RP-HPLC METHOD FOR ESTIMATION OF AN ANTIPSYCHOTIC DRUG - PIMOZIDE
AbstractObjective â€“ To develop and validate RP-HPLC method for estimation of Pimozide in bulk and its tablet dosage form. Method- Chromatographic separation was carried out on Grace Smart RP-18 column (250 mm X 4.6 mm inner diameter; 5Âµm particle size) using a mobile phase consisted of Acetonitrile: 50 mM Disodium hydrogen phosphate buffer (pH 6.2, adjusted by 1 % ortho phosphoric acid) in the ratio of 60: 40 %v/v. The flow rate was maintained at 1 ml/min and UV detection was measured at 280 nm. Propyphenazone was used as an internal standard. Results-The calibration curve of Pimozide was linear in range of 5- 100 Âµg/ml. The mean % assay of marketed formulation was found to be 101.02 % and % recovery was observed in the range of 99.23- 101.91 %. Relative standard deviation for precision study was found less than 2 %. The LOD and LOQ values were found to be 0.553 Âµg/ml and 1.678 Âµg/ml respectively. Conclusion- The developed method is simple rapid and easy to apply, making it suitable for routine analysis of Pimozide in bulk and tablet dosage form. KEYWORDS- Pimozide, RP-HPLC, Validation
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How to Cite
Kabra, P., L. Nargund, and M. Srinivasa Murthy. “RP-HPLC METHOD FOR ESTIMATION OF AN ANTIPSYCHOTIC DRUG - PIMOZIDE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 7, May 2014, pp. 49-51, https://innovareacademics.in/journals/index.php/ajpcr/article/view/1023.
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