ISOLATION AND SCREENING OF ANTAGONISTIC ACTINOMYCETES FROM MANGROVE SOIL
Objective: The objective of the present study was to isolate and screen the antagonistic actinomycetes from mangrove soil in Visakhapatnam.
Methods: A total of 30 actinomycetes isolates were isolated by serial dilution plate technique, of these 20 isolates showed activity in primary screening against test pathogens used in this study. The active isolates were morphologically discrete on the basis of spore mass, colour, formation of aerial and substrate mycelia, production of diffusible pigment and biochemical characterization. The active isolates were subjected to shake flask fermentation and the secondary metabolites were extracted with ethyl acetate and screened for their antimicrobial activities against selected bacterial and fungal pathogens by agar well diffusion method.
Results: Out of 20 isolates, 13 isolates exhibited both antibacterial and antifungal activity and 7 isolates showed only antibacterial activity and did not inhibit fungi used in this study. These isolates were identified as Streptomyces species basing on their morphological, physiological and biochemical characters.Conclusion: The results of the present study evidenced that mangrove source may be beneficial for the discovery of novel antibiotics from actinomycetes
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