IN VITRO ANTI-ACETYL CHOLINESTERASE AND ANTIOXIDANT ACTIVITY OF SELECTED MALAYSIAN PLANTS
Objective: The aim of this study is to discover and identify the potential plant extracts that could be vital against Alzheimerâ€™s disease (AD). It is a neurodegenerative disease affecting the brain. The most promising target for the symptomatic treatment and slowing of AD progression is cholinesterase inhibitors from plants.
Methods: To evaluate the antioxidant and anti-acetylcholinesterase (AChEI) activities leaves and roots were macerated and refluxed with ethanol extracts, from four medicinal plants, namely; C.asiatica, M.pudica, C.pumila, and L.camara. DPPH and Î²-carotene assays were used to determineÂ antioxidant activity;Â whereas Ellmanâ€™s colorimetric method was adopted to quantify AChEIÂ activity.
Results: The refluxed ethanol extract of M.pudica leaves exhibited the highest AChEIÂ activityÂ (IC50Â =Â 0.047Â mg/ml) and high antioxidant activityÂ (Î²-carotene; IC50Â =Â 0.14Â mg/ml and DPPH; IC50Â =Â 0.012 mg/ml).
Conclusion: Results reveal that all plant extracts studied possess anti-oxidant properties. Most potent extracts could be a lead to novel antioxidants and acetyl cholinesterase inhibitors, for the treatment of AD.
ÂKeywords; Alzheimerâ€™s disease (AD); Acetylcholinesterase activity; Antioxidant activity; Î²-carotene; DPPH; Medicinal plants.
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