• Sriram Prasath g *Dr. G. Sriram PrasathAssistant ProfessorPG and Research Department of Biochemistry, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam Chennai 600 106




Solanum nigrum is a paramount in medicinal perspective and belongs to family Solanacea. From different parts of the plant significant pharmacological and biological activities have been reported previously.  In the present study, the ethanolic extract of leaves was investigated for phytochemical analysis, vitamin and mineral content. Phytochemical analysis of the leaves revealed the presence of phenols, alkaloids, flavonoids, glycosides, saponins, tannins, phytosterols and triterpenoides. The leaves extract was found to contain appreciable amounts of flavonoids and phenols. The extract showed the presence of vitamins such as ascorbic acid, folic acid, and niacinamide. It has been found that the leaves of Solanum nigrum is rich in minerals like copper, magnesium, manganese, vanadium, chromium, calcium, zinc, sodium, and potassium. The antioxidant (free radical scavenging) activity of the extract was determined against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ABTS, Nitric oxide(NO) and Superoxide Scavenging assays. Solanum nigrum leaves extract was found to be antioxidant in nature which is evident from DPPH, ABTS, NO, Superoxide radical scavenging assays. These findings suggest that Solanum nigrum possess biologically important phytoconstituents, significant amounts of vitamins and minerals. IN addition, S. nigrum leaves extract exert free radical scavenging potential in vitro.

Key words: Medicinal plants; Solanum nigrum leaves; phytoconstiuents;Antioxidant.



Author Biography

Sriram Prasath g, *Dr. G. Sriram PrasathAssistant ProfessorPG and Research Department of Biochemistry, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam Chennai 600 106

Department of Biochemistry,

PG and Research Department of Biochemistry,

Chennai 600 106



Seth SD, Sharma B. Medicinal plants in India. Indian J Med Res


Rani P, Khullar N. Antimicrobial evaluation of some medicinal plants

for their anti-enteric potential against multidrug resistant Salmonella

typhi. Phytother Res 2004;18(8):670-3.

Zakaria ZA, Gopalan HK, Zainal H, Mohd Pojan NH, Morsid NA,

Aris A, et al. Antinociceptive, anti-inflammatory and antipyretic effects

of Solanum nigrum chloroform extract in animal models. Yakugaku

Zasshi 2006;126(11):1171-8.

Acharya E, Pokhrel B. Ethno - Medicinal plants used by Bantar of

Bhaudaha, Morang, Nepal. Our Nat 2006;4:96-103.

Lee SJ, Lim KT. Antioxidative effects of glycoprotein isolated from

Solanum nigrum Linne on oxygen radicals and its cytotoxic effects on

the MCF-7 cell. J Food Sci 2003;68:466-70.

Raju K, Anbuganapathi G, Gokulakrishnan V, Rajkapoor B, Jayakar B,

Manian S. Effect of dried fruits of Solanum nigrum LINN against CCl4-

induced hepatic damage in rats. Biol Pharm Bull 2003;26(11):1618-9.

Li J, Li Q, Feng T, Zhang T, Li K, Zhao R, et al. Antitumor activity

of crude polysaccharides isolated from Solanum nigrum Linne on U14

cervical carcinoma bearing mice. Phytother Res 2007;21(9):832-40.

Jainu M, Devi CS. Antioxidant effect of methanolic extract of Solanum

nigrum berries on aspirin induced gastric mucosal injury. Indian J Clin

Biochem 2004;19(1):57-61.

Bhatia N, Maiti PP, Kumar A, Tuli A, Ara T, Khan MU. Evaluation

of cardio protective activity of methanolic extract of Solanum nigrum

Linn. in rats. Int J Drug Dev Res 2011;3(3):139-47.

Ali NS, Singh K, Khan MI, Rani S. Protective effect of ethanolic

extracts of Solanum nigrum on the blood sugar of albino rats. Int J

Pharm Sci Res 2010;1(9):97-9.

Ravi V, Saleem TS, Maiti PP, Gauthaman K, Ramamurthy J.

Phytochemical and pharmacological evaluation of Solanum nigrum

Linn. Afr J Pharm Pharmacol 2009;3(9):454-7.

Harborne JB. Methods of extraction and isolation. In: Phytochemical

Methods. London: Chapman and Hall; 1998. p. 60-6.

Kokate CK. Practical Pharmacognosy. New Delhi, India: Vallabh

Prakashan; 2005. p. 107-11.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total

phenols and other oxidation substrates and antioxidants by means of

Folin-Ciocalteu reagent. Method Enzymol 1999;299:152-78.15. Kumazawa S, Taniguchi M, Suzuki Y, Shimura M, Kwon MS,

Nakayama T. Antioxidant activity of polyphenols in carob pods. J Agric

Food Chem 2002;50(2):373-7.

Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M,

et al. Phenolic compounds and antioxidant activities of buckwheat

(Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol


Brand-Williams W, Cuvelier ME, Berset C. Use of a free-radical method

to evaluate antioxidant activity. Food Sci Technol Leb 1995;28:25-30.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C.

Antioxidant activity applying an improved ABTS radical cation

decolorization assay. Free Radic Biol Med 1999;26:1231-7.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxidescavenging

properties of Ginkgo biloba extract EGb 761. Biochem

Biophys Res Commun 1994;201(2):748-55.

Fontana M, Mosca L, Rosei MA. Interaction of enkephalins with

oxyradicals. Biochem Pharmacol 2001;61:1253-7.

Devi S, Singh R. Antidiabetic activity of methanolic extract of Nepeta

hindostana herb in streptozotocin induced diabetes in rats. Int J Pharm

Pharm Sci 2016;8(7):330-5.

Huffman MA. Animal self-medication and ethno-medicine: Exploration

and exploitation of the medicinal properties of plants. Proc Nutr Soc


Craig W, Beck L. Phytochemicals: Health protective effects. Can J Diet

Pract Res 1999;60(2):78-84.

Amir M, Kumar S. Possible industrial application of genus solanum in

twenty first century - A review. J Sci Ind Res 2004;63:116-24.

Juneja D, Shrivastava PN, Guha MK, Saxena RC. Preliminary

phytochemical screening of some folklore medicinal plants for their

anti-inflammatory activity. Pharmacogn Mag 2007;3:11.

Kale MA, Bindu SM, Khadkikar P. Role of antioxidants and nutrition in

oxidative stress: A review. Int J Appl Pharm 2015;7(1):1-4.

Ruba AA, Mohan VR. In vitro antioxidant potential of whole plant of

Andrographis echioides (L.) nees (Acanthaceae). Int J Pharm Pharm

Sci 2016;8(6):70-5.

Shibuki K, Okada D. Endogenous nitric oxide release required

for long-term synaptic depression in the cerebellum. Nature


Bredt DS, Snyder SH. Nitric oxide: A physiologic messenger molecule.

Annu Rev Biochem 1994;63:175-95.

Moncada S, Palmer RM, Higgs EA. Nitric oxide: Physiology,

pathophysiology, and pharmacology. Pharmacol Rev 1991;43(2):109-42.

Dawson TM, Dawson VL, Snyder SH. A novel neuronal messenger

molecule in brain: The free radical, nitric oxide. Ann Neurol




How to Cite

Prasath g, S., ARCHANA PRAKASH, SRINIVASAN PT, and SUBRAMANIAN S. “STUDIES ON THE PHYTOCHEMICAL SCREENING AND FREE RADICAL SCAVENGING POTENTIALS OF SOLANUM NIGRUM LEAVES EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 6, Nov. 2016, pp. 316-21, doi:10.22159/ajpcr.2016.v9i6.14506.



Original Article(s)