GC MS Analysis and Antibacterial Activity of Myristica fragrans seed Extracts against Lower Respiratory tract pathogen Acinetobacter baumanii

  • Kumaresan Sundar Assistant Professor,Department of Microbiology, M.R.Government Arts College,Mannargudi,TamilNadu
  • T.P.Kumari Pushpa Rani
  • B. Vijayalakshmi Amma

Abstract

This study is destined to evaluate the antimicrobial activity of Nutmeg (Myristica fragrans Houtt) seed extract against the lower respiratory pathogen (Acinetobacter baumannii). The extract was subjected to phytochemical detection which confirms the presence of alkaloids, terpenoids, flavonoids, phenols and quinones, with the exception of Phlobatanins.  The seed extract was tested against sixteen isolates of Acinetobacter baumannii . The susceptibility of these isolates towards the seed extracts was compared with each other and the antibiotic (Meropenem) which was used a as a positive control. Results in the former showed that toulene, tetrahydrofuran and methanol extracts of the seed exhibited antibacterial activity against the bacterial isolates The bioactive components present in the methanol extract were also evaluated by GC-MS analysis and the analysis revealed the presence of thirteen compounds.The study concludes that the secondary metabolites present in the plant  can be an indispensible source of anitimicrobial compounds.

Keywords: Myristica frangrans, Acinetobacter baumannii, solvents, antibacterial activity

Author Biography

Kumaresan Sundar, Assistant Professor,Department of Microbiology, M.R.Government Arts College,Mannargudi,TamilNadu
Assistant Professor,Department of Microbiology, M.R.Government Arts College,Mannargudi,TamilNadu

References

References
[1] Zhang X. Traditional Medicine: It is Importance and Protection. In: S. Twarog and P. Kapoor, (eds), “Protecting and Promoting Traditional Knowledge. Part 1, New York: United Nations; pp: 3-6; 2004.
[2] Hinneburg, H.J.D. Dorman, R. Hiltunen. Food Chem., 97 (2006), pp. 122–129
[3] Y. Yano, M. Satomi, H. Oikawa. Int. J. Food Microbiol., 111 (2006), pp. 6–11

[4] Tajuddin, S. Ahmad, A. Latif, I.A. Qasmi, BMC Complement. Altern. Med., 3 (2003), pp. 6–10

[5] J.K. Grover, S. Khandkar, V. Vats, Y. Dhunnoo, D. Das. Methods Find. Exp. Clin. Pharmacol., 24 (2002), pp. 675–680

[6] O.A. Olajide, F.F. Ajayi, A.L. Ekhelar, S.O. Awe, J.M. Makinde, A.R.A. Alada, Phytother. Res., 13 (1999), pp. 344–345

[7] K. M. Abdurrasheed, C. Janardanan, J. Spices Aromatic Crops, 18(2), 108 (2009).
[8] ] Periyasamy A, Rajkumar, Mahalingam K., 2010. Phytochemical screening and antimicrobial activity from five Indian medicinal plants against human pathogens. Middle-East Journal of Scientific Research, 5(6): 477-482


[9] Ronald Hites A. Gas Chromatography Mass Spectroscopy: Handbook of Instrumental Techniques for Analytical Chemistry, 1997. p. 609-611.

[10] Sundar S K., Palavesam A and Parthipan B.,2008. Antimicrobial and phytochemical studies
of AM fungal treated selected medicinal plants of Kanyakumari District, Tamil nadu; College Sadhana Bloomers of Research; 1; 36-41.

[11] Harborne J.B, 1998. Phytochemical methods:A guide to modern technique of plant analysis, Champman and Hall, London

[12] Cuppucino and Shermann., 1996. Microbiology A Laboratory Manual; 6th Edition.


[13] Bauer A.N., Kirby W.M.M., Sherries J.C and Truck M., 1996. Antibiotic susceptibility testing by a standardized single disk method. Amer.J.Clin.Pathol: 45(4): 493-436.

[14] Gurumani. M J P Publications, An Introduction to Biostatistics. p-280, 2004

[15] Harborne J.B, 1998. Phytochemical methods: A guide to modern technique of plant
analysis, Champman and Hall, London

[16] Jukic.M . O. Politeo, M. Milos Croat. Chem. Acta, 79 (2006), pp. 209–214

[17] Chatterjee.S Z. Niaz, S. Gautam, S. Adhikari, P.S. Variyar, A. Sharma Food Chem., 101 (2007), p. 515–523
[18] Anish Deep Gupta, Vipin Kumar Bansal, Nishi Maithil.Chemistry, antioxidant and antimicrobial potential of nutmeg (Myristica fragrans Houtt).J . Genetic Eng.Biotech.Vol 11, (1), p 25-31

[19] Elizabeth Thomas, Aneesh T. , Della Grace Thomas, r. Anandan. GC-MS analysis of phytochemical compounds present in the rhizomes of Nervilia aragoana GAUD. Asian J Pharma Clinical Res. Vol 6, Suppl 3, 2013


[20] H.J.D. Dorman, S.J. Deans J. Essent. Oil Res., 16 (2004), pp. 145–150

[21] W.A. Konig, A. Kruger, D. Icheln, T. Runge J. High Resolut. Chromatogr., 15 (1992), pp. 184–189.

[22] M. Cristani, M. D’Arrigo, G. Mandalari, F. Castelli, M.G. Sarpietro, D. Micieli, V. Venuti, G. Bisignano, A. Saija, D. Trombetta. J. Agric. Food Chem., 55 (2007), pp. 6300–6308
[23] A. Ultee, E.P.W. Kets, E.J. Smid, Appl. Environ. Microbiol., 65 (1999), pp. 4606–4610


[24] A. Ultee, M.H.J. Bennink, R. Moezelaar. Appl. Environ. Microbiol., 68 (2002), pp. 1561–1568
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How to Cite
Sundar, K., T. Pushpa Rani, and B. V. Amma. “GC MS Analysis and Antibacterial Activity of Myristica Fragrans Seed Extracts Against Lower Respiratory Tract Pathogen Acinetobacter Baumanii”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 7, no. 3, July 2014, pp. 126-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/1182.
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