Qualitative and quantitative estimation of Î²-sitosterol from in vitro regenerated stem bark and callus of Helicteres isora L.
AbstractObjective: Qualitative and quantitative determination of Î²â€“sitosterol from nature grown and in vitro regenerated plantlets of Helicteres isora L. was carried out to assess the presence and relative amount of Î²â€“sitosterol in stem bark and callus.
Methods: Acetone extract was used for TLC using the mobile phase Toluene: Diethyl ether: Ethyl acetate: Acetic acid (80:10:10:0.2). HPLC detection and quantification was performed at a wavelength 196 nm.
Results: Maximum percentage of Î²â€“sitosterol was present in the in vitro regenerated bark which was found to be 0.18 %.
Conclusion: Present findings indicate that H. isora plants responded favourably to in vitro propagation thus producing higher amount of Î²â€“sitosterol as compared to naturally grown plants.
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How to Cite
Chawla, S., and Y. K. Bansal. “Qualitative and Quantitative Estimation of Î²-Sitosterol from in Vitro Regenerated Stem Bark and Callus of Helicteres Isora L.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 7, no. 3, July 2014, pp. 178-80, https://innovareacademics.in/journals/index.php/ajpcr/article/view/1305.
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