ANTIMICROBIAL EFFICACY OF ORCHID EXTRACTS AS POTENTIAL INHIBITORS OF ANTIBIOTIC RESISTANT STRAINS OF ESCHERICHIA COLI
Keywords:Aerides odorata, Acampe papilosa, Acampe ochracea, Escherichia coli, antimicrobial activity, orchids
The objective of the study is to find the bioactivity of few unexplored orchids against antibiotic resistance Escherichia coli (E. coli). Three orchid species namely Aerides odorata, Acampe papilosa & Acampe ochracea that are ethnic to North-East India were investigated for their antimicrobial efficacy against Kanamycin and Ampicillin resistant E. coli.
Plants were collected, identified following literature and college herbarium of Karimganj College, Karimganj and shade dried. Extracts were prepared in different solvents in increasing polarity and paper discs soaked in extracts were prepared. Disc plate method was employed to determine the antimicrobial activity of the orchid extracts. The zones of inhibition were measured and interpreted compared to that of available antibiotics.
Water extract of A. ordata showed 0.5 cm, 0.7 cm & 0.5 cm, A. papilosa showed 0.5 cm, 0.6 cm & 0.5 cm and A. ochracea showed 0.5 cm, 0.8 cm & 0.6 cm inhibition zone against antibiotic susceptible E. coli, ampicillin resistant E. coli & kanamycin resistant E. coli respectively.
The results suggest that all the three orchid species has good antimicrobial activity against the three strains of E. coli. Although, the work is a preliminary study on antimicrobial screening but the possible efficacy of the three ethnic orchid species opened a new area for antimicrobial drug design research.
Aerides odorata; Acampe papilosa; Acampe ochracea; Escherichia coli; antimicrobial activity; orchids.
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