IN-VITRO PROPAGATION OF AN ENDANGERED MEDICINAL PLANT PSORALEA CORYLIFOLIA LINN
Objective: Here, we established the protocol for plant regeneration of Psoralea corylifolia via In-vitro micropropagation.
Methods: The Apical meristems was used as the explants cultured on Murashige and Skoog (1962) medium (MS) supplemented different concentrations and combinations of plant growth regulators, 6-Benzylaminopurine (BAP), Kinetin (Kn), 1-Naphthaleneacetic acid (NAA) and B5 vitamins + 2 mg/ltr. Glycine (MBG).
Results: Highest Shoot regeneration (95%) results were obtained on MS medium containing BAP (12 ÂµM) with NAA (2.0 ÂµM) and Kn (3.0 ÂµM) generating shoots (6.12 shoots). BAP 12 ÂµM found to be best for shoot multiplication. All the micro-shoots produced normal roots within two weeks of culture on the basic MS medium supplemented with auxin, viz. IAA, IBA or NAA. Maximum of 95% shoots were rooted with an average of 6.8 roots per shoot and average length of 7.11cm on MS medium supplemented with 0.5 Î¼M IBA. Plantlets were transferred to pots where they grew well, attained maturity and set viable seeds.
Conclusion: The micropropagation protocol reported here was characterized with a rapid proliferation of shoots, easy rooting of the micro-shoots and the plantlets were easily acclimatized to the external environment and undergoing normal physiological development.
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