Qualitative and quantitative estimation of β-sitosterol from in vitro regenerated stem bark and callus of Helicteres isora L.

Abstract

Objective: Qualitative and quantitative determination of β–sitosterol from nature grown and in vitro regenerated plantlets of Helicteres isora L. was carried out to assess the presence and relative amount of β–sitosterol in stem bark and callus.
Methods: Acetone extract was used for TLC using the mobile phase Toluene: Diethyl ether: Ethyl acetate: Acetic acid (80:10:10:0.2). HPLC detection and quantification was performed at a wavelength 196 nm.
Results: Maximum percentage of β–sitosterol was present in the in vitro regenerated bark which was found to be 0.18 %.
Conclusion: Present findings indicate that H. isora plants responded favourably to in vitro propagation thus producing higher amount of β–sitosterol as compared to naturally grown plants.