Int J Pharm Pharm Sci, Vol 9, Issue 10, 205-210Original Article


SCREENING OF TOTAL PHENOL AND FLAVONOID CONTENT IN DIFFERENT CYTOTYPES OF TWO SPECIES OF ACHYRANTHESLINN. FROM WESTERN HIMALAYA,INDIA

MANJIT INDER SINGH SAGGOO1, LOVLEEN2

1Department of Botany, Punjabi University, Patiala 147002, Punjab, India, 2Department of Botany, DAV College, Jalandhar 144008, Punjab, India
Email: ms.lovleen7@gmail.com

Received: 07 May 2017 Revised and Accepted: 31 Aug 2017


ABSTRACT

Objective: Genus AchyranthesLinn. belonging to family Amaranthaceae consists of six species. The present study was undertaken to screen the phenolic components in the different cytotypes of two species of Genus AchyranthesLinn. growing in western Himalaya, India.

Methods: Methanol extract of leaves was used to determine the total phenol and flavonoid contents in different cytotypes of A. aspera Linn. and A. bidentataBlume by spectrophotometric method. Total phenol content was expressed as mg gallic acid g-1phenol and total flavonoid content as mg quercetin g-1flavonoid using the standard curves. Further, gallic acid content in methanol extracts of leaves was determined by RP-HPLC method using C-18 column, employing 0.01% (v/v) orthophosphoric acid: acetonitrile (98:2 v/v) as mobile phase at a flow rate of 1 ml/min with ultraviolet (UV) detection at 272nm.

Results: Hexaploid plants of A. aspera Linn. possess the higher amount of phenol (9.16±0.84 mg/g) and flavonoid (78.36±1.63mg/g) constituents in the methanol extract of leaves as compared to its dodecaploid counterparts (7.86±0.08mg/g and 70.20±1.81mg/g respectively). Similarly, phenol and flavonoid contentis found to be more in the methanol extract of leaves of hexaploid plants of A. bidentataBlume (11.93±0.59mg/g and 115.92±1.32mg/g respectively) as compared to its dodecaploid counterparts (9.46±0.75mg/g and 107.76±0.94mg/grespectively). Further, RP-HPLC analysis of gallic acid revealed that higher amount of gallic acid is present in dodecaploid plants of A. aspera Linn. (1.04±0.02 mg/g) and A. bidentataBlume (1.34±0.03 mg/g) as compared to hexaploid counterparts (1.01±0.01mg/g and 1.22±0.05mg/g respectively).

Conclusion: The present investigation revealed that A. aspera Linn. and A. bidentataBlumeplants show immense intraspecific variability in their phenolic components. Hence there is need to evaluate germplasm to select superior genotype for medicinal and conservation purpose.

Keywords: AchyranthesLinn, Cytotypes, Total phenols,Total flavonoids, Western Himalaya


INTRODUCTION

Achyranthes is a genus of about six species in tropical and subtropical regions, mostly in Africa and Asia [1]. Out of three species of the genus available in India, A.asperaLinn. is distributed throughout India and commonly found on hillsides, waste places, roadsides and riverbanks up to the ranges of 2300m while A. bidentataBlume grows in temperate and subtropical Himalaya from Kishtwar to Sikkim, Khasia Hills, Bihar, Konkan, Nilgiris and Travancore at an elevations of 1200 m-2400m.

Traditionally, A. asperaLinn.is used in asthma, cough, oedema, dropsy, piles, boils, eruptions of skin, pneumonia, snake bites and night blindness. It is pungent, antiphlegmatic, antiperiodic, diuretic, purgative and laxative [2]. Ash of the plant is applied externally to ulcers and warts. The crushed leaves rubbed on aching back cure strained back [3]. A fresh piece of root is used as tooth brush. A paste of the roots in water is used in ophthalmia and opacities of the cornea. A paste of fresh leaves is used for allaying pain from thebite of wasps [4]. Inhaling the fumes of A. asperamixed with Smilax ovalifoliaroots is suggested to improve appetite and to cure various types of gastric disorders [5]. The plant is useful in liver complaints, rheumatism, scabies and other skin diseases.

As an important medicinal herb, the plant of A. bidentataBlume is used againstwhooping cough, abdominal pain,backache, trauma, pain and difficulty in movement of knees, blisters in the mouth and cholera, scorpion sting and malarial fever. The plant is diuretic and astringent [6-8].

Further, aperusal of the literature revealed the occurrence of different cytotypes among these species. Therefore, the present investigation has been taken up to evaluate these species with immense medicinal importance for their bioactive contents depending upon their different ploidy levels. The present study is the first attempt to carry out the investigation of total phenol,total flavonoid and gallic acid content in different cytotypes of A. aspera Linn. and A. bidentataBlume.

MATERIALS AND METHODS

Collection of plant material

The healthy plants of A. aspera Linn. and A. bidentataBlume were collected from different localities in western Himalaya of India (table 1). Identification of the specimens was done at Botanical Survey of India, Dehra Dun, India. Voucher specimens have been deposited in the Herbarium, Department of Botany, Punjabi University, Patiala (PUN).

Table1: Chromosome number, ploidy,locality with altitude and accession number (PUN) of different cytotypes of Achyranthesaspera Linn and AchyranthesbidentataBlume from western Himalaya, India

Taxa Chromosome number(2n) Ploidy Locality with altitude (m) Accession number (PUN)
A.aspera Linn. 42 Hexaploid Tathal (Himachal Pradesh), 1100m 59410
A.aspera Linn. 84 Dodecaploid Dheera (Himachal Pradesh), 500m 59411
A.bidentataBlume 42 Hexaploid Dharamkot (Himachal Pradesh), 2100m 58572
A.bidentataBlume 84 Dodecaploid Rehlu (Himachal Pradesh), 950m 58575

Chemicals and reagents

Acetic acid (Fisher Scientific), chloroform (Merck), ethanol (Changshu Yangyuan Chem), acetocarmine (BDH), Folin-Ciocalteu reagent (Loba Chemie), anhydrous Na2CO3 (S D Fine Chem),NaNO2 (Sarabhai M Chemicals), AlCl3 (S D Fine Chem) and NaOH (Loba Chemie) used were of analytic grade. Methanol (Merck), gallic acid (HiMedia), quercetin (Sigma-Aldrich),orthophosphoric acid (Loba Chemie) and acetonitrile (Merck) used were of HPLC grade.

Meiotic analysis

For meiotic analysis, the young floral buds of suitable size were fixed in Carnoy’s fixative (6 alcohol: 3 chloroform: 1 acetic acid v/v/v) for 24 h and then preserved in 70% alcohol at 4˚C until use. For the cytological studies, anthers were squashed in 2% acetocarmine. A number of slides were carefully examined for chromosome counts. Photomicrographs of chromosome counts were made from freshly prepared slides using 80i Eclipse Microscope.

Extract preparation

The leaves of the selected plants were washed thoroughly and air dried in shade at room temperature. The dried leaves were ground into acoarse powder with a mechanical grinder and stored inair tight containers at room temperature for further use. 1g powder of each cytotype was suspended in 50 ml methanol for 48h at room temperature with occasional stirring. After 48h, methanol extract was filtered with Whatman no. 1 filter paper.The filtrate was saved and allowed to dry at 37˚C in an incubator for complete evaporation of methanol. The whole process was repeated three times and finally blackish-green coloured, concentrated leaf extract was obtained. Dried extract was weighed and dissolved in methanol to make afinal concentration of 100 mg/ml of extract and stored in therefrigeratoruntil further use.

Determination of total phenols

Total phenol contentdetermination was performed by using a modified Folin-Ciocalteu reagent method [9]. Folin-Ciocalteu reagent was diluted to 10 fold with double distilled water. 0.1ml of methanol extract of leaves was mixed with 7.9ml of double distilled water. Then, 0.5 ml of diluted Folin Ciocalteu reagent was added and incubated for 3 min at room temperature. After that, 1.5 ml of 20% of anhydrous Na2CO3 (w/v) was added and mixed thoroughly. The mixture was incubated at room temperature for 2 h. The absorbance of the sample was recorded at 765 nm using spectrophotometer (Shimadzu, UVmini-1240 UV-VIS Spectrophotometer) against ablank containing double distilled water instead of leaf extract.The experiment was conducted in triplicate. The total phenol content was determined as mg gallic acid g-1phenol using the standard curve.

Determination of total flavonoids

Total flavonoid content was determined by themethod proposed by Kim et al. [10]with minor modifications. 1ml of methanol extract of leaves was mixed with 4ml of double distilled water. Then, 0.3ml of 5% NaNO2 (w/v) was added and incubated for 5 min at room temperature. Later, 0.3ml of 10% AlCl3 (w/v), 2ml of 1M NaOH and 2.4ml of double distilled water was added. The mixture was incubated up to 30 min at room temperature and absorbance was measured at 510 nm using spectrophotometer (Shimadzu, UVmini-1240 UV-VIS Spectrophotometer). The experiment was performed in triplicate. The total flavonoid content was determined as mg quercetin g-1flavonoid using the standard curve.

RP-HPLC conditions

Reverse phase-high performance liquid chromatography (RP-HPLC) was used for the detection of gallic acid.RP-HPLC was performed by modifying the method proposed byRakeshet al.[11].HPLC (Shimadzu) C-18 column with dimensions 250 mm x 4.6mm and particle size 5μmwas used. The column temperature was maintained at 30 ˚C. Mobile phase used was 0.01% (v/v) orthophosphoric acid: acetonitrile (98:2 v/v) with a flow rate of 1 ml/min. The injection volume was 20 μl and detecting wavelength was 272nm. The mobile phase was filtered through a0.45μm membrane filter.

RESULTS AND DISCUSSION

Chromosome number

The meiotic analysis was carried out on different populations of A. aspera and A. bidentata from western Himalaya, India.The existence of two different chromosome counts of 2n=42, 84 (fig.1) was observed in both the studied species of Achyranthes.The species are hexaploid (2n=42) and dodecaploid (2n=84) based on base number x=7[12]. The present chromosome counts are in conformity with the earlier reports from India and elsewhere [13-38]. However, varied chromosome reports have also been reported earlier for A. asperai.e. 2n=14, 36, 48 and 96[39-41]and A. bidentatai.e. 2n=34, 35, 36, 38 [36, 42].

Fig.1:A-B: Achyranthes asperaLinn.:A. A PMC at metaphase-I showing 21 bivalents,B. A PMC at metaphase-I showing 42 bivalents; C-D: Achyranthes bidentata Blume: A PMC at diakinesis stage showing 21 bivalents,b. A PMC at metaphase-II showing 42:42 distribution of chromosomes. Scale bar=10μm

Phenol and flavonoid estimation

The amount of phenol and flavonoid contents in leaf extracts of A. aspera and A. bidentatawereestimated by spectrophotometric method. Gallic acid was used as a standard compound and total phenoliccontent was expressed as mg gallic acid g−1 usingthe equation based on thecalibration curve (fig. 2): y=0.0007x, R2=0.9946, where y is an absorbanceand x is mg gallic acid g−1. The total flavonoid contentwas expressed as mg quercetin g−1 using the straight lineequation based on thecalibration curve of quercetin standard(fig. 3):y=0.0006x, R2=0.9865, where y is an absorbance and x is the mgquercetin g−1.

Total phenol and flavonoid contents of hexaploid cytotypes of both the species of Achyranthesare higher as compared to dodecaploid cytotypes of respective species (table 2).

Fig. 2: Calibration curve for gallic acid

Fig. 3: Calibration curve for quercetin

Table 2:Total phenol and flavonoid content in leaf extract of hexaploid and dodecaploid cytotypes of A. aspera Linn. and A. bidentataBlume

Species Cytotypes Total phenol content (mg/g) Total flavonoidcontent (mg/g)
A. aspera Linn. Hexaploid 9.16±0.84 78.36±1.63
Dodecaploid 7.86±0.08 70.20±1.81
A. bidentata Blume Hexaploid 11.93±0.59 115.92±1.32
Dodecaploid 9.46±0.75 107.76±0.94

Data expressed as mean±SD [n=3]

Phenolic compounds,broadly distributed in the plant kingdom, are a major class of bioactive components. Phenols and flavonoids have drawn increasing attention due to their marked effects in the prevention of various diseases as these possess diverse biological activities,for instance, antioxidant [43], antitumor [44], antiulcer [45],anti-inflammatory [43, 46] and antidepressant activities [47, 48].

Earlier, different leaf extracts of Achyranthes have been evaluated for different biological activities includingantimicrobialactivity [49, 50],anti-inflammatory activity[51], wound healing activity [52], anti-depressant effect [53], cancer chemo preventive activity[54], antinociceptive activity [55], analgesic activity[56], antifertility activities [57], anti-oxidant activity [58] and diuretic activity [59].

Screening of different phytochemical components from leaf extract has also been done earlier [50, 60-62]. However, no report till date is available on the characterization of phenolic constituents in the leaves of A. asperaand A. bidentata having different ploidy level. The present attempt has been carried out to find out the variation in a number of phenolic constituents with variation in ploidy of the plant.In the present investigation, it has been observed that the plants of Achyranthes aspera and Achyranthesbidentatawith the lower ploidy level i.e. hexaploids possess higher amount phenol and flavonoid constituents in the methanol extract of leaves as compared to its dodecaploid counterparts.

Further, RP-HPLC analysis was performed with the purpose of identifying and quantifying one of the phenolic compound i.e. gallic acid. Identification was performed by comparison of their retention’s time and UV absorption spectrum with those of the standards.Five-point calibration curve of astandard sample of gallic acid is given in fig. 4. Fig. 5 shows the chromatograms obtained from theRP-HPLC separation of gallic acid with methanol extracts of different studied cytotypes of A. asperaand A. bidentata. Results revealed a considerable phytochemical (gallic acid) variation in the studied cytotypes of A. asperaand A. bidentata(table 3). Higher concentration of gallic acid is present in dodecaploid plants of A. aspera and A. bidentata as compared to hexaploid counterparts.

Fig.4: Calibration curve for gallic acid

Fig.5: RP-HPLC chromatograms of methanol extracts of leaves of A.Hexaploid cytotype of Achyranthes asperaLinn., B. Dodecaploid cytotype of Achyranthes asperaLinn., C. Hexaploid cytotype of Achyranthes bidentataBlume, D. Dodecaploid cytotype of Achyranthes bidentataBlume. Chromatograms show gallic acid

Table 3: Gallic acid content in the leaf extract of hexaploid and dodecaploid cytotypes of A. aspera Linn. and A. bidentataBlume

Species Cytotypes Gallic acid (mg/g)
A. aspera Linn. Hexaploid 1.01±0.01
Dodecaploid 1.04±0.02
A. bidentataBlume Hexaploid 1.22±0.05
Dodecaploid 1.34±0.03

Data expressed as mean±SD [n=3]

Gallic acid is a poly phenolic compound and chemically known as 3, 4, 5-Trihydroxybenzoic acid. It is extensively used as adietary herbal supplement as occurs in many plant materials in the form of esters, free acids, catechin derivatives and hydrolysable tannins. Gallic acid is evaluated for various biological activities including antioxidant, antimicrobial, anticancer, cardioprotective and neuroprotective [63-67].In addition, no report till date is reported for the determination of gallic acid in different cytotypes of A. asperaand A. bidentata.

CONCLUSION

Present investigation revealed that A. aspera Linn. and A. bidentataBlumeplants show significant intraspecific variability in their total phenol,total flavonoid and gallic acid contentswhich may be accredited to thegenotype of these species. The variation observed in presently studiedbioactive constituents could be considered as an imperative factor for herbal formulation as theefficiency of the herbal remedy may be subjective to the type of genotype used for its preparation. Additionally, it recommends that there is arequirement for the selection of superior genotypes of these species of Achyranthes before recommending the same for therapeutic purpose and conservation.

ACKNOWLEDGMENT

The authors are grateful to the University Grants Commission, New Delhi for providing facilities under the DRS SAP III and ASISTprogramme. Financial assistance to one of us (Lovleen) by Department of Science and Technology, Government of India, New Delhi, under WOS-A is gratefully acknowledged. Thanks are also due to the Head, Department of Botany, Punjabi University, Patiala for necessary laboratory facilities.

AUTHORS CONTRIBUTION

Saggoo MIS provided guidance and critical review. Lovleen researched and wrote this manuscript. Both authors read and approved the final version of this study.

CONFLICT OF INTERESTS

There is no conflict of interest among the authors

REFERENCES

  1. Harwood RK, Palmer J. Amaranthaceae. In: Short PS, Cowie ID. editors. Flora of the Darwin region. Vol. 1. Australia: Northern Territory Herbarium, Department of Natural Resources, Environment, the Arts, and Sport; 2011. p. 1-23.

  2. Nadkarni KM. Indian materia medica. Vol. I. Bombay Popular Prakashan; 2009. p. 21.

  3. Singh VK, Ali ZA, Zaidi STH, Siddiqui MK. Ethnomedicinal uses of plants from gonda district forests of Uttar Pradesh, India. Fitoterapia 1996;67:129-39.

  4. Gupta RK. Medicinal and aromatic plants. CBS publishers and distributors; 2010. p.190.

  5. Bhattarai NK. Folk herbal remedies of sindhupalchok district, central Nepal. Fitoterapia1992;63:145-55.

  6. Guha Bakashi DN, Sensarma P, Pal DC. A lexicon of medicinal plants in India. Vol. I. Calcutta (India): Naya Prokash (New Sarada Press);1999.

  7. Dhiman AK. Banaras ayurveda series 2-medicinal plants of Uttranchal state. Varanasi (India): Chowkhamba Press; 2004.

  8. Pullaiah T. Encyclopedia of world medicinal plants. Vol. I. New Delhi (India): Regency Publications; 2006.

  9. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.

  10. Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem 2003;51:6509-15.

  11. Rakesh SU, Patil PR, Salunkhe VR, Dhabale PN, Burade KB, Bhise SB. Quantitative estimation of gallic acid from hydroalcoholic extract of dried flowers of Nymphaea stellatawilld by RP-HPLC. Asian J Chem 2010;22:5919-24.

  12. Darlington CD, Wyile AP. Chromosome atlas of flowering plants. London: George Allen and Unwin Ltd; 1955.

  13. Sugiura T. A list of chromosome numbers in angiospermous plants. Bot Mag1931;42:504-6.

  14. Sugiura T. Studies on the chromosome numbers in higher plants, with special reference to cytokinesis. Cytologia1936;7:544-95.

  15. Ahuja MR. Chromosome number of some medicinal plants. In J Genet Plant Breed 1955;15:142-3.

  16. Mall LP, Arzare KC. Autecological study of Achyranthes asperaLinn. Bull Bot Soc Uni Saugar1956;8:69-76.

  17. Sen S. Studies on the structure and behaviour of chromosomes of few species of the family Amaranthaceae. Proc 43rd Indian Sci Congr 1956;3:249.

  18. Thombre MV. Chromosome numbers in some common flowering plants. Sci Cult 1959;25:208.

  19. Roy RP, Thakur V. A comparative study of meiosis in two genera of the family Amaranthaceae. Proc 48th Indian Sci Congr 1961;3:303-4.

  20. Roy RP, Thakur V. On a polyploidy Achyranthes bidentataBl. (2n=48) in nature. Proc 49th Indian Sci Congr 1962;3:335-6.

  21. Pal M. Chromosome number in some Indian angiosperms. I. Proc Indian Acad Sci1964;60:347-50.

  22. Sharma AK, Banik M. Cytological investigations of different genera of amaranthaceae with a view to trace their inter-relationships. Bull Bot Soc Bengal 1965;19:40-50.

  23. Gill BS, Vasudeva S. Cytological studies in some members of amaranthaceae. Curr Sci 1970;39:445-6.

  24. Sharma AK. Annual report 1967-68. Res Bull Univ Calcutta (Cytogenetics lab) 1970;2:1-50.

  25. Kurosawa S. Cytological studies on some Eastern Himalayan plants and their related species. In: Hara H. editor. The flora of Eastern Himalaya. 2nd Report.Tokyo: University of Tokyo Press; 1971. p. 356-64.

  26. Mittra R. Cytology of amaranthaceae. Proc 58th Indian Sci Congr 1971;3:470-1.

  27. Miege J, Josserand N. Nombres chromosomiques d’ especes africannes et malgaches. Candollea 1972;27:283-92.

  28. Behera B, Patnaik SN. Cytotaxonomic studies in the family amaranthaceae. Cytologia1974;39:121-37.

  29. Van Loon JC. A cytological investigation of flowering plants from canary Island. Acta Bot Neerl 1974;23:113-24.

  30. Koul AK, Wakhlu AK, Karihaloo JL. Chromosome number of some flowering plants of Jammu (Western Himalayas) II. CIS Chromosome Inf Serv1976;20:32-3.

  31. Sidhu MK. Distributional and cytological studies of weed flora of cultivable fields of patiala district (Punjab).Ph. D. Thesis, Patiala; 1979. p. 1-230.

  32. Bir SS, Sidhu M. Cytological observations in weed flora of orchards of Patiala district, Punjab. Recent Res Adv Plant Sci1979;7:261-71.

  33. Bir SS, Sidhu M. Cytological observations on weed flora of orchards of Patiala district, Punjab. In: Bir SS. editor. Recent researches in plant sciences. Ludhiana (India): Kalyani Publishers; 1980. p. 261-71.

  34. Sidhu M, Bir SS. Karyological studies on cultivable lands in Punjab, India. Trop Plant Sci Res 1983;1:1-13.

  35. Dalgaard V. Chromosome numbers in flowering plants from Madeira. Willdenowia1986;16:221-40.

  36. Ge CJ, Li YK, Wan P, Hsu PS. Chromosome numbers of 31 medicinal plants from Shndong Province. In: Hong D. editor. Plant chromosome research 1987.Organising Committee Sino-Japanese Symposium on plant Chromosomes, Beijing; 1989. p. 267-72.

  37. Ge CJ, Li YK, Wan P, Li YX, Jiang FH. Observations on the chromosome numbers of medicinal plants from shandong province (V). J Shandong Coll Trad Chin Med 1988;12:55-7.

  38. Morton JK. Chromosome numbers and polyploidy in the flora of Cameroon mountain. Opera Bot1993;121:159-72.

  39. Malik NA, Ahmed AJ. Chromosome number in some medicinal plants. Pak J Sci Res1963;15:58-60.

  40. Sinha SSN, Prasad R, Keswani R. Cytological studies in some medicinal plants of Chhotanagpur. Proc 59th Indian Sci Congr 1972;3:349.

  41. Kumar A. Polyploidy in Achyranthes asperaand Chrysanthemum leucenthemum. Acta Bot Indica 1982;10:141-2.

  42. Mittra R. Cytology of amaranthaceae. Proc 58th Indian Sci Congr 1971;3:470-1.

  43. Lu CL, Zhu W, Wang M, Xu XJ, Lu CJ. Antioxidant and anti-inflammatory activities of phenolic-enriched extracts of Smilax glabra. J Evidence-Based Complementary Altern Med 2014:1-8.http://dx.doi.org/10.1155/2014/910438

  44. Lim JH, Park JW, Kim MS, Park SK, Johnson RS, Chun YS. Bafilomycin induces the p21-mediated growth inhibition of cancer cells under hypoxic conditions by expressing HIF-1α. Mol Pharmacol 2006;70:1856-65.

  45. BarrosMP,LemosM,MaistroEL,LeiteMF,SousaJP,BastosJK,et al. Evaluation of antiulcer activity of the main phenolic acids found in Brazilian green propolis. J Ethnopharmacol 2008;120:372-7.

  46. Cuong TD, Hung TM, Lee JS, Weon KY, Woo MH, Min BS. Anti-inflammatory activity of phenolic compounds from the whole plant of Scutellaria indica. Bioorg Med Chem Lett 2015;25:1129-34.

  47. Yu ZF, Kong LD, Chen Y. Antidepressant activity of aqueous extracts of Curcuma longa in mice. J Ethnopharmacol 2002;83:161-5.

  48. Lin SQ, Zhou ZL,  Zhang HL, Yin WQ. Phenolic glycosides from the rhizomes of Cyperus rotundus and their antidepressant activity.J Korean Soc Appl Biol Chem 2015;58:685-91.

  49. Saravanan P, Ramasamy V, Shivakumar T. Antimicrobial activity of leaf extracts of Achyranthes asperaLinn.Asian J Chem 2008;20:823-5.

  50. Joshi B, Sah GP, Basnet BB, Bhatt MR, Sharma D, Subedi K, et al.Phytochemical extraction and antimicrobial properties of different medicinal plants:Ocimum sanctum (Tulsi), Eugenia caryophyllata(Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem).J Microbiol Antimicrob 2011;3:1-7.

  51. Amrutia JN, Patel J, Semuel MR, Shabaraya AR. Antiinflammatory activity of fractionated extracts of Achyranthes aspera Linn. leaves. J Appl Pharm Sci 2011;1:188-90.

  52. Edwin S, Jarald EE, Deb L, Jain A, Kinger H, Dutt KR, et al. Wound healing and antioxidant activity of Achyranthes aspera. Pharm Biol 2008;46:824-8.

  53. Barua CC, Talukdar A, Begum SA, Buragohain B, Roy JD, Borah RS, et al. Antidepressant-like effects of the methanolic extract of Achyranthes aspera Linn. in animal models of depression. Pharmacologyonline 2009;2:587-94.

  54. Chakraborty A, Brantner A, Mukuinaka T, Nobukuni Y, Kuchido M, Konoshima T. Cancer chemo preventive activity of Achyranthes aspera leaves on Epstein-Barr virus activation and two stage mouse skin carcinogenesis. Cancer Lett 2002;177:1–5.

  55. Mehta FA, Patel BG, Pandya SS, Ahir KB, Patel SB.Antinociceptive and anti inflammatory activity of Achyranthes aspera L. extracts. Pharmacologyonline 2009;3:978-85.

  56. Kumar H, Singh D, Kushwaha SKS, Gupta AK. Comparison of leaf and root extract of Achyranthes aspera for its analgesic activity. Pharm Lett2009;1:193-8.

  57. Shibeshi W, Makonnen E, Zerihun L, Debella A. Effect of Achyranthes aspera L. on fetal abortion, uterine and pituitary weights, serum lipids and hormones. Afr Health Sci 2006;6:108-12.

  58. Tahiliani P, Kar A. Achyranthes aspera elevates thyroid hormone levels and decreases hepatic lipid peroxidation in male rats. J Ethnopharmacol 2000;71:527-32.

  59. Niranjan S, Alok DK, Soumya MK, Priyanka G, Susri MS. Diuretic activity of Achyranthes aspera leaves extract. Int Res JPharm 2012;3:216-8.

  60. RafiqkhanM, Saranya. Pharmacognostic profile and phytochemical investigation on the leaves of Achyranthes aspera.Int J Pharm Pharm Sci 2013;5:368-70.

  61. Hossain MJ, Khaleda L, Chowdhury AMMA, Arifuzzaman M, Al-ForkanM.Phytochemical screening and evaluation of cytotoxicity and thrombolytic properties of Achyranthes asperaleaf extract. JPharm Biol Sci 2013;6:30-8.

  62. Priya CL, Kumar G, Karthik L, Bhaskara Rao KV. Phytochemical composition and in vitro antioxidant activity of Achyranthes aspera Linn. (Amaranthaceae) leaf extract. Int J Agric Technol 2012;8:143-56.

  63. Zhongbing L, Guangjun B, Peter S, Belton C, Huiru D. Structure–activity relationship analysis of antioxidant ability and neuroprotective effect of gallic acid derivatives. Neurochem Int 2006;48:263-74.

  64. Chia YC, Rajbanshi R, Calhoun C, Chiu RH. Anti-neoplastic effects of gallic acid, a major component of Toona sinensisleaf extract, on oral squamous carcinoma cells. Molecules 2010;15:8377-89.

  65. Raina K, Rajamanickam S, Deep G, Singh M, Agarwal R, Agarwal C. Chemopreventive effects of oral gallic acid feeding on tumor growth and progression in TRAMP mice. Mol Cancer Ther 2008;7:1258-67.

  66. Sohi KK, Mittal N, Hundal MK, Khanduja KL. Gallic acid, an antioxidant, exhibits antiapoptotic potential in normal human lymphocytes: a Bcl-2 independent mechanism. J Nutr Sci Vitaminol 2003;49:221-7.

  67. Patel SS, Goyal RK. Cardioprotective effects of gallic acid in diabetes-induced myocardial dysfunction in rats. Pharmacogn Res 2011;3:239-45.

How to cite this article

  • Manjit Inder Singh Saggoo, Lovleen. Screening of total phenol and flavonoid content in different cytotypes of two species of Achyranthes linn. from western himalaya, India.Int J Pharm Pharm Sci 2017;9(10):205-210.



About this article

Title

SCREENING OF TOTAL PHENOL AND FLAVONOID CONTENT IN DIFFERENT CYTOTYPES OF TWO SPECIES OF ACHYRANTHES LINN. FROM WESTERN HIMALAYA, INDIA

Keywords

Achyranthes Linn, Cytotypes, Total phenols, Total flavonoids, Western Himalaya

DOI

10.22159/ijpps.2017v9i10.19780

Date

02-10-2017

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Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol. 9, Issue 10, 2017 Page: 205-210

Online ISSN

0975-1491

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Authors & Affiliations

Manjit Inder Singh Saggoo
Department of Botany, Punjabi University, Patiala-147002, Punjab, India
India

Lovleen .
Department of Botany, DAV College, Jalandhar- 144008, Punjab, India
India


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