ISOLATION AND SCREENING OF ANTAGONISTIC ACTINOMYCETES FROM MANGROVE SOIL

Authors

  • K.V.RAGHAVA RAO Andhra University
  • T. RAGHAVA RAO

Abstract

ABSTRACT:

Objective: The objective of the present study was to isolate and screen the antagonistic actinomycetes from mangrove soil in Visakhapatnam.

Methods: A total of 30 actinomycetes isolates were isolated by serial dilution plate technique, of these 20 isolates showed activity in primary screening against test pathogens used in this study. The active isolates were morphologically discrete on the basis of spore mass, colour, formation of aerial and substrate mycelia, production of diffusible pigment and biochemical characterization. The active isolates were subjected to shake flask fermentation and the secondary metabolites were extracted with ethyl acetate and screened for their antimicrobial activities against selected bacterial and fungal pathogens by agar well diffusion method.

Results: Out of 20 isolates, 13 isolates exhibited both antibacterial and antifungal activity and 7 isolates showed only antibacterial activity and did not inhibit fungi used in this study. These isolates were identified as Streptomyces species basing on their morphological, physiological and biochemical characters.

Conclusion: The results of the present study evidenced that mangrove source may be beneficial for the discovery of novel antibiotics from actinomycetes

Author Biography

K.V.RAGHAVA RAO, Andhra University

Department of Biochemistry Research Scholar

References

References

Holguin G, Vazquez P, Bashan Y; The role of sediment microorganisms in the productivity, conservation, and rehabilitation of mangrove ecosystems: an overview. Biol Fertil soils. 2001; 33: 265-278.

Ratna Kala R, Chandrika V; Effect of different media for isolation, growth and maintenance of actinomycetes from mangrove sediments. Indian journal of Marine Science.1993; 22:297-299.

Long H, Xiang W, Zhuang T, Lin P Microorganism resource of Mangrove ecosystems. Chinese journal of ecology. 2005; 24: 696-702.

Lechevalier H.A, Lechevalier M.P; Biology of actinomycetes. Ann. Rev. Microbio.1967; 21: 71-100.

John N Porter; Prevalence and Distribution of Antibiotic-Producing Actinomycetes. Advances in Applied Micro Biology. 1971; 14:73-92.

Jensen PR, Dwight R, Fenical W; Distribution of actinomycetes in near-Shore tropical marine sediments. Appl. Environ. Microbiol. 1991; 57:1102-110.

Lemos ML, Toranzo AE, Barja JL; Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds. Microbiol Ecol.1985; 11:149-163.

Raghava Rao KV, Ravi Kiran CH, Bhaskar Rao D, Madhavi Y, Koteswara Rao P and Raghava Rao T; Antagonistic Activities of Actinobacteria from Mangrove Sediment. International of Pharmacy Journal and Pharmaceutical Sciences. 2012; 4(1): 364-367.

Westley JW, Evans RH, Sello LH, Troupe N, Liu CM, Blount JF. Isolation and Characterization of antibiotic X-14547A, a novel monocarboxylic acid ionophore produced by Streptomyces antibioticus NRRL 8167. Journal of Antibiotics.1979; 32(2): 100-107.

Vinoth Kumari P, Sivaraji A, Madhumitha G, Mary Saral A, Senthil Kumari B; In vitro antibacterial activities of Picrorhiza Kurroa Rhizome extract using Agar Well Diffusion method. International Journal of Current Pharmaceutical Research. 2010; 2(1): 30-33.

Shirling EB, Gottlieb D; Methods for characterization of Streptomyces species, International journal of systematic bacteriology.1966; 16(3): 313-340.

Thomas G Pridham; Color and Streptomycetes: Report of an international workshop on determination of color of Streptomycetes. Applied Microbiology. 1965; 13(1): 43-61.

Selvameenal L, Radhakrishnan M, Balagurunathan R; Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. Indian Journal of Pharma Sci. 2009; 71(5): 499-504.

Sonashia Velho-Pereira, Kamat N M; Antimicrobial Screening of Actinobacteria using a Modified Cross-Streak Method. Indian J Pharm Sci. 2011; 73(2): 223–228.

Sakthi Velayudham, Kasi Murugan; Diversity and Antibacterial Screening of Actinomycetes from Javadi Hill Forest Soil, Tamilnadu, India. Journal of Microbiology Research. 2012; 2(2): 41-46.

Atta MA, Ahmad MS; Antimycin-A antibiotic biosynthesis produced by Streptomyces Sp. AZ-AR-262: taxonomy, fermentation, purification and biological activities. Australian Journal of Basic and Applied Sciences. 2009; 3(1): 126-135.

Published

01-10-2013

How to Cite

RAO, K., & RAO, T. R. (2013). ISOLATION AND SCREENING OF ANTAGONISTIC ACTINOMYCETES FROM MANGROVE SOIL. Innovare Journal of Life Sciences, 1(3), 28–31. Retrieved from https://innovareacademics.in/journals/index.php/ijls/article/view/697

Issue

Section

Articles