Preliminary phytochemical analysis, Antioxidant and Antimicrobial evaluation of Carthamus lanatus


Sania Feroz

Abstract


Objectives: This study is aimed at examining alkaloids, tannins, saponins, flavonoids and steroids contents in methanolic extract of Carthamus lanatus and their antimicrobial (antifungal and antibacterial) and antioxidant activities.

Methods: The conventional phytochemical screening tests were used for the identification of secondary metabolites. Antioxidant, antifungal and antibacterial activities were carried out by using DPPH free radical scavenging method, agar tube dilution and agar well diffusion methods, respectively.

Results: Phytochemical screening showed the presence of alkaloids, tannins, saponins, flavonoids, caumarins, steroids and betacyanins. The antimicrobial activities of C. lanatus in methanol solvent were tested against gram +ve (Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis) and gram –ve (Escherichia coli) organisms as well as the fungus (Aspergillus niger, Aspergillus flavus and Alternaria solani). For antimicrobial test, well diffusion technique was used and the zone of inhibition of microorganisms was measured in mm. Antioxidant assay measures by spectrophotometer the ability of antioxidants to reduce 2, 2- diphenylpicrylhydrazyl (DPPH), another radical not commonly found in biological systems.

Conclusion: C. lanatus is a very good antioxidant and antimicrobial plant. The presence of various secondary metabolites makes it a very significant plant for various activities.


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About this article

Title

Preliminary phytochemical analysis, Antioxidant and Antimicrobial evaluation of Carthamus lanatus

Date

21-10-2016

Additional Links

Manuscript Submission

Journal

Innovare Journal of Sciences
Vol 4 Issue 5 October-November 2016 Page:

Online ISSN

2321-5496

Statistics

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Authors & Affiliations

Sania Feroz
Allama Iqbal open university Islamabad, Pakistan
Pakistan


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