FREQUENCY OF NUCLEAR ABNORMALITIES IN BUCCAL CELLS OF ROAD CONSTRUCTION WORKERS IN ANAMBRA STATE, NIGERIA

NUCLEAR ABNORMALITIES IN ROAD CONSTRUCTION WORKERS

  • ONWUKWE OKECHUKWU STEVEN Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.
  • ACHUKWU PU Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.
  • OKWUOSA CN Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.
  • AZUBUIKE NC Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.
  • ENIKE CE Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.
  • ONYEMELUKWE AO Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.
  • NNEDU EB Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.
  • IKEGWUONU IC Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Nigeria.

Abstract

Objective: The objective of this study is to assess the frequency of nuclear damage in the buccal cells of road construction workers in Anambra state, Nigeria.


Methods: In this study, the frequency of nuclear abnormalities – cells with micronuclei (CMN), total MN (TMN), binucleate (BN) cells, cells with nuclear bud, and karyorrhectic cells, in exfoliated buccal cells of 33 road construction workers and 33 control subjects consisting of healthy persons was evaluated. Demographic data and exposure characteristics of the participants were obtained using a structured questionnaire. Buccal smears were obtained from both cheeks for each participant. The smears were stained using papanicolaou staining technique. At least 1000 cells in each smear were assessed and scored. The frequency of nuclear abnormalities in both road construction workers and control subjects was then compared.


Results: The results obtained show a significant increase in the frequency of CMN (p=0.005), TMN (p=0.008), and BN (p=0.006) in buccal cells of road construction workers compared with control subjects. Factors such as age, years in occupation, and work days per week, use of protective materials, on-going medication, smoking habit, and alcohol consumption did not significantly affect the frequency of nuclear abnormalities among the road construction workers.


Conclusion: Road construction workers may be exposed to substances capable of inducing nuclear damage in buccal cells.

Keywords: Road construction workers,, Genotoxicity,, Micronuclei,, Asphalt,, Bitumen.

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ONWUKWE OKECHUKWU STEVEN, ACHUKWU PU, OKWUOSA CN, AZUBUIKE NC, ENIKE CE, ONYEMELUKWE AO, NNEDU EB, & IKEGWUONU IC. (2019). FREQUENCY OF NUCLEAR ABNORMALITIES IN BUCCAL CELLS OF ROAD CONSTRUCTION WORKERS IN ANAMBRA STATE, NIGERIA. Innovare Journal of Life Sciences, 1-4. Retrieved from https://innovareacademics.in/journals/index.php/ijls/article/view/34245
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