IN-VITRO PROPAGATION OF AN ENDANGERED MEDICINAL PLANT PSORALEA CORYLIFOLIA LINN

Authors

  • priyanka pandey
  • Rakesh Mehta
  • Ravi Upadhyay

Abstract

Objective: Here, we established the protocol for plant regeneration of Psoralea corylifolia via In-vitro micropropagation.

Methods: The Apical meristems was used as the explants cultured on Murashige and Skoog (1962) medium (MS) supplemented different concentrations and combinations of plant growth regulators, 6-Benzylaminopurine (BAP), Kinetin (Kn), 1-Naphthaleneacetic acid (NAA) and B5 vitamins + 2 mg/ltr. Glycine (MBG).

Results: Highest Shoot regeneration (95%) results were obtained on MS medium containing BAP (12 µM) with NAA (2.0 µM) and Kn (3.0 µM) generating shoots (6.12 shoots). BAP 12 µM found to be best for shoot multiplication. All the micro-shoots produced normal roots within two weeks of culture on the basic MS medium supplemented with auxin, viz. IAA, IBA or NAA. Maximum of 95% shoots were rooted with an average of 6.8 roots per shoot and average length of 7.11cm on MS medium supplemented with 0.5 μM IBA. Plantlets were transferred to pots where they grew well, attained maturity and set viable seeds.

Conclusion: The micropropagation protocol reported here was characterized with a rapid proliferation of shoots, easy rooting of the micro-shoots and the plantlets were easily acclimatized to the external environment and undergoing normal physiological development.


References

Uniyal B. Utilization of medicinal plants by the rural women of Kulu, Himachal Pradesh, Ind J Trad Knowledge. 2003; 2(4): 366-370.

Shilandra KU, Yadav AS, Sharma AK, Rai AK, Raghuwanshi DK, Badkhane Y. The botany, chemistry, pharmacological and therapeutic application of Psoralea corylifolia Linn. A review, Int J Phytomed. 2010; 2(2):100-107.

Kaufman P, Duke P, Brielmann J, Boik H, Hoit J. A comparative survey of leguminous plants as a source of the isoflavones, genistein and daidzein: Implications for human nutrition and health. Alter Comp Med. 1997; 3: 7-12.

Hsu Y, Wu C, Chem J, Yang Y, Wang S. The presence of three isoflavonoid compounds in Psoralea corylifolia. J. Chromatographic Sci. 2001; 39: 441- 444.

Baskaran P, Jayabalan N.Effect of growth regulators on rapid micropropagation and psoralen production in Psoralea corylifolia L. Acta Physiol Plant. 2008; 30: 345–351.

Das P, Samantaray S, Roberts AV, Rout GR. In vitro somatic embryogenesis of Dalbergia sissoo Roxb. – A multipurpose timber yielding tree. Plant Cell Rep. 1997; 16: 578–582.

Gulati A, Jaiwal PK. Micropropagation of Dalbergia sissoo from nodal explants of mature trees. Biol Plant. 1996; 38: 169–175.

Cascado JP, Navarro MC, Utrilla MP, Martinez A, Jimenez J. Micropropagation of Santolina canescens Lagasca and in vitro volatiles production by shoot explants. Plant Cell Tissue Organ Cult. 2002; 69:147-153.

Faisal M, Anis M. Rapid in vitro propagation of Rauvolfia tetraphylla L.—An endangered medicinal plant. Physiol Mol Biol Plants. 2002; 8: 295-299.

Fraternale D, Giamperi L, Ricci D, Rocchi MBL. Micropropagation of Bupleurum fruticosum: The effect of triacontanol. Plant Cell Tissue Organ Cult. 2002; 69: 135- 140.

Martin KP. Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep. 2003; 21: 415-420.

Sharma N, Chandel KPS, Paul A. In vitro propagation of Gentiana kurroo: an indigenous threatened plant of medicinal importance. Plant Cell Tiss Org Cult. 1993; 34: 307-309.

Fracro F, Echeverrigaray S. Micropagation of Cunila galoides, a popular medicinal plant of South Brazil. Plant Cell Tiss Org Cult. 2001; 64: 1-4.

Shahzad A, Faisal M, Anis M. Micro propagation through excised root culture of Clitoria ternatea and comparison between in vitro–regenerated plants and seedlings. Ann Appl Biol. 2007; 150: 341-349.

Baskaran P, Jayabalan N. In vitro regeneration of Psoralea corylifolia L. through callus cultures. Plant Biotechnology. 2009; 26: 333–336.

Sato S, Newell C, Kolacz K, Tredo L, Finer J, Hinchee M. Stable transformation via particle bombardment in two different soybean regeneration system. Plant Cell Rep. 1993; 12: 408–413.

Evans DA, Sharp WR, Flick CE. In Plant Tissue Culture– Methods and Applications in Agriculture (ed. Thorpe, T. A.), New York: Academic Press; 1981. p. 45–113.

Thorpe TA. In Techniques and Applications of Plant Cell and Tissue Culture to Agriculture and Industry (eds Tomes, D. T. et al.), University of Guelph, Ontario, 1982. p. 115–138.

Kumari M. Evaluation of methanolic extracts of in vitro grown Tinospora cordifolia (willd) for antibacterial activities. Asian J Pharm Clin Res. 2012; 5(3) 172-175.

Sharma N, Patni V. Grewia tenax (Frosk.) Fiori.- A traditional medicinal plant with enormous economic prospectives. Asian J Pharm Clin Res. 2012; 5(3): 28-32

Published

2013-07-01

How to Cite

pandey, priyanka, R. Mehta, and R. Upadhyay. “IN-VITRO PROPAGATION OF AN ENDANGERED MEDICINAL PLANT PSORALEA CORYLIFOLIA LINN”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 3, July 2013, pp. 115-8, https://innovareacademics.in/journals/index.php/ajpcr/article/view/127.

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