INHIBITION OF STREPTOCOCCUS MUTANS ADHERENCE AND BIOFILM FORMATION ACTIVITIES FROM MELASTOMA MALABATHRICUM SUBFRACTION


Rohazila Mohamad Hanafiah, Siti Aisyah Abd Ghafar, Wan Ahmad Yaacob, Wan Syaidatul Aqma, Nazlina Ibrahim

Abstract


Objectives: The objectives of the study were to determine antibacterial, anti-adherence, and antibiofilm activities of Melastoma malabathricum stem bark acetone extract (MMSBAE) subfraction against Streptococcus mutans.

Methods: Fraction 9 (F9) from MMSBAE was subfractionated by thin-layer chromatography (TLC) and analyzed for antibacterial activity against S. mutans by TLC-bioautography. Subfraction 12 (SF12) was isolated from F9 followed by determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values.

Results: MIC and MBC values were 10 mg/mL and 160 mg/mL, indicating bacteriostatic property of SF12. Time-kill assay analysis confirmed bacteriostatic property of SF12 against S. mutans. Crystal violet staining and glass surface assays were used to determine anti-adherence and antibiofilm activities. Concentrations produced 50% reduction in anti-adherence and antibiofilm activities were 40 mg/mL and 20 mg/mL, respectively. Scanning electron microscopy was performed to visualize the effect of SF12 on S. mutans biofilm structure. SF12 was found to lyse biofilm formation on treated bacteria indicating powerful anticariogenic potential against S. mutans. Analysis by quantitative real-time polymerase chain reaction revealed SF12 at MIC value downregulated biofilm formation genes such as gbpA, brpA, gtfC, and comDE.

Conclusion: SF12 showed bacteriostatic activities against S. mutans by inhibiting adherence and biofilm activities.


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About this article

Title

INHIBITION OF STREPTOCOCCUS MUTANS ADHERENCE AND BIOFILM FORMATION ACTIVITIES FROM MELASTOMA MALABATHRICUM SUBFRACTION

Date

16-10-2018

Additional Links

Manuscript Submission

Journal

Innovare Journal of Life Sciences
Vol 6 Issue 4, 2018 (Oct - Dec) Page: 1-4

Online ISSN

2321-550X

Authors & Affiliations

Rohazila Mohamad Hanafiah
Department of Basic Sciences and Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Jalan Pandan Utama, Pandan Indah, Kuala Lumpur, Malaysia.
Malaysia

Siti Aisyah Abd Ghafar
Department of Basic Sciences and Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Jalan Pandan Utama, Pandan Indah, Kuala Lumpur, Malaysia.
Malaysia

Wan Ahmad Yaacob
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Malaysia

Wan Syaidatul Aqma
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia.
Malaysia

Nazlina Ibrahim
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia.
Malaysia


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