SCREENING OF NINE HERBAL PLANTS FOR ALPHA-AMYLASE INHIBITION

Authors

  • RITUPARNA CHAKRABARTI VIT University, Vellore
  • BHAVTARAN SINGH VIT University, Vellore
  • PRAKRITH VN VIT University, Vellore
  • LALTHANZAMA VANCHHAWNG VIT University, Vellore
  • KAVITHA THIRUMURUGAN School of Bio Sciences & TechnologyCentre for Biomedical ResearchVIT UniversityVellore632014

Abstract

Objective: To evaluate the α-amylase inhibitory potential of nine herbal plants in regulating postprandial hyperglycemia.
Materials and Methods: In vitro α-amylase inhibition assay using starch-iodine was performed. α-amylase inhibition delays breakdown of starch and
prevents glucose release to reduce postprandial hyperglycemia.
Results: The plants screened were Artocarpus altilis, Aconitum heterophyllum, Acorus calamus, Berberis aristata, Cassia auriculata, Cyprus rotundus,
Mesua ferrea, Plumbago zeylanicum and Terminalia arjuna. Positive control Acarbose showed IC50 at 14.24 μg/ml. Methanolic extract of C. auriculata
(flower), T. arjuna (bark) and P. zeylanicum (rhizome) exhibited the best inhibitory activity with IC50 value of 37.28 μg/ml, 48.75 μg/ml and
68.66 μg/ml, respectively.
Conclusion: From the present study, we conclude that C. auriculata flower had displayed maximum inhibition against α-amylase.

Keywords: Herbal plants, Hyperglycemia, α-amylase inhibition.

Author Biography

KAVITHA THIRUMURUGAN, School of Bio Sciences & TechnologyCentre for Biomedical ResearchVIT UniversityVellore632014

School of Bio Sciences & Technology

Rank 1

References

Al Kazaz M, Desseaux V, Marchis-Mouren G, Prodanov E,

Santimone M. The mechanism of porcine pancreatic alpha-amylase.

Inhibition of maltopentaose hydrolysis by acarbose, maltose and

maltotriose. Eur J Biochem 1998;252(1):100-7.

Buisson G, Duée E, Haser R, Payan F. Three dimensional structure of

porcine pancreatic alpha-amylase at 2.9 A resolution. Role of calcium

in structure and activity. EMBO J 1987;6(13):3909-16.

Zhuo H, Payan F, Qian M. Crystal structure of the pig pancreatic alphaamylase

complexed with rho-nitrophenyl-alpha-D-maltoside-flexibility

in the active site. Protein J 2004;23(6):379-87.

Qian M, Ajandouz el H, Payan F, Nahoum V. Molecular basis of the

effects of chloride ion on the acid-base catalyst in the mechanism of

pancreatic alpha-amylase. Biochemistry 2005;44(9):3194-201.

Robyt JF, French D. The action pattern of porcine pancreatic alphaamylase

in relationship to the substrate binding site of the enzyme.

J Biol Chem 1970;245(15):3917-27.

Seigner C, Prodanov E, Marchis-Mouren G. On porcine pancreatic

alpha-amylase action: kinetic evidence for the binding of two

maltooligosaccharide molecules (maltose, maltotriose and

o-nitrophenylmaltoside) by inhibition studies. Correlation with the

five-subsite energy profile. Eur J Biochem 1985;148(1):161-8.

Krentz AJ, Bailey CJ. Oral antidiabetic agents: Current role in type 2

diabetes mellitus. Drugs 2005;65(3):385-411.

Sudha P, Zinjarde SS, Bhargava SY, Kumar AR. Potent α-amylase

inhibitory activity of Indian Ayurvedic medicinal plants. BMC

Complement Altern Med 2011;11:5.

Ashok Kumar BS, Lakshman K, Nandeesh R, Arun Kumar PA, Manoj B,

Kumar V, et al. In vitro alpha-amylase inhibition and in vivo antioxidant

potential of Amaranthus spinosus in alloxan-induced oxidative stress in

diabetic rats. Saudi J Biol Sci 2011;18(1):1-5.

Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of

diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010;87(1):4-14.

Ceriello A. Postprandial hyperglycemia and diabetes complications: Is

it time to treat? Diabetes 2005;54(1):1-7.

Nyenwe EA, Jerkins TW, Umpierrez GE, Kitabchi AE. Management of

type 2 diabetes: Evolving strategies for the treatment of patients with

type 2 diabetes. Metabolism 2011;60(1):1-23.

Bachhawat A, Shihabudeen MS, Thirumurugan K. Screening of fifteen

Indian ayurvedic plants for alpha-glucosidase inhibitory activity and

enzyme kinetics. Int J Pharm Pharm Sci 2011;3 Suppl 4:267-74.

Mohamed Sham Shihabudeen H, Hansi Priscilla D, Thirumurugan K.

Cinnamon extract inhibits a-glucosidase activity and dampens

postprandial glucose excursion in diabetic rats. Nutr Metab (Lond)

;8(1):46.

Bonora E, Muggeo M. Postprandial blood glucose as a risk factor

for cardiovascular disease in type II diabetes: The epidemiological

evidence. Diabetologia 2001;44(12):2107-14.

Hollander P. Safety profile of acarbose, an alpha-glucosidase inhibitor.

Drugs 1992;44 Suppl 3:47-53.

Kare CP. Indian Medicinal Plants: An Illustrated Dictionary. New York,

Berlin, Heidelberg: Springer Verlag; 2007.

Ragone D. Artocarpus altilis (breadfruit). Species profiles for pacific

island agroforestry; 2006. p. 1-17.

Lather A. Pharmacological potential of ayurvedic formulation:Kutajghan vati-A review. J Adv Sci Res 2010;1(2):41-5.

Venkatasubramanian P, Kumar SK, Nair VS. Cyperus rotundus, a

substitute for Aconitum heterophyllum: Studies on the ayurvedic

concept of Abhava Pratinidhi Dravya (drug substitution). J Ayurveda

Integr Med 2010;1(1):33-9.

Atal CK, Sharma ML, Kaul A, Khajuria A. Immunomodulating

agents of plant origin. I: Preliminary screening. J Ethnopharmacol

;18(2):133-41.

Mazumder PM, Das S, Das MK. Cytotoxic activity of methanolic

extracts of Berberis aristata DC and Hemidesmus indicus R.Br. in

MCF7 cell line. J Curr Pharm Res 2010;1:12-5.

Gilani AU, Janbaz KH. Preventive and curative effects of Berberis

aristata Fruit extract on paracetamol and CCl4-induced hepatotoxicity.

Phytother Res 1995;9(7):489-94.

Singh J, Kakkar P. Antihyperglycemic and antioxidant effect of Berberis

aristata root extract and its role in regulating carbohydrate metabolism

in diabetic rats. J Ethnopharmacol 2009;123(1):22-6.

Chakrabarti R, Singh B, Narendra P, Varghese N, Vanchhawng L,

Shihabudeen MS, et al. Dipeptidyl peptidase-IV inhibitory activity of

Berberis aristata. J Nat Prod 2011;4:158-63.

Siva R, Krishnamurthy KV. Isozyme diversity in Cassia auriculata L.

Afr J Biotechnol 2005;4(8):772-5.

Dhanasekaran JJ, Ganapathy M. Hepatoprotective effect of Cassia

auriculata L. leaf extract on carbon tetrachloride intoxicated liver

damage in wistar albino rats. Asian J Biochem 2001;6(1):104-12.

Latha M, Pari L. Preventive effects of Cassia auriculata L. flowers on

brain lipid peroxidation in rats treated with streptozotocin. Mol Cell

Biochem 2003;243(1-2):23-8.

Kusano R, Ogawa S, Matsuo Y, Tanaka T, Yazaki Y, Kouno I. a-Amylase

and lipase inhibitory activity and structural characterization of acacia

bark proanthocyanidins. J Nat Prod 2011;74(2):119-28.

Sabu MC, Subburaju T. Effect of Cassia auriculata Linn. on serum

glucose level, glucose utilization by isolated rat hemidiaphragm.

J Ethnopharmacol 2002;80(2-3):203-6.

Raut NA, Gaikwad NJ. Antidiabetic activity of hydro-ethanolic extract

of Cyperus rotundus in alloxan induced diabetes in rats. Fitoterapia

;77(7-8):585-8.

Jalalpure SS, Mandavkar YD, Khalure PR, Shinde GS, Shelar PA,

Shah AS. Antiarthritic activity of various extracts of Mesua ferrea Linn.

seed. J Ethnopharmacol 2011;138(3):700-4.

Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd Revised ed.,

Vol. 8. New Delhi: Indian Council Medical Research; 2012.

Shome U, Mehrotra S, Sharma HP. Pharmacognostic studies on the

flower of Mesua ferrea L. Proc Plant Sci 1982;91(3):211-26.

Meherji PK, Shetye TA, Munshi SR, Vaidya RA, Antarkar DS,

Koppikar S, et al. Screening of Mesua ferrea (Nagkesar) for

estrogenic & progestational activity in human & experimental models.

Indian J Exp Biol 1978;16(8):932-3.

Chetty KM, Sivaji K, Sudarsanam G, Hindu Sekar P. Pharmaceutical

studies and therapeutic uses of plumbago zeylanica L. Roots

(Chitraka,Chitramulamu). Ethnobotanical Lealf 2006;10:294-304.

Teklehaymanot T, Giday M. Ethnobotanical study of medicinal plants

used by people in Zegie Peninsula, Northwestern Ethiopia. J Ethnobiol

Ethnomed 2007;3:12.

Checker R, Sharma D, Sandur SK, Khanam S, Poduval TB. Antiinflammatory

effects of plumbagin are mediated by inhibition

of NF-kappaB activation in lymphocytes. Int Immunopharmacol

;9(7‑8):949-58.

Pettit GR, Hoard MS, Doubek DL, Schmidt JM, Pettit RK, Tackett LP,

et al. Antineoplastic agents 338. The cancer cell growth inhibitory.

Constituents of Terminalia arjuna (Combretaceae). J Ethnopharmacol

;53(2):57-63.

Maulik SK, Katiyar CK. Terminalia arjuna in cardiovascular diseases:

Making the transition from traditional to modern medicine in India.

Curr Pharm Biotechnol 2010;11(8):855-60.

Motley TJ. The ethnobotany of sweet flag, Acorus calamus (araceae).

Econ Bot 1994;48(4):397-412.

Agarwal SL, Arora RB, Dandiya PC, Singh KP. A note on the preliminary

studies of certain pharmacological actions of Acorus calamus L. J Am

Pharm Assoc Am Pharm Assoc (Baltim)1956;45(9):655-6.

Sies H, Stahl W, Sevanian A. Nutritional, dietary and postprandial

oxidative stress. J Nutr 2005;135(5):969-72.

Monnier L, Colette C. Contribution of fasting and post prandial glucose

to haemoglobin A1c. Endocr Pract 2006;12(1):42-6.

Published

2014-09-01

How to Cite

CHAKRABARTI, R., B. SINGH, P. VN, L. VANCHHAWNG, and K. THIRUMURUGAN. “SCREENING OF NINE HERBAL PLANTS FOR ALPHA-AMYLASE INHIBITION”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 4, Sept. 2014, pp. 84-89, https://innovareacademics.in/journals/index.php/ajpcr/article/view/1076.

Issue

Section

Original Article(s)