SCREENING OF TOTAL PHENOL AND FLAVONOID CONTENT IN DIFFERENT CYTOTYPES OF TWO SPECIES OF ACHYRANTHES LINN. FROM WESTERN HIMALAYA, INDIA
Keywords:Achyranthes Linn, Cytotypes, Total phenols, Total flavonoids, Western Himalaya
Objective: Genus Achyranthes Linn. belonging to family Amaranthaceae consists of six species. The present study was undertaken to screen the phenolic components in the different cytotypes of two species of Genus Achyranthes Linn. growing in western Himalaya, India.
Methods: Methanol extract of leaves was used to determine the total phenol and flavonoid contents in different cytotypes of A. aspera Linn. and A. bidentata Blume by spectrophotometric method. Total phenol content was expressed as mg gallic acid g-1phenol and total flavonoid content as mg quercetin g-1flavonoid using the standard curves. Further, gallic acid content in methanol extracts of leaves was determined by RP-HPLC method using C-18 column, employing 0.01% (v/v) orthophosphoric acid: acetonitrile (98:2 v/v) as mobile phase at a flow rate of 1 ml/min with ultraviolet (UV) detection at 272 nm.
Results: Hexaploid plants of A. aspera Linn. possess the higher amount of phenol (9.16Â±0.84 mg/g) and flavonoid (78.36Â±1.63 mg/g) constituents in the methanol extract of leaves as compared to its dodecaploid counterparts (7.86Â±0.08 mg/g and 70.20Â±1.81 mg/g respectively). Similarly, phenol and flavonoid content is found to be more in the methanol extract of leaves of hexaploid plants of A. bidentata Blume (11.93Â±0.59 mg/g and 115.92Â±1.32 mg/g respectively) as compared to its dodecaploid counterparts (9.46Â±0.75 mg/g and 107.76Â±0.94 mg/g respectively). Further, RP-HPLC analysis of gallic acid revealed that higher amount of gallic acid is present in dodecaploid plants of A. aspera Linn. (1.04Â±0.02 mg/g) and A. bidentata Blume (1.34Â±0.03 mg/g) as compared to hexaploid counterparts (1.01Â±0.01 mg/g and 1.22Â±0.05 mg/g respectively).
Conclusion: The present investigation revealed that A. aspera Linn. and A. bidentata Blume plants show immense intraspecific variability in their phenolic components. Hence there is need to evaluate germplasm to select superior genotype for medicinal and conservation purpose.
Harwood RK, Palmer J. Amaranthaceae. In: Short PS, Cowie ID. editors. Flora of the Darwin region. Vol. 1. Australia: Northern Territory Herbarium, Department of Natural Resources, Environment, the Arts, and Sport; 2011. p. 1-23.
Nadkarni KM. Indian materia medica. Vol. I. Bombay Popular Prakashan; 2009. p. 21.
Singh VK, Ali ZA, Zaidi STH, Siddiqui MK. Ethnomedicinal uses of plants from gonda district forests of Uttar Pradesh, India. Fitoterapia 1996;67:129-39.
Gupta RK. Medicinal and aromatic plants. CBS publishers and distributors; 2010. p. 190.
Bhattarai NK. Folk herbal remedies of sindhupalchok district, central Nepal. Fitoterapia 1992;63:145-55.
Guha Bakashi DN, Sensarma P, Pal DC. A lexicon of medicinal plants in India. Vol. I. Calcutta (India): Naya Prokash (New Sarada Press); 1999.
Dhiman AK. Banaras ayurveda series 2-medicinal plants of Uttranchal state. Varanasi (India): Chowkhamba Press; 2004.
Pullaiah T. Encyclopedia of world medicinal plants. Vol. I. New Delhi (India): Regency Publications; 2006.
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem 2003;51:6509-15.
Rakesh SU, Patil PR, Salunkhe VR, Dhabale PN, Burade KB, Bhise SB. Quantitative estimation of gallic acid from hydroalcoholic extract of dried flowers of Nymphaea stellata willd by RP-HPLC. Asian J Chem 2010;22:5919-24.
Darlington CD, Wyile AP. Chromosome atlas of flowering plants. London: George Allen and Unwin Ltd; 1955.
Sugiura T. A list of chromosome numbers in angiospermous plants. Bot Mag 1931;42:504-6.
Sugiura T. Studies on the chromosome numbers in higher plants, with special reference to cytokinesis. Cytologia 1936;7:544-95.
Ahuja MR. Chromosome number of some medicinal plants. In J Genet Plant Breed 1955;15:142-3.
Mall LP, Arzare KC. Autecological study of Achyranthes aspera Linn. Bull Bot Soc Uni Saugar 1956;8:69-76.
Sen S. Studies on the structure and behaviour of chromosomes of few species of the family Amaranthaceae. Proc 43rd Indian Sci Congr 1956;3:249.
Thombre MV. Chromosome numbers in some common flowering plants. Sci Cult 1959;25:208.
Roy RP, Thakur V. A comparative study of meiosis in two genera of the family Amaranthaceae. Proc 48th Indian Sci Congr 1961;3:303-4.
Roy RP, Thakur V. On a polyploidy Achyranthes bidentata Bl. (2n=48) in nature. Proc 49th Indian Sci Congr 1962;3:335-6.
Pal M. Chromosome number in some Indian angiosperms. I. Proc Indian Acad Sci 1964;60:347-50.
Sharma AK, Banik M. Cytological investigations of different genera of amaranthaceae with a view to trace their inter-relationships. Bull Bot Soc Bengal 1965;19:40-50.
Gill BS, Vasudeva S. Cytological studies in some members of amaranthaceae. Curr Sci 1970;39:445-6.
Sharma AK. Annual report 1967-68. Res Bull Univ Calcutta (Cytogenetics lab) 1970;2:1-50.
Kurosawa S. Cytological studies on some Eastern Himalayan plants and their related species. In: Hara H. editor. The flora of Eastern Himalaya. 2nd Report. Tokyo: University of Tokyo Press; 1971. p. 356-64.
Mittra R. Cytology of amaranthaceae. Proc 58th Indian Sci Congr 1971;3:470-1.
Miege J, Josserand N. Nombres chromosomiques dâ€™ especes africannes et malgaches. Candollea 1972;27:283-92.
Behera B, Patnaik SN. Cytotaxonomic studies in the family amaranthaceae. Cytologia 1974;39:121-37.
Van Loon JC. A cytological investigation of flowering plants from canary Island. Acta Bot Neerl 1974;23:113-24.
Koul AK, Wakhlu AK, Karihaloo JL. Chromosome number of some flowering plants of Jammu (Western Himalayas) II. CIS Chromosome Inf Serv 1976;20:32-3.
Sidhu MK. Distributional and cytological studies of weed flora of cultivable fields of patiala district (Punjab). Ph. D. Thesis, Patiala; 1979. p. 1-230.
Bir SS, Sidhu M. Cytological observations in weed flora of orchards of Patiala district, Punjab. Recent Res Adv Plant Sci 1979;7:261-71.
Bir SS, Sidhu M. Cytological observations on weed flora of orchards of Patiala district, Punjab. In: Bir SS. editor. Recent researches in plant sciences. Ludhiana (India): Kalyani Publishers; 1980. p. 261-71.
Sidhu M, Bir SS. Karyological studies on cultivable lands in Punjab, India. Trop Plant Sci Res 1983;1:1-13.
Dalgaard V. Chromosome numbers in flowering plants from Madeira. Willdenowia 1986;16:221-40.
Ge CJ, Li YK, Wan P, Hsu PS. Chromosome numbers of 31 medicinal plants from Shndong Province. In: Hong D. editor. Plant chromosome research 1987. Organising Committee Sino-Japanese Symposium on plant Chromosomes, Beijing; 1989. p. 267-72.
Ge CJ, Li YK, Wan P, Li YX, Jiang FH. Observations on the chromosome numbers of medicinal plants from shandong province (V). J Shandong Coll Trad Chin Med 1988;12:55-7.
Morton JK. Chromosome numbers and polyploidy in the flora of Cameroon mountain. Opera Bot 1993;121:159-72.
Malik NA, Ahmed AJ. Chromosome number in some medicinal plants. Pak J Sci Res 1963;15:58-60.
Sinha SSN, Prasad R, Keswani R. Cytological studies in some medicinal plants of Chhotanagpur. Proc 59th Indian Sci Congr 1972;3:349.
Kumar A. Polyploidy in Achyranthes aspera and Chrysanthemum leucenthemum. Acta Bot Indica 1982;10:141-2.
Mittra R. Cytology of amaranthaceae. Proc 58th Indian Sci Congr 1971;3:470-1.
Lu CL, Zhu W, Wang M, Xu XJ, Lu CJ. Antioxidant and anti-inflammatory activities of phenolic-enriched extracts of Smilax glabra. J Evidence-Based Complementary Altern Med 2014:1-8. http://dx.doi.org/10.1155/2014/910438
Lim JH, Park JW, Kim MS, Park SK, Johnson RS, Chun YS. Bafilomycin induces the p21-mediated growth inhibition of cancer cells under hypoxic conditions by expressing HIF-1Î±. Mol Pharmacol 2006;70:1856-65.
Barros MP, Lemos M, Maistro EL, Leite MF, Sousa JP, Bastos JK, et al. Evaluation of antiulcer activity of the main phenolic acids found in Brazilian green propolis. J Ethnopharmacol 2008;120:372-7.
Cuong TD, Hung TM, Lee JS, Weon KY, Woo MH, Min BS. Anti-inflammatory activity of phenolic compounds from the whole plant of Scutellaria indica. Bioorg Med Chem Lett 2015;25:1129-34.
Yu ZF, Kong LD, Chen Y. Antidepressant activity of aqueous extracts of Curcuma longa in mice. J Ethnopharmacol 2002;83:161-5.
Lin SQ, Zhou ZL, Zhang HL, Yin WQ. Phenolic glycosides from the rhizomes of Cyperus rotundus and their antidepressant activity. J Korean Soc Appl Biol Chem 2015;58:685-91.
Saravanan P, Ramasamy V, Shivakumar T. Antimicrobial activity of leaf extracts of Achyranthes aspera Linn. Asian J Chem 2008;20:823-5.
Joshi B, Sah GP, Basnet BB, Bhatt MR, Sharma D, Subedi K, et al. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). J Microbiol Antimicrob 2011;3:1-7.
Amrutia JN, Patel J, Semuel MR, Shabaraya AR. Antiinflammatory activity of fractionated extracts of Achyranthes aspera Linn. leaves. J Appl Pharm Sci 2011;1:188-90.
Edwin S, Jarald EE, Deb L, Jain A, Kinger H, Dutt KR, et al. Wound healing and antioxidant activity of Achyranthes aspera. Pharm Biol 2008;46:824-8.
Barua CC, Talukdar A, Begum SA, Buragohain B, Roy JD, Borah RS, et al. Antidepressant-like effects of the methanolic extract of Achyranthes aspera Linn. in animal models of depression. Pharmacologyonline 2009;2:587-94.
Chakraborty A, Brantner A, Mukuinaka T, Nobukuni Y, Kuchido M, Konoshima T. Cancer chemo preventive activity of Achyranthes aspera leaves on Epstein-Barr virus activation and two stage mouse skin carcinogenesis. Cancer Lett 2002;177:1â€“5.
Mehta FA, Patel BG, Pandya SS, Ahir KB, Patel SB. Antinociceptive and anti inflammatory activity of Achyranthes aspera L. extracts. Pharmacologyonline 2009;3:978-85.
Kumar H, Singh D, Kushwaha SKS, Gupta AK. Comparison of leaf and root extract of Achyranthes aspera for its analgesic activity. Pharm Lett 2009;1:193-8.
Shibeshi W, Makonnen E, Zerihun L, Debella A. Effect of Achyranthes aspera L. on fetal abortion, uterine and pituitary weights, serum lipids and hormones. Afr Health Sci 2006;6:108-12.
Tahiliani P, Kar A. Achyranthes aspera elevates thyroid hormone levels and decreases hepatic lipid peroxidation in male rats. J Ethnopharmacol 2000;71:527-32.
Niranjan S, Alok DK, Soumya MK, Priyanka G, Susri MS. Diuretic activity of Achyranthes aspera leaves extract. Int Res J Pharm 2012;3:216-8.
Rafiqkhan M, Saranya. Pharmacognostic profile and phytochemical investigation on the leaves of Achyranthes aspera. Int J Pharm Pharm Sci 2013;5:368-70.
Hossain MJ, Khaleda L, Chowdhury AMMA, Arifuzzaman M, Al-Forkan M. Phytochemical screening and evaluation of cytotoxicity and thrombolytic properties of Achyranthes aspera leaf extract. J Pharm Biol Sci 2013;6:30-8.
Priya CL, Kumar G, Karthik L, Bhaskara Rao KV. Phytochemical composition and in vitro antioxidant activity of Achyranthes aspera Linn. (Amaranthaceae) leaf extract. Int J Agric Technol 2012;8:143-56.
Zhongbing L, Guangjun B, Peter S, Belton C, Huiru D. Structureâ€“activity relationship analysis of antioxidant ability and neuroprotective effect of gallic acid derivatives. Neurochem Int 2006;48:263-74.
Chia YC, Rajbanshi R, Calhoun C, Chiu RH. Anti-neoplastic effects of gallic acid, a major component of Toona sinensis leaf extract, on oral squamous carcinoma cells. Molecules 2010;15:8377-89.
Raina K, Rajamanickam S, Deep G, Singh M, Agarwal R, Agarwal C. Chemopreventive effects of oral gallic acid feeding on tumor growth and progression in TRAMP mice. Mol Cancer Ther 2008;7:1258-67.
Sohi KK, Mittal N, Hundal MK, Khanduja KL. Gallic acid, an antioxidant, exhibits antiapoptotic potential in normal human lymphocytes: a Bcl-2 independent mechanism. J Nutr Sci Vitaminol 2003;49:221-7.
Patel SS, Goyal RK. Cardioprotective effects of gallic acid in diabetes-induced myocardial dysfunction in rats. Pharmacogn Res 2011;3:239-45.