IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING OF PATHOGENIC MICRO-ORGANISM FROM DENTAL PATIENTS

  • Sakthivel M Periyar University,Salem, Tamilnadu
  • Ayyasamy Pm
  • Arvind Prasanth D

Abstract

ABSTRACT
Objective: To isolate and identify aerobic microbes present in the periodontal infected patients and to evaluate the choice of antibiotics in the
management of periodontal diseases.
Methods: In this study, these patients have not been treated previously for their conditions. An informed consent was obtained from these patients
before collection of an oral swab. This study was approved by the Institutional Ethical Committee. The details of the patient's age, sex, and clinical
details were recorded on a per forma meant for this study. The following methodologies were adopted for the isolation and identification of the microorganisms
from
these cases.
Results: In this study out of 50 oral samples, culture positivity was recorded in 43 (86%) cases and no growth in 7 (14%) cases. Antibiotic susceptibility
test using to identified as resistant, sensitive, intermediate of pathogenicity of oral microbes. Such antibiotics were methicillin, ceftazidime,
clindamycin, amikacin, cloxacillin, and cefotaxime. This study should be kept in mind when a local application of antibacterial compounds is used in
the therapy of periodontal disease.
Conclusion: This study highlights the different organisms involved in the different types of dental infections. The antibiotic pattern shown in this work
will be a guide to the clinician in the selection of proper antibiotics for the treatment of these infections. Hence in this study, the limitations were time
and the number of patients. For better outcomes, a larger study population for a longer period of time should be undertaken to know the bacteriology
and to the select the effective drugs of choice for dental infections. A comparative study of bacteriology and mycology and its antimicrobial property
would be very fruitful in the future.
Keywords: Dental, Periodontal, Bacteria, Antibiotics.

References

REFERENCES
1. Emes Y, Buket A, Serhat Y. On the evolution of human jaws and teeth:
A review. Bull Int Assoc Paleodont 2011;5(1):37-47.
2. Metivier A, Bland K. Dental Anatomy: A Review. Crest Oral-B
Continuing Education Course. 2013. p. 1-23. Available from: http://
www.dentalcare.com. [Last accessed on 2013 Apr 23].
3. Kuramitsu HK, He X, Lux R, Anderson MH, Shi W. Interspecies
interactions within oral microbial communities. Microbiol Mol Biol
Rev 2007;71(4):653-70.
4. Gonzales-Marin C, Spratt DA, Millar MR, Simmonds M, Kempley ST,
Allaker RP. Levels of periodontal pathogens in neonatal gastric
aspirates and possible maternal sites of origin. Mol Oral Microbiol
2011;26(5):277-90.
5. Karnure M, Munot N. Review on conventional and novel techniques for
treatment of alveolar osteitis. Asian J Pharm Clin Res 2013;6(3):13-7.
6. Bastos JA, Diniz CG, Bastos MG, Vilela EM, Silva VL, Chaoubah A,
et al. Identification of periodontal pathogens and severity of
periodontitis in patients with and without chronic kidney disease. Arch
Oral Biol 2011;56(8):804-11.
7. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility
testing by a standardized single disk method. Am J Clin Pathol
1966;45(4):493-6.
8. Weyrich LS, Dobney K, Cooper A. Ancient DNA analysis of dental
calculus. J Hum Evol 2015;79:119-24.
9. Janatová T, Najmanová L, Neubauerová L, Kyselková M, Novotná G,
Spízek J, et al. Changes in the incidence of periodontal pathogens
during long-term monitoring and after application of antibacterial
drugs. Folia Microbiol (Praha) 2009;54(5):429-35.
10. Prasad KV, Anupama I, Rao N, Sreenivasan PK, Subramanyam R,
Kulkarni RD. The effect of the dentifrice on gingivitis and dental
plaque: A 6-week clinical study in India. J Indian Assoc Public Health
Dent 2015;13:4-10.
11. Chukhlovin AB, Solovyova AM, Matelo SK, Kobiyasova IV,
Morosova EB, Hokhlacheva AV, et al. Bacterial markers of periodontal
diseases and their practical significance in dentistry. Bull Exp Biol Med
2007;144(4):546-50.
12. Santhosh-Kumar MP, Harshini AK. Knowledge and awareness about
oral cancer among undergraduate dental students. Asian J Pharm Clin
Res 2016;9(4):1-3.
13. Supranoto SC, Slot DE, Addy M, Van der Weijden GA. The effect
of chlorhexidine dentifrice or gel versus chlorhexidine mouthwash
on plaque, gingivitis, bleeding and tooth discoloration: A systematic
review. Int J Dent Hyg 2015;13(2):83-92.
14. Antipa C, Dascalu L, Chifiriuc MC, Lazar V, Bleotu CC, Ruta SM.
Isolation, identification and antibiotic susceptibility profiles in bacterial
strains isolated from periodontal lesions. Ann Biol Res 2014;5(3):22-6.
15. He XS, Shi WY. Oral microbiology: Past, present and future. Int J Oral
Sci 2009;1(2):47-58.
16. Ranganathan K, Magesh KT, Kumarasamy N, Solomon S,
Visvanathan R, Johnson NW. Greater severity and extent of periodontal
breakdown in 136 South Indian human immunodeficiency virus
seropositive patients than in normal controls: A comparative study
using community periodontal index of treatment needs. India J Dent
Res 2007;18(2):55-9.
17. Saini S, Aparna, Gupta N, Mahajan A, Arora DR. Microbial flora in
orodental infections. Indian J Med Microbiol 2003;21(2):111-4.
18. Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation
of yeasts and enteric bacteria in root-filled teeth with chronic apical
periodontitis. Int Endod J 2001;34(6):429-34.
2
Statistics
310 Views | 364 Downloads
Citatons
How to Cite
M, S., A. Pm, and A. Prasanth D. “IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING OF PATHOGENIC MICRO-ORGANISM FROM DENTAL PATIENTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 8, Oct. 2016, pp. 226-30, doi:10.22159/ajpcr.2016.v9s2.13668.
Section
Original Article(s)