HPLC METHOD FOR QUANTIFICATION OF ELLAGIC ACID IN IN VIVO AND IN VITRO PLANT PARTS OF OROXYLUM INDICUM (L.) VENT.
Objective: The present study was performed to investigate the comparative analysis of ellagic acid content in in vivo (stem, leaf and root) and in vitro (callus, in vitro developed root and shoot) samples of Oroxylum indicum (L.) Vent. an important medicinal plant.
Methods: For in vitro culture, seedling explants were inoculated on MS (Murashige and Skoog's, 1962) medium, supplemented with BAP and Kn alone and combination with IAA. Analytical method HPLC was developed for the quantification of ellagic acid in in vivo and in vitro samples.
Result: BAP combination with IAA was best for shoot multiplication, BAP with 2,4-D were used for callus proliferation. HPLC analysis of these extracts revealed that the quantity of ellagic acid present in both the samples but maximum ellagic acid content was obtained in leaves among all plant parts.
Conclusion: Hence, the present study showed the in vivo as well as in vitro samples contain ellagic acid. That can be used for its large scale production in future.
Â Key-words: Oroxylum indicum, ellagic acid, HPLC
Warrier PK, Nambiar VP, editors. Indian Medicinal Plants. Vol. 4.
Madras, India: Orient Longman Ltd.; 1995. p. 186-90.
2. Sankara S, Nair AG. Flavanoid of stem bark of Oroxylum indicum. Curr
3. Sankara S, Nair AG. Flavonoids from the leaves of Oroxylum indicum
and Pajanelia longifolia. Phytochemistry 1972b;11:439-40.
4. Vasanth S, Natarajan M, Sundaresan R, Rao RB, Kundu AB. Ellagic
acid from Oroxylum indicum Vent. Indian Drugs 1991;28(11):507-9.
5. Hong T, Jin GB, Cho S, Cyong JC. Evaluation of the anti-inflammatory
effect of baicalein on dextran sulfate sodium-induced colitis in mice.
Planta Med 2002;68(3):268-71.
6. Khennouf S, Benabdallah H, Gharzouli K, Amira S, Ito H, Kim TH,
et al. Effect of tannins from Quercus suber and Quercus coccifera
leaves on ethanol-induced gastric lesions in mice. J Agric Food Chem
7. Ng TB, Liu F, Wang ZT. Antioxidative activity of natural products from
plants. Life Sci 2000;66(8):709-23.
8. Niedworok J, Jankowstia B, Kowalczy E, Okroj W. A comparative
investigation of hepatoprotective effects of baicalein and sylimarol.
Herba Pol 1999;45:199-05.
9. Chiang LC, Ng LT, Chiang W, Chang MY, Lin CC. Immunomodulatory
activities of flavonoids, monoterpenoids, triterpenoids, iridoid
glycosides and phenolic compounds of Plantago species. Planta Med
10. Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R,
Popov S. Antibacterial, antifungal and antiviral activity of propolis of
different geographic origin. J Ethnopharmacol 1999;64(3):235-40.
11. Tahara S, Hashihidoka Y, Mizutani J. Flavonoids as medicines. Agric
Biol Chem 1987;51:1039-45.
12. Iqbal PJ. Phytochemical screening of certain plant species of Agra city.
J Drug Deliv Ther 2012;2(4):135-8.
13. Maitreyi Z, Khandhar A, Jain S. Quantification of baicalein, chrysin,
biochanin-a and ellagic acid in root bark of Oroxylum indicum by
RP-HPLC with UV Detection. Eur J Anal Chem 2008;3(2):245-57.
14. Rami E, Patel I. Quantitative analysis of total phenols and flavonoids
in in vivo and in vitro samples of Oroxylum indicum (L.) Vent. Asian J
Pharm Clin Res 2015;8(2):202-4.
15. Jadhav P, Laddha K. Estimation of gallic acid and ellagic acid from
Terminalia chebula Retz. Indian Drugs 2004;41(4):200-6.
16. Whitley AC, Stoner GD, Darby MV, Walle T. Intestinal epithelial cell
accumulation of the cancer preventive polyphenol ellagic acid - extensive
binding to protein and DNA. Biochem Pharmacol 2003;66(6):907-15.
17. Hari Babu T, Manjulatha K, Suresh Kumar G, Hymavathi A, Tiwari AK,
Purohit M, et al. Gastroprotective flavonoid constituents from Oroxylum
indicum Vent. Bioorg Med Chem Lett 2010;20(1):117-20.
18. Mostafa HA, EL-Bakry AA, Alam EA. Evaluation of antibacterial and
antioxidant activities of different plant parts of Rumex vesicarius L.
(Polygonaceae). Int J Pharm Pharm 2011;3(2):109-18.
19. Arya D, Patni V, Kant U. In vitro propagation and quercetin
quantification in callus cultures of Rasna (Pluchea lanceolata Oliver &
Hiern). Indian J Biotechnol 2008;7(3):383-7.
20. Goswami A, Reddi A. Antimicrobial activity of flavonoids of
medicinally important plant Cassia angustifolia in vivo and in vitro.
J Phytol Res 2004;17:179-81.
21. Sharma N, Patni V. Comparative analysis of total flavonoids, quercetin
content and antioxidant activity of in vivo and in vitro plant parts of
Grewia asiatica Mast. Int J Pharm Pharm Sci 2013;5(2):464-9.
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