PREVALENCE OF TSST PRODUCING COAGULASE-NEGATIVE STAPHYLOCOCCUS AUREUS IN WOUND SAMPLES AND CHARACTERIZATION OF MRSA AGAINST TEA EXTRACT
Keywords:Wound samples, β-lactamase, Methicillin-resistant Staphylococcus aureus, Coagulase negative, Toxic shock syndrome toxin, Tea extract
Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is a potential pathogen for hospital-acquired infections. This study was conducted to determine the prevalence of MRSA using tea extract.
Methods: All S. aureus isolates obtained from wound samples were studied for antibiotic resistance pattern using 23 different antibiotics. Based on coagulase negative, S. aureus isolates were identified for toxic shock syndrome toxin (TSST) gene and analyzed using PCR method. The antibacterial activities of tea extract were tested against MRSA using agar well-diffusion method.
Results: A total of 100 wound samples were collected from hospital, where 75% of samples showed presence of S. aureus. About 100% resistance to cefoperazone, ampicillin, penicillin, rifampicin, novobiocin, and vancomycin antibiotics was observed. The isolates showed less resistance <50% toward chloramphenicol (30%), ciprofloxacin (25%), gentamycin (52%), amikacin (38%), and imipenem (33%). Twenty-five isolates were selected for MRSA characterization based on multiple drug resistance pattern. Coagulase-negative S. aureus isolates showed presence of TSST gene. Tea extract (2%) showed effective antibacterial activity against MRSA strains.
Conclusion: The study showed the presence of MRSA at higher level and suggesting to out further epidemiological study on such infections. However, cost-effective and easily available tea extract was found to be the best antimicrobial agent for preventing such bacterial infection and to reduce the risk of emerging resistance.
Shanmugam J. The prevalence, antibiogram and characterisation of Staphylococcus aureus including MRSA among the healthy staff, medical students and patients from Sri Manakula Vinayagar medical college and hospital (SMVMCH), Puducherry. JIACM 2009;4:1.
Kaplan SL. Treatment of community-associated methicillin-resistant Staphylococcus aureus infections. Pediatr Infect Dis J 2005;24:457-8.
Ponraj M, Rubeni V, Zambare V. Prevalence and identification of toxic shock syndrome toxin producing wound isolates of Staphylococcus aureus from Namakkal district of Tamil Nadu. Biochem Indian J 2011;5:96.
Tiwari RP, Bharti SK, Kaur HD, Dikshit RP, Hoondal GS. Synergistic antimicrobial activity of tea and antibiotics. Indian J Med Res 2005;122:80-4.
Gramza A, Pawlak LK, Korczak J, Wasowicz E, Rudzinska M. Tea extracts as free radical scavengers. Pol J Env Stud 2005;14:861-7.
Hamid AA, Aiyelaagbe OO, Usman LA, Ameen OM, Lawal A. Antioxidants: Its medicinal and pharmacological applications. Afr J Pure Appl Chem 2010;4:142-51.
Chakraborty D, Chakraborti S. Bioassay-guided isolation and identification of antibacterial and antifungal component from methanolic extract of green tea leaves (Camellia sinensis). Res J Phytochem 2010;4:78-86.
Akiyama H, Fujji K, Yamasaki O, Oono T, Iwatsuki K. Antibacterial action of several tannins against S. aureus. J Antimicrob Chemother 2001;48:487-91.
Mathur S, Kaner CT, Atoom AM. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol 2006;42:872-4.
Ghenghesh KS, Ghodban AE, Marialigeti K, Esahli H, Tawil A. PCR detection of toxic shock syndrome toxin of Staphylococcus aureus from Tripoli, Libya. J Med Microbiol 2006;55:179-82.
Chitnis D, Verma S, Joshi S, Chitnis V, Hemwani N. Growing problem of methicillin resistant staphylococci-Indian scenario. Indian J Med Sci 2000;54:535-40.
Joshi S, Ray P, Manchanda V, Bajaj J, Chitnis DS, Gautam V, et al. Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence and susceptibility pattern. Indian J Med Res 2013;137:363.
Geary C, Jordens JZ, Richardson JF, Hawcpoft DM, Mitchell CJ. Epidemiological typing of coagulase negative staphylococci from nosocomial infection. J Med Microbiol 1997;4:195-203.
Tsen HY, Yu GK, Wang KC, Wang SJ, Chang MY, Lin LY. Comparison of the enterotoxigenic types, toxic shock syndrome toxin 1 (TSST-1) strains and antibiotic susceptibilities for enterotoxigenic Staphylococcus aureus strains isolated from food and clinical samples. Food Microbiol 1998;15:33-41.
Mbata TI. Preliminary studies of the antibacterial activities of processed Kenyan and Nigerian tea. Internet J Microbiol 2006;2:1.
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