FREQUENCY OF NUCLEAR ABNORMALITIES IN BUCCAL CELLS OF ROAD CONSTRUCTION WORKERS IN ANAMBRA STATE, NIGERIA
NUCLEAR ABNORMALITIES IN ROAD CONSTRUCTION WORKERS
Keywords:Road construction workers,, Genotoxicity,, Micronuclei,, Asphalt,, Bitumen
Objective: The objective of this study is to assess the frequency of nuclear damage in the buccal cells of road construction workers in Anambra state, Nigeria.
Methods: In this study, the frequency of nuclear abnormalities – cells with micronuclei (CMN), total MN (TMN), binucleate (BN) cells, cells with nuclear bud, and karyorrhectic cells, in exfoliated buccal cells of 33 road construction workers and 33 control subjects consisting of healthy persons was evaluated. Demographic data and exposure characteristics of the participants were obtained using a structured questionnaire. Buccal smears were obtained from both cheeks for each participant. The smears were stained using papanicolaou staining technique. At least 1000 cells in each smear were assessed and scored. The frequency of nuclear abnormalities in both road construction workers and control subjects was then compared.
Results: The results obtained show a significant increase in the frequency of CMN (p=0.005), TMN (p=0.008), and BN (p=0.006) in buccal cells of road construction workers compared with control subjects. Factors such as age, years in occupation, and work days per week, use of protective materials, on-going medication, smoking habit, and alcohol consumption did not significantly affect the frequency of nuclear abnormalities among the road construction workers.
Conclusion: Road construction workers may be exposed to substances capable of inducing nuclear damage in buccal cells.
IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Bitumens and Bitumen Emissions, and Some N-and S-heterocyclic Polycyclic Aromatic Hydrocarbons. Lyon, France: International Agency Research Cancer WHO; 2013.
Khan MR, Sudha S. Evaluation of genotoxicity in automobile mechanics occupationally exposed to polycyclic aromatic hydrocarbons using micronuclei and other nuclear abnormalities. Iran J Cancer Prev 2012;5:87-92.
Çelik A, Yildirim S, Ekinci SY, Taşdelen B. Bio-monitoring for the genotoxic assessment in road construction workers as determined by the buccal micronucleus cytome assay. Ecotoxicol Environ Saf 2013;92:265-70.
Asphalt Institute. The Bitumen Industry-a Global Perspective: Production, Chemistry, Use, Specification and Occupational Exposure. Lexington: Asphalt Institute; 2008.
Haas EJ Willmer D, Cecala AB, Haas EJ, Cecala AB. Compendium of NIOSH Construction Research. Vol. 69. Ministry of Engineering Conference; 2002. p. 105-9.
Sellappa S, Mani B, Keyan KS. Cytogenetic biomonitoring of road paving workers occupationally exposed to polycyclic aromatic hydrocarbons. Asian Pac J Cancer Prev 2011;12:713-7.
van Thienen G, Spee T. Health effects of construction materials and construction products. J Appl Occup Sci 2008;14:2-23.
Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps. Mutat Res 2008;659:93-108.
Banerjee S, Singh NN, Sreedhar G, Mukherjee S. Analysis of the genotoxic effects of mobile phone radiation using buccal micronucleus assay: A comparative evaluation. J Clin Diagn Res 2016;10:ZC82-5.
Ochei JO, Kolhatkar AA. Medical Laboratory Science: Theory and Practice. London: McGraw Hill Education; 2000.
Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: Methods development. Mutat Res 1992;271:69-77.
Kumar M, Prasad UC, Chandolia B, Manjunath SM, Basu S, Verma S, et al. Can feulgen stain be a reliable biomarker over PAP stain for estimation of micronuclei score? J Clin Diagn Res 2016;10:ZC07-11.
Bloching M, Hofmann A, Lautenschläger C, Berghaus A, Grummt T. Exfoliative cytology of normal buccal mucosa to predict the relative risk of cancer in the upper aerodigestive tract using the MN-assay. Oral Oncol 2000;36:550-5.
Sudha S, Kripa SK, Shibily P, Joseph S, Balachandar V. Biomonitoring of genotoxic effects among shielded manual metal arc welders. Asian Pac J Cancer Prev 2011;12:1041-4.
Klöslová Z, Drímal M, Balog K, Koppová K, Dubajová J. The relations between polycyclic aromatic hydrocarbons exposure and 1-OHP levels as a biomarker of the exposure. Cent Eur J Public Health 2016;24:302-7.
Kriech AJ, Schreiner CA, Osborn LV, Riley AJ. Assessing cancer hazards of bitumen emissions a case study for complex petroleum substances. Crit Rev Toxicol 2018;48:121-42.
Torres-Bugarín O, Zavala-Cerna MG, Nava A, Flores-García A, Ramos- Ibarra ML. Potential uses, limitations, and basic procedures of micronuclei and nuclear abnormalities in buccal cells. Dis Markers 2014;2014:956835.
Nagarathna PK, Wesley MJ, Reddy PS, Reena K. Review on genotoxicity, its molecular mechanisms and prevention. Int J Pharm Sci Rev Res 2013;22:236-43.
Arul P. Application of liquid-based cytology preparation in micronucleus assay of exfoliated buccal epithelial cells in road construction workers. Indian J Dent Res 2017;28:413-7.
Gattás GJ, Cardoso Lde A, Medrado-Faria Mde A, Saldanha PH. Frequency of oral mucosa micronuclei in gas station operators after introducing methanol. Occup Med (Lond) 2001;51:107-13.
Thomas P, Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, et al. Buccal micronucleus cytome assay. Nat Protoc 2009;4:825-37.
Diler SB, Ergene S. Nuclear anomalies in the buccal cells of calcite factory workers. Genet Mol Biol 2010;33:374-8.