• ADUEMA WADIONI Department of Human Physiology, Faculty of Basic Medical Sciences, PAMO, University of Medical Sciences, Port Harcourt, Nigeria.




Ficus thonningii, Elevated plus maze, Anxiety, Mice


Objective: Since prehistoric times, man has relied on herbal plants as a source of medicine in curing various ailments. This study was carried out to assess the antianxiety-like effect of the ethanol extract of wild fig (Ficus thonningii) in Swiss white (CD-1) mice.

Methods: Thirty Swiss mice were randomly divided into three groups (1–3) (n=10). Group 1 (control) was administered normal rat fed and saline, while Group 2 (low dose) and Group 3 (high dose) were administered 10 mg/kg w/w and 20 mg/kg w/w of the plant extract, respectively. The elevated plus maze was used to assess anxiety-related behaviors.

Results: Data were analyzed using the statistical tools (one-way ANOVA and student t-test). Results revealed anxiety-like behavior in the low- and high-dose-treated mice when compared to the control.

Conclusion: Therefore, administration of F. thonningii reduced an anxiety-like behavior in mice.

Author Biography

ADUEMA WADIONI, Department of Human Physiology, Faculty of Basic Medical Sciences, PAMO, University of Medical Sciences, Port Harcourt, Nigeria.

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Ajayi AO. Antimicrobial nature and use of some medicinal plants in Nigeria. Afr J Biotech 2008;7:595-9.

Prelude JL. Medicinal Plants Database. http://www.metafro.be/prelude/ view_plant?pi=06040.2011.

Schmidt E, Lutter M, McCleland W. Trees and shrubs of Mpumalanga and Kruger National Park. South Africa: Jacana; 2002. p. 80.

Njoronge GN, Kibunga JW. Herbal medicine acceptance, sources and utilization for diarrhea management in a cosmopolitan urban area (Thika, Kenya). Afr J Ecol 2007;45:65-70.

Cousins D, Huffman MA. Medicinal properties in the diets of gorillas: An ethno-pharmacological evaluation. Afr Study Monogr 2002; 23:65 89.

Igoli JO, Tor-Anyin TA, Usman SS, Oluma HO, Igoli NP. Folk medicines of the Beneu valley of Nigeria. In: Singh VK, Govil JN, Hashim S, Sing G, editors. Recent Progress in Medicinal Plants, Ethnomedicine and Pharmacognosy. Raleigh, NC: Science Technology Publishers; 2002. p. 327-38.

Alawa JP, Jokthan GE, Akut K. Ethnoveterinary medical practice for ruminants in the subhumid zone of northern Nigeria. Prev Vet Med 2002;54:79-90.

Arbonier MA. Trees, Shrubs and Lianas of West African Dry Zones. 2nd ed. Netherlands: Margraf Publishers; 2004. p. 412.

Mali RG, Mehta AA. A review on anthelmintic plants. Nat Prod Rad 2007; 7:466-75.

Gelfand M, Mavi S, Drummond RB, Ndemera B. The Traditional Medicinal Practitioner. His Principles of Practice and Pharmacopoeia. Gweru, Zimbabwe: Mambo Press; 1985. p. 411.

Tegbe TS, Adeyinka IA, Baye KD, Alawa JP. Evaluation of feeding graded levels of dried and milled Ficus thonningii leaves on growth performance, carcass characteristics and organ of weaner rabbits. Pak J Nutr 2006;5:548-50.

Byrne JH. Plasticity: New concepts, new challenges. In: Roediger HL, Dudai Y, Fitzpatrick SM, editors. Science of Memory: Concepts. New York: Oxford University Press; 2007. p. 77-82.

Mathieu G, Meissa D. Traditional leafy vegetables in Senegal: Diversity and medicinal uses. Afr J Tradit Complement Altern Med 2007; 4:469 75.

Wondimu T, Asfaw Z, Kelbessa E. Ethnobotanical study of medicinal plants around ‘Dheeraa’ town, Arsi Zone, Ethiopia. J Ethnopharmacol 2007;112:152-61.

Lister RG. Ethologically-based animal models of anxiety disorders. Pharmacol Ther 1990;46:321-40.

John S. Schematic Drawing of the Elevated plus Maze (EPM) Experiment for Behavior Tests with Rats. http://www.ed.wikipedia. org.2010.

Walf AA, Frye CA. The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat Protoc 2007;2:322-8.

He LH, Shi HM, Liu TT, Xu YC, Ye KP, Wang S, et al. Effects of extremely low frequency magnetic field on anxiety level and spatial memory of adult rats. Chin Med J (Engl) 2011;124:3362-6.

Kochenborger L, Levone BR, da Silva ES, Taschetto AP, Terenzi MG, Paschoalini MA, et al. The microinjection of a cannabinoid agonist into the accumbens shell induces anxiogenesis in the elevated plus-maze. Pharmacol Biochem Behav 2014;124:160-6.

Skelly MJ, Weiner JL. Chronic treatment with prazosin or duloxetine lessens concurrent anxiety-like behavior and alcohol intake: Evidence of disrupted noradrenergic signaling in anxiety-related alcohol use. Brain Behav 2014;4:468-83.

Pellow S, Chopin P, File SE, Briley M. Validation of open: Closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985;14:149-67.

Puurunen K. The Effects of Pharmacotherapy and Training of Functional Recovery after Global and Cerebral Ischemia in Rats. Series of Reports, No 58. Kuopio: Department of Neuroscience and Neurology, University of Kuopio; 2001. p. 40.

Popovic-Milenkovic MT, Tomovic MT, Brankovic SR, Ljujic BT, Jankovic SM. Antioxidant and anxiolytic activities of Crataegus nigra Wald. Et kit. Berries. Acta Pol Pharm 2014;71:279-85.

Charkravarthi KK, Ramakrishna A, Nayaran RS. Effects of Glycyrrhiza glarbra root extract on learning and memory in wistar albino rats. Inter J Biol Med Res 2012;3:2059-64.

Komaki A, Hoseini F, Shahidi S, Baharlouei N. Study of the effect of extract of Thymus vulgaris on anxiety in male rats. J Tradit Complement Med 2016;6:257-61.

Suprila R, Himani A. CNS depression, sedative and anxiolytic activity of ethanol exract of fruit of piper Chiba revealed after neuropharmacological screening. Int J Pharm Pharm Sci 2014;6:186-9.



How to Cite

WADIONI, A. (2019). EXPERIMENTAL STUDY ON SWISS WHITE MICE TO EVALUATE THE ANTIANXIETY EFFECT OF COMMON WILD FIG EXTRACT (FICUS THONNINGII). Innovare Journal of Medical Sciences, 7(1), 1–5. https://doi.org/10.22159/ijms.2019.v7i1.29357



Original Article(s)