A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS


Sumathy Rengarajan, Vijayalakshmi Melanathuru, Deecaraman Munuswamy, Sankaranarayanan Sundaram, Saravanan Thiruverkadu Selvaraj

Abstract


Objective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of four
different Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).

Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standard
procedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.

Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,
and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activity
against Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 μl/ml and also
showed the maximum inhibitory activity at the highest concentration (20 μl/ml) than the lowest concentration (5 μl/ml) against Gram-negative
bacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanol
extracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with different
Rf values.

Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterial
activity and can be used as an antibacterial agent against bacterial diseases.

Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.


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References


Alabe PI, Irobi ON. Antibacterial activities of crude extract of Acalypha wilkesinan from Manna Nigeria. J Ethanopharmacol 1993;39:235-6.

Gurinder JK, Daljit SA. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complement Alter Med 2009;9:30.

Franklin TJ, Snow CA. Biochemistry of Antimicrobial Action. 4th ed. New York: Chapman and Hall; 1989. p. 134-55.

Prescott L, Harley J, Klein DA. Microbiology. 5th ed. London: McGraw- Hill; 2002. p. 820-950.

van der Watt E, Pretorius JC. Purification and identification of active antibacterial components in Carpobrotus edulis L. J Ethnopharmacol 2001;76(1):87-91.

Sharif MD, Banik GR. Status and utilization of medicinal plants in Rangamati of Bangladesh. Res J Agric Biol Sci 2006;2(6):268-73.

Doughari JH, El-mahmood AM, Manzara S. Studies on the antibacterial activity of root extracts of Carica papaya L. Afr J Microbiol Res 2007;1(3):37-41.

Maganha EG, Da costa HR, Rosa RM, Pegas HO, De Paula RA, Saffi J. Pharmacological evidences for the extracts and secondary metabolites from plants of the genus Hibiscus. Food Chem 2009;118(1):1-10.

Gauthaman KK, Saleem MT, Thanislas PT, Prabhu VV, Krishnamoorthy KK, Devaraj NS, et al. Cardioprotective effect of the Hibiscus rosa sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat. BMC Complement Altern Med 2006;6:32.

Nade VS, Kanhere SV, Kawale LA, Yadav AV. Cognitive enhancing and antioxidant activity of ethyl acetate soluble fraction of the methanol extract of Hibiscus rosa sinensis in scopolamine-induced amnesia. Indian J Pharmacol 2011;43(2):137-42.

Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed., Vol. III. Dehradun: International Book Distributors; 1987. p. 867-8.

Gupta S, Sharma SB, Prabhu KM, Bansal SK. Protective role of Cassia auriculata Leaf extract on hyperglycemia-induced oxidative stress and its safety evaluation. Indian J Biochem Biophys 2009;46(5):371-7.

Das S, Sarma G, Barman S. Hepatoprotective activity of aqueous extract of fruit pulp of Cassia fistula (AFCF) against carbon tetrachloride (CCL4) induced liver damage in albino rats. J Clin Diagn Res 2008;2(5):1133-8.

Deore SL, Khadabadi SS, Kamdi KS, Ingle VP, Kawalkar NG, Sawarkar PS, et al. In vitro anthelmintic activity of Cassia tora. Int J Chem Tech Res 2009;1(2):177-9.

Duraipandiyan V, Ignacimuthu S. Antibacterial and antifungal activity of Cassia fistula L.: An ethnomedicinal plant. J Ethnopharmacol 2007;112(3):590-4.

Kaur G, Alam MS, Jabbar Z, Javed K, Athar M. Evaluation of antioxidant activity of Cassia siamea flowers. J Ethnopharmacol 2006;108(3):340-8.

Kirthkar KR, Basu BD. Medicinal Plants of India. Vol. I. New Delhi: CSIR; 1965. p. 518-20.

Samy RP, Ignacimuthu S. Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats of India. J Ethnopharmacol 2000;69(1):63-71.

Parkas SK. Effects of Herbal Extracts Towards Microbicidal Activity Against Pathogenic Escherichia coli in Poultry. Int J Poult Sci 2006;5(3):259-61.

Singh K, Tafida GM. Antibacterial activity of Moringa oleifera (LAM) leaves extract against some selected bacteria. Int J Pharm Pharm Sci 2014;6(9):52-4.

Pal SK, Mukherjee PK, Saha BP. Studies on the antiulcer activity of Moringa oleifera leaf extract on gastric ulcer models in rats. Phytother Res 1995;9(6):463-5.

Cáceres A, Saravia A, Rizzo S, Zabala L, De Leon E, Nave F. Pharmacologic properties of Moringa oleifera. 2: Screening

for antispasmodic, anti-inflammatory and diuretic activity. J Ethnopharmacol 1992;36(3):233-7.

Morton JF. The horse radish tree: M. Pterigosperma (Moringacea). A boon to arid lands. Econ Bot 1991;45(3):318-33.

Dahot MU. Vitamin contents of flowers and seeds of Moringa oleifera. Pak J Biochem 1988;21:1-24.

Mehta K, Balaraman R, Amin AH, Bafna PA, Gulati OD. Effect of fruits of Moringa oleifera on the lipid profile of normal and hypercholesterolaemic rabbits. J Ethnopharmacol 2003;86(2-3):191-5.

Ruckmani K, Kavimani S, Anandan R, Jaykar B. Effect of Moringa oleifera Lam on paracetamol – Induced hepatoxicity. Indian J Pharm Sci 1998;60(1):33-5.

Nickon F, Saud ZA, Rehman MH, Haque ME. In vitro antimicrobial activity of the compound isolated from chloroform extract of Moringa oleifera Lam. Pak J Biol Sci 2003;6(22):1888-90.

Naqvi SA, Khan MS, Vohora SB. Antibacterial, antifungal, and anthelmintic investigations of Indian medicinal plants. Fitoterapia 1991;62(3):221-8.

Lad V, Frawley D. The Yoga of Herbs. Santa Fe, NM: Lotus Press; 1986. p. 135-6.

Cáceres A, Girón LM, Alvarado SR, Torres MF. Screening of antimicrobial activity of plants popularly used in Guatemala for the treatment of dermatomucosal diseases. J Ethnopharmacol 1987;20(3):223-37.

Harbone JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hill; 1998. p. 279.

Rios JL, Recio MC, Villar A. Screening methods for natural products with antimicrobial activity: A review of the literature. J Ethnopharmacol 1988;23(2-3):127-49.

Akinpelu DA, Kolawole DO. Phytochemical and antimicrobial activity of leaf extract of Piliostigma thonningii (Schum.). Sci Focus J 2004;7:64-70.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. New Delhi, India: Springer Pvt. Ltd.; 1998.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.

Swathi S. Phytochemical screening and TLC studies of Moringa oleifera: Their antibacterial and antioxidant activities. Int J Curr Pharm Res 2016;8(1):46-9.

de Boer HJ, Kool A, Broberg A, Mziray WR, Hedberg I, Levenfors JJ. Anti-fungal and anti-bacterial activity of some herbal remedies from Tanzania. J Ethnopharmacol 2005;96(3):461-9.

Singh DV, Gupta MM, Kumar TR, Saikia D, Khanuja SP. Antibacterial principles from the bark of Terminalia arjuna. Curr Sci 2008;94(1):27-9.

Nair R, Kalariya T, Sumitra C. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol Sci 2005;29:41-7.




About this article

Title

A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

DOI

10.22159/ajpcr.2016.v9i5.13476

Date

01-09-2016

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 9 Issue 5 September 2016 Page: 259-263

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Sumathy Rengarajan
DR.MGR Educational and research institute
India

Vijayalakshmi Melanathuru

Deecaraman Munuswamy

Sankaranarayanan Sundaram

Saravanan Thiruverkadu Selvaraj


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