INFLUENCE OF PHYSICO-CHEMICAL PARAMETERS ON THE FABRICATION OF SILVER NANOPARTICLES USING MARANTA ARUNDINACEA L AND ITS ANTI-MICROBIAL EFFICACY
DOI:
https://doi.org/10.22159/ijpps.2025v17i3.28365Keywords:
Silver nanoparticles, Maranta arundinacea L, Physico-chemical parameters, Antimicrobial activityAbstract
Objective: To investigate phytosynthesis of silver nanoparticles, factors governing the synthesis of nanoparticles and antibacterial activity of synthesized silver nanoparticles.
Methods: The leaf extract of Maranta arundinacea L. was used for synthesis of silver nanoparticles which was confirmed by changing the colour of reaction mixture from colourless to brown. Synthesized silver nanoparticles were characterized by UV-vis spectroscopy(UV-vis), Fourier Transform Infra Red Spectrophotometer (FTIR), X-ray Diffraction (XRD), Energy dispersive X-ray diffraction (EDX), Atomic Force Microscopy (AFM), High Resolution Transmission Electron Microscopy (HR-TEM) and testing the antibacterial activity against E. coli, S. typhi, S. aureus and B. polymyxa following well diffusion method.
Results: Silver nanoparticles shows characteristic UV-Vis absorption peak at 406 nm. The influence of physico-chemical parameters was studied and optimized to obtain nanoparticles of diverse sizes. The size of silver nanoparticles ranges from 30 to 90 nm and were found to be spherical in shape with crystalline nature. The synthesized silver nanoparticles showed good antimicrobial activity against multi-drug resistant S. aureus, B. polymyxa, E. coli and Salmonella typhi. Silver nanoparticles show that higher antibacterial activity was observed in Gram negative bacteria than the Gram-positive bacteria. The highest zone of inhibition 7.033±0.033 mm was observed in S. typhi at 250 µL and lowest zone of inhibition 4.100±0.066 mm was seen in B. polymyxa at 250 µL.
Conclusion: Silver nanoparticles synthesized using leaf extract of Maranta arundinacea L. may exhibit reasonable testing antimicrobial activity against some pathogenic microbes.
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