DETERMINATION OF MEDIAN LETHAL DOSE OF TRIAZOPHOS (TZ) WITH DMSO IN WISTAR RAT

  • Mohineesh Chandra All India Institute of Medical Sciences (AIIMS), New Delhi.
  • Jaya Raj All India Institute of Medical Sciences (AIIMS), New Delhi
  • Tirath Das Dogra SGT University, Budhera, Gurgao, India
  • Avinash Chandra Rajvanshi NICFS, Rohini, New Delhi-India
  • Anupuma Raina All India Institute of Medical Sciences (AIIMS), New Delhi

Abstract

The best poisons usually have three things in common: Small effective dose, also called Median lethal dose 50 (LD50), ease of administration
and rapid and definitive action. Median LD50 is usually an initial screening step in the assessment and evaluation of the toxic characteristic of a
substance. It is the amount (dose)of a chemical, calculated as per the concentration of chemicals, which produce death in 50% of the population of
test animals to which it is administered by any of the methods such as oral, dermal inhalation or intravenous. Determination of this test examines
the relationship between dose and the most extreme response-death. The more potent or toxic the chemical, lower the LD50 and the smaller the
dose needed to cause death. LD50 figures are frequently used as a general indicator of a substance’s acute toxicity. The present study was designed
to evaluate the LD50 of triazophos (TZ), an organophosphorus pesticide in Wistar rats. A single oral dose of TZ was dissolved in Dimethyl sulfoxide
(DMSO) and administered orally at a concentration of 35, 55, 90 and 100 mg/kg respectively to experimental animals. LD50 of the TZ in DMSO
was found to be 72.44 mg/kg. Single dose of TZ at 35 mg/kg did not reveal any toxic signs or behavioral alterations, hence it is considered as No
Observed Adverse Effect Level (NOAEL). LD50 is a standard measurement of acute toxicity. Knowledge of LD50 is very important for toxicologists
to correlate or to identify a substance or any poison and to measure the short-term poisoning potential (acute toxicity) for drugs, food poisonings
and accidental domestic poisonings cases etc. The knowledge gained from dose-response studies in animals is used to set standards for human
exposure.

Keywords: Preservative, Organophophorus, Triazophos, Butylatedhydroxyanisole, Lethal dose 50, No observed adverse effect level.

Author Biographies

Mohineesh Chandra, All India Institute of Medical Sciences (AIIMS), New Delhi.
FORENSIC MEDICINE AND TOXICOLOGY
Jaya Raj, All India Institute of Medical Sciences (AIIMS), New Delhi
Department of Forensic Medicine & Toxicology
Anupuma Raina, All India Institute of Medical Sciences (AIIMS), New Delhi
FORENSIC MEDICINE AND TOXICOLOGY

References

1. Raj J, Chandra M, Dogra TD, Pahuja M, Raina A. Determination of
median lethal dose of combination of endosulfan and cypermethrin in
wistar rat. Toxicol Int 2013;20(1):1-5.
2. Anwar WA. Biomarkers of human exposure to pesticides. Environ
Health Perspect 1997;105 Suppl 4:801-6.
3. Available from: http://www.bestchoicemall.com/
howtomakecompostguide/pesticide%20cycle.gif. [Last accessed on
2013 Sep 18].
4. Environmental Information System-National Institute of Occupational
Health 2007;2:1. (Available from http://sgh.org.sa/Portals/Articles/
Occupational and Environmental Health-Recognizing and Preventing
Disease and Injury.pdf. [Last accessed on 2013 Sep 18].
5. Miller LC, Tainter ML. Estimation of LD50 and its error by means of
log - Probit graph paper. Proc Soc Exp Biol Med 1944;57:261-4.
6. Bliss CI. The method of probits – A correction. Science
1934;79(2053):409-10.
7. Litchfield JT Jr, Wilcoxon F. A simplified method of evaluating doseeffect
experiments. J Pharmacol Exp Ther 1949;96(2):99-113.
8. Finney DJ. Probit Analysis. 3rd ed. Cambridge, UK: Cambridge
University Press; 1971.
9. Weil CS. Tables for convenient calculation of median effective
dose (LD50 or ED50) and instructions in their use. Biometrics
1951;8(3):249‑63.
10. Thompson WR. Use of moving averages and interpolation to estimate
median-effective dose; fundamental formulas, estimation of error, and
relation to other methods. Bacteriol Rev 1947;11(2):115-45.
11. Mohineesh C, Raina A, Raj J, Dogra TD. Development of new solvent
systems for the analysis of triazophos (O, O-diethyl O-1-phenyl-1H-1,
2, 4-triazol-3-yl phosphorothioate) extracted from blood. Proc Indian
Natl Sci Acad 2012;78(1):34.
12. Jain S, Mythily S, Ahmed RS, Arora VK, Banerjee BD. Induction of
oxidative stress and histopathological changes by sub-chronic doses of
triazophos. Indian J Biochem Biophys 2010;47(6):388-92.
13. Mingjing Q, Zhaojun H, Xinjun X, Lina Y. Triazophos resistance
mechanisms in the rice stem borer (Chilo suppressalis Walker). Pestic
Biochem Physiol 2003;77(3):99-105.
14. Kamanyire R, Karalliedde L. Organophosphate toxicity and
occupational exposure. Occup Med (Lond) 2004;54(2):69-75.
15. Singh M, Rishi S. Plasma acetylcholinesterase as a biomarker of
triazophos neurotoxicity in young and adult rats. Environ Toxicol
Pharmacol 2005;19(3):471-6.
16. Hollander H, Weigand W. Testing for acute intraperitoneal toxicity
in the male SPF wistar rats. Unpublished Report No. 77.0364 from
Hoechst AG, Pharma Development, Submitted to WHO by Aventis
CropScience, Frankfurt am Main, Germany; 1977. Available from
(http://sgh.org.sa/Portals/Articles/Occupational and Environmental
Health-Recognizing and Preventing Disease and Injury.pdf. [Last
accessed on 2013 Sep 18].
17. Hollander H, Weigand W. Acute oral toxicity to the male SPF-wistar
rat. Unpublished Report No. 77.0360 from Hoechst AG, Pharma
Research Toxicology, Submitted to WHO by Aventis Crop Science,
Frankfurt Main, Germany; 1977. (Available from http://sgh.org.sa/
Portals/Articles/Occupational and Environmental Health-Recognizing
and Preventing Disease and Injury.pdf. [Last accessed on 2013 Sep 18].
18. Available from: http://www.inchem.org/documents/jmpr/
jmpmono/2002pr14.htm. [Last accessed on 2013 Sep 18].
19. Ghosh MN. Fundamentals of Experimental Pharmacology. 2nd ed.
Calcutta, India: Scientific Book Agency; 1984. p. 153-8.
20. Turner R. Quantal responses calculation of the ED50. Screening Methods
in Pharmacology. New York: Academic Press; 1965. p. 61-3.
21. Randhawa MA. Calculation of LD50 values from the method of Miller
and Tainter, 1944. J Ayub Med Coll Abbottabad 2009;21(3):184-5.
22. Aprea C, Strambi M, Novelli MT, Lunghini L, Bozzi N. Biologic
monitoring of exposure to organophosphorus pesticides in 195 Italian
children. Environ Health Perspect 2000;108(6):521-5.
23. Barr DB, Bravo R, Weerasekera G, Caltabiano LM, Whitehead RD Jr,
Olsson AO, et al. Concentrations of dialkyl phosphate metabolites of
organophosphorus pesticides in the U.S. population. Environ Health
Perspect 2004;112(2):186-200.
24. Curl CL, Fenske RA, Elgethun K. Organophosphorus pesticide
exposure of urban and suburban preschool children with organic and
conventional diets. Environ Health Perspect 2003;111(3):377-82.
25. Kattavenkatesh R, Kumar N. Study the assessment of poisoning cases
in a rural tertiary care teaching hospital by a clinical pharmacist. Asian
J Pharm Clin Res 2012;5(2):138-41.
26. Heikal TM, Mossa AT, Rasoul MA, Marei GK. The ameliorating effect
of green tea extract against cyromazine and chlorpyrifos induced liver
toxicity in male rats. Asian J Pharm Clin Res 2013;6(1):48-55.
27. Brown LM, Blair A, Gibson R, Everett GD, Cantor KP, Schuman LM,
et al. Pesticide exposures and other agricultural risk factors for leukemia
among men in Iowa and Minnesota. Cancer Res 1990;50(20):6585-91.
28. Waddell BL, Zahm SH, Baris D, Weisenburger DD, Holmes F,
Burmeister LF, et al. Agricultural use of organophosphate pesticides
and the risk of non-Hodgkin’s lymphoma among male farmers
(United States). Cancer Causes Control 2001;12(6):509-17.
29. World Health Organization. Public Health Impact of Pesticides Used
in Agriculture. Geneva: WHO; 1990. Available from: http://whqlibdoc.
who.int/publications/1990/9241561394.pdf. [Last accessed on 2014
Aug 04].
30. Riviere JE. Dermal absorption/toxicity of organophosphates and
carbamates. In: Gupta RC, editor. Toxicology of Organophosphate and
Carbamate Compound. New York: Elsevier Academic Press; 2006.
31. Manna S, Bhattacharya D, Basak DK, Mandal TK. Single oral dose
toxicity of α-cypermethrin in rats. Indian J Pharmacol 2004;36(1):25-8.
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How to Cite
Chandra, M., J. Raj, T. D. Dogra, A. C. Rajvanshi, and A. Raina. “DETERMINATION OF MEDIAN LETHAL DOSE OF TRIAZOPHOS (TZ) WITH DMSO IN WISTAR RAT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 7, no. 4, Sept. 2014, pp. 64-67, https://innovareacademics.in/journals/index.php/ajpcr/article/view/1085.
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