrometry (GC-MS) analysis and molecular docking studies of ethanol extract of Andrographis paniculata (Burm.f.) against type 2 dengue virus (DEN2).

Methods: The ethanol extract of A. paniculata compounds were identified by GC-MS analysis. These compounds were further analysed for their activities against NS2B/NS3 protease of DEN2 by molecular docking studies.

Results: The nine compounds obtained from the ethanol extract of A. paniculata were characterized and docked. Among these nine compounds, Vor-diazepam,3-[[N-hydroxymethyl]aminocarbonyloxy], Dasycarpidan-1-methanol, acetate (ester), Ethanol, 2-(9, 12-octadecadienyloxy)-, (Z, Z), Gibb-2-ene-1,10-dicarboxylicacid4a,7-dihydroxy-1-methylene-1,4a-lactone,10 methylester, (1a,4aa,4ba,10a) were found to show better interaction energetically and also showed crucial interactions with the active site of NS2B/NS3 protease. Thus A. paniculata is suggested to be a medicinally important plant to cure dengue fever.

Conclusions: From the result, it can be concluded that the crude extract of A. paniculata compounds positively inhibit the activities of NS2B/NSB protease of DEN2 thus by preventing the dengue viral infection. This strategy reflects a logical progression for an early stage drug discovery which can be used to identify new drug candidates. 


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About this article

Title

GAS CHROMATOGRAPHY MASS SPECTROMETRY (GC-MS) ANALYSIS AND DOCKING STUDIES OF ANDROGRAPHIS PANICULATA AGAINST DENGUE FEVER

Date

14-08-2018

Additional Links

Manuscript Submission

Journal

Innovare Journal of Life Sciences
Vol 6 Issue 3 2018 (July-Sept) Page: 15-19

Online ISSN

2321-550X

Authors & Affiliations

Kanagasabai Somarathinam
CAS in Crystallography and Biophysics University of Madras Guindy Campus Chennai-25
India

Gugan Kothandan
CAS in Crystallography and Biophysics University of Madras Guindy Campus Chennai-25
India

Velmurugan Devadasan
CAS in Crystallography and Biophysics University of Madras Guindy Campus Chennai-25
India


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