• Bhumika Yogi Department of Pharmaceutical Chemistry, Hygia Institute of Pharmaceutical Education and Research, Lucknow-206020
  • Ashutosh Mishra


Objective: To investigate the hepatoprotective activity of aqueous methanolic extract of polyherbal formulation and individual extracts of Calotropis procera, Gymnema sylvestre, and Lawsonia inermis leaves.

Methods: The hepatoprotective activity of the formulation and extracts were assessed on the basis of histopathological changes in liver of carbon tetrachloride-induced hepatotoxic rats.

Results: The result of present studies strongly indicates that the hepatoprotective property of polyherbal formulation as evidenced by less damaged hepatocytes cells.

Conclusion: Polyherbal formulation revealed excellent hepatoprotective profile as compared to individual plants due to synergistic effect produced by different phytochemicals present in the formulation.

Keywords: Calotropis procera, Gymnema sylvestre, Lawsonia inermis, Carbon tetrachloride.

Author Biography

Bhumika Yogi, Department of Pharmaceutical Chemistry, Hygia Institute of Pharmaceutical Education and Research, Lucknow-206020

Department of Pharmaceutical Chemistry, Hygia Institute of Pharmaceutical Education and Research, Lucknow-206020


1. Petri WA. Antimicrobial agents. In: Gilman AG, Hardman JG, Limbird LE, editors. The Pharmacological Basis of Therapeutics. 10th ed. New York: McGraw-Hill Publication Corporation; 2001.p. 1189-93.
2. Mossa JS, Tariq M, Mohsin A, Ageel AM, Al-Yahya MA, Al-Said MS, et al. Pharmacological studies on aerial parts of Calotropis procera. Am J Chin Med 1991;19(3-4):223-31.
3. Yogi B, Murti Y, Pathak D. Pharmacognostical standardization of leaves of Calotropis procera (Ait) R. Br. (Asclepiadaceae). Int J Ayurveda Res 2010;1(1):14-7.
4. Kumar S, Dewan S, Sangraula H, Kumar VL. Anti-diarrhoeal activity of the latex of Calotropis procera. J Ethnopharmacol 2001;76(1):115-8.
5. Roy S, Sehgal R, Padhy BM, Kumar VL. Antioxidant and protective effect of latex of Calotropis procera against alloxan-induced diabetes in rats. J Ethnopharmacol 2005;102(3):470-3.
6. Choedon T, Mathan G, Arya S, Kumar VL, Kumar V. Anticancer and cytotoxic properties of the latex of Calotropis procera in a transgenic mouse model of hepatocellular carcinoma. World J Gastroenterol 2006;12:2517-22.
7. Dewan S, Sangraula H, Kumar VL. Preliminary studies on the analgesic activity of latex of Calotropris procera. J Ethnopharmacol 2000;73(1-2):307-11.
8. Mathur R, Gupta SK, Mathur SR, Velpandian T. Anti-tumor studies with extracts of Calotropis procera (Ait.) R.Br. root employing Hep2 cells and their possible mechanism of action. Indian J Exp Biol 2009;47:343-8.
9. Basu A, Chaudhuri AK. Preliminary studies on the antiinflammatory and analgesic activities of Calotropis procera root extract. J Ethnopharmacol 1991;31(3):319-24.
10. Kuta F. Antigungal effect of Calotropis procera stem bark on epidermophytonosum and trichophtongypseum. Afr J Biotechnol 2008;7(13):2116-8.
11. Ahirwa L, Singh S. Screening of methanolic extract of Gymnema sylvestre R. Br. Leaves for antioxidant potential. Int J Pharm Sci Rev Res 2013;19(1):87-91.
12. Liu HM, Kiuchi F, Tsuda Y. Isolation and structure elucidation of gymnemic acids, antisweet principles of Gymnema sylvestre. Chem Pharm Bull (Tokyo) 1992;40(6):1366-75.
13. Najafi S, Deokule SS. Studies on Gymnema sylvestre - A medicinally important plant of the family Asclepiadaceae. Trakia J Sci 2011;9:26-32.
14. Kanetkar P, Singhal R, Kamat M. Gymnema sylvestre: A memoir. J Clin
Biochem Nutr 2007;41(2):77-81.
15. Daisy P, Eliza J, Mohamed Farook KA. A novel dihydroxy gymnemic triacetate isolated from Gymnema sylvestre possessing normoglycemic and hypolipidemic activity on STZ-induced diabetic rats. J Ethnopharmacol 2009;126(2):339-44.
16. Reddy S, Gopal G, Sit G. In vitro multiplication of Gymnema sylvestre
R Br: An important medicinal plant. Curr Sci 2004;10:1-4.
17. Khanna GV, Kannabiran K. Anticancer-cytotoxic activity of saponins isolated from the leaves of Gymnema sylvestre and Eclipta prostrata on HeLa cells. Int J
Green Pharm 2009;3(3):227-9.
18. Imam H, Mahbub NU, Khan MF, Hana HK, Sarker MM. Alpha amylase enzyme inhibitory and anti-inflammatory effect of Lawsonia inermis. Pak J Biol Sci 2013;16(23):1796-800.
19. Devendra KB, Tiravenkata RS, Singh R. Xanthones from Lawsonia inermis. Phytochemistry 1977;16:1616-7.
20. Aiferah MA. Toxicity induced histological changes in selected organs of male (wistar) rats by Lawsonia inermis leaf extract. Eur J Med Plants 2012;2(2):151-8.
21. Mikhaeil BR, Badria FA, Maatooq GT, Amer MM. Antioxidant and immunomodulatory constituents of henna leaves. Z Natureforschung C 2004;59(7-8):466-8.
22. Hsouna AB, Trigui M, Culioli G, Blache Y, Jaoua S. Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure and antioxidative capacity. Food Chem 2011;125:193-200.
23. Kirkland D, Marzin D. An assessment of the genotoxicity of 2-hydroxy- 1,4-naphthoquinone, the natural dye ingredient of Henna. Mutat Res 2003;537:183-99.
24. Thabrew MI, Joice PD, Rajatissa W. A comparative study of the efficacy of Pavetta indica and Osbeckia octandra in the treatment of liver dysfunction. Planta Med 1987;53(3):239-41.
25. Constantin M, Bromont C, Fickat R, Massingham R. Studies on the activity of bepridil as a scavenger of free radicals. Biochem Pharmacol 1990;40(7):1615-22.
26. Bello IA, Ndukwe GI, Audu OT, Habila JD. A bioactive flavonoid from Pavetta crassipes K. Schum. Org Med Chem Lett 2011;1:14.
27. Yogi B, Mishra A. Hepatoprotective effects and antioxidant potential of polyherbal formulation against CCl -induced hepatic injury in albino rats. Curr Tradit Med 2016;2:42-9.
28. Harborne JB. Phytochemical Methods. Vol. 3. London: Chapman & Hall; 1988. p. 117-9.
29. Jain A, Soni M, Deb L, Jain A, Rout SP, Gupta VB, et al. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves. J Ethnopharmacol 2008;115:61-6.
30. Shammugasundaram P. Hepatoprotective and antioxidant activity of Hygrophila auriculata heine Acanthaceac root extract. J Ethanopharmacol 2006;104:124-8.
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How to Cite
Yogi, B., and A. Mishra. “HEPATOPROTECTIVE EFFECTS OF POLYHERBAL FORMULATION AGAINST CARBON TETRACHLORIDE-INDUCED HEPATIC INJURY IN ALBINO RATS: A TOXICITY SCREENING APPROACH”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 1, Jan. 2017, pp. 192-5, doi:10.22159/ajpcr.2017.v10i1.14757.
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