• Prashanta Kr Deb Regional Institute of Pharmaceutical Science & Technology, Abhoynagar, Agartala–799 005, Tripura (W), India.
  • Ranjib Ghosh Dept. of Pharmacology, TMC & Dr. BRAM Teaching Hospital, Hapania, Agartala–799 014, Tripura (W), India.
  • Sankari Das Women’s College, Govt. of Tripura, Agartala – 799 001, Tripura (W), India.
  • Tejendra Bhakta Regional Institute of Pharmaceutical Science & Technology, Abhoynagar, Agartala–799 005, Tripura (W), India.


Objectives: The present study was aimed to investigation of the anthelmintic potential of crude MeOH extract, 50% EtOH and aqueous extract of the leaves of Acorus calamus on Indian earth-worm (Pheretima posthuma).

Methods: Three concentrations (25, 50, 100 mg/ml) of each extract were studied in activity which involved the determination of time of paralysis (vermifuge) and time of death (vermicidal) of the worms. Albendazole in same concentration as those of extract was included as standard reference and normal saline water with 1% CMC as control.

Results: All the extracts exhibited significant anthelmintic activity at a concentration of 100 mg/ml. Peak activity was exhibited by the MeOH extract at a concentration of 100 mg/ml.

Conclusion: Findings of the present investigations confirms that, the ethno-medicinal claim of anthelmintic activity of this plant is genuine.


Key words: Acorus calamus, Anthelmintic activity, Albendazole, Vermifuge, Vermicidal.


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Hossain E, Chandra G, Nandy AP, Mandal SC & Gupta JK: Anthelmintic effect of a methanol extract of leaves of Dregea volubilis on Paramphistomum explanatum. Parasitol Res 2012; 110: 809–814.

Tripathi KD. Essentials of Medical Pharmacology. 6th ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd. 2008. p. 808-810.

Kirtikar KR, Basu BD. Indian Medical plants. Vol. 4, 2nd ed. Dehradun: International Book Publication Distribution 1987. p. 2626-2628.

Chopra R.N., Chopra I.C., Handa K.L. and Kapur L.D: Indigenous Drugs of India, Calcutta, UN Dhar & Sons Ltd. 1950.

Divya G, Gajalakshmi S, Mythili S and Sathiavelu A: Pharmacological Activities of Acorus calamus: A Review. AJBPR 2011; 1(2): 57-64.

Raja AE, Vijayalakshmi M and Devalarao G: Acorus calamus Linn.: Chemistry and Biology. Research J. Pharm. and Tech 2009; 2 (2): 256-261.

Kokate CK, Purohit AP, Gokhale SB: Text Book of Pharmacognosy. 41st ed. Pune: Nirali Prakashani; 2008.

Chatterjee KD. Patasitology, Protozoology and Helminthology. Calcutta: Guha Ray Sree Saraswaty Pres Ltd.; 1967. p. 168-169.

Ajaiyeoba EO, Onocha PA and Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extract. Pharm Bio 2001; 39: 217†220.

Kumar T, Alexander A, Dewangan D, Nagori K.: Anthelmintic Activity of the Whole Plant of Bauhinia purpurea (linn.). Asian J Pharm Clin Res 2011; 4(3): 110-111.

Kosalge SB, Fursule RA: Investigation of In vitro Anthelmintic activity of Thespesia Lampas (Cav.). Asian J Pharm Clin Res 2009; 2(2): 69-71.

Chandan HS, Tapas AR, Sakarkar DM: Anthelmintic activity of extracts of Coriandrum sativum Linn. in Indian earthworm. Int. J Phytomedicine 2011; 3: 36-40.



How to Cite

Deb, P. K., R. Ghosh, S. Das, and T. Bhakta. “IN-VITRO ANTHELMINTIC ACTIVITY OF ACORUS CALAMUS LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 3, July 2013, pp. 135-7,