IN-VITRO ANTI-OXIDANT ACTIVITY OF CITRULLUS LANATUS SEED
Objective: The objective of present work is to study the In-vitro anti-oxidant activities of n-Hexane, Chloroform and Ethanol extract of Citrullus lanatus seeds to find out the extract having highest anti-oxidant activity. Â Material and Methods: The In-vitro anti-oxidant activity of the extracts were studied using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, Ferric reducing power activity, Hyderogen peroxide(H2O2) scavenging activity and Nitric Oxide(NO) scavenging activity. The total Phenolic contents and Flavanoid contents were estimated taking Gallic Acid and Quercetin calibration curve respectably. Results: In In-vitro anti-oxidant studies it was found that all the extracts posses In-vitro anti-oxidant activities. But the order of possessing activities were n-hexane>ethanol>chloroform extracts of Citrullus lanatus seeds. The total Phenolic content was highest in n-hexane extract and Total Flavanoid content was highest in ethanol extract. Â Conclusion: It can be concluded that Citrullus lanatusseed extracts possess anti-oxidant activities and the potency of anti-oxidant activities depends on the type of extract. The n-hexane extract of Citrullus lanatus seeds possess highest anti-oxidant activity in-vitro.
Keywords: Citrullus lanatus, In-vitro anti-oxidant, 1,1-diphenyl-2-picryl hydrazyl (DPPH), Total Phenolic Content,Â
Simonian N.A., Coyle J.T. Oxidative stress in neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 1996; 36: 83â€“106.
Dhalla N.S, Temsah R.M., Netticadan T. Role of oxidative stress in cardiovascular diseases. J. Hypertens. 2000; 18: 655â€“673.
Vant Veer, P, Jansen, M.C, Klerk M., Kok F.J. Fruits and vegetables in the prevention of cancer and cardiovascular disease. Public Health Nutr. 2000; 3: 103â€“107.
Bokov A, Chaudhuri A, Richardson A. The role of oxidative damage and stress in aging. Mech. Ageing Dev. 2004,125:811â€“826.
Madamanchi N.R., Vendrov A., Runge M.S. Oxidative stress and vascular disease. Arterioscler. Thromb. Vasc. Biol. 2005; 25:29â€“38.
Bravo L. Polyphenol: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 1998; 56:317â€“333.
Heim K.E., Tagliaferro A.R., Bobilya D.J. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 2002; 13: 572â€“584.
Pier-Giorgio P. Flavonoids as antioxidants. J. Nat. Prod. 2000; 63: 1035â€“1042.
Rice-Evans C., Miller N., Paganga G. Antioxidant properties of phenoilc compounds. Trends Plant Sci. 1997; 2:152â€“159.
Sealbert A., Johnson J., Saltmarsh M. Polyphenols: Antioxidants and beyond. Am. J. Clin. Nutr. 2005; 81: 2155â€“2175.
Ross J.A., Kasum C.M. Dietary flavonoids: Bioavailability, metabolic effects, and safety. Ann. Rev. Nutr. 2002; 22: 19â€“34.
Rice-Evans C.A., Miller N. Maxwell SJ. Prospects for the use of antioxidant therapies. Drugs. 1995; 49: 345.
Rahman A.H.M.M., Anisuzzaman M., Ferdous Ahmed, Rafiul Islam A.K.M. and Naderuzzaman A.T.M.. Study of Nutritive Value and Medicinal Uses of Cultivated Cucurbits, Journal of Applied Sciences Research, 2008; 4(5): 555-558.
Madhavi P, Maruthi Rao, Kamala Vakati, Habibur Rahman, M. Chinna Eswaraiah, Evaluation of Anti-Inflammatory Activity of Citrullus lanatus Seed Oil by In-vivo and In-vitro Models. Int. Res J Pharm. App Sci., 2012; 2(4):104-108.
Alok Bhardwaj, Rajeev Kumar, Vivek Dabas, Niyaz Alam, Evaluation of Anti-Ulcer Activity of Citrullus Lanatus Seed Extract in Wistar Albino Rats. International Journal of Pharmacy and Pharmaceutical Sciences, 2012; 4(5): 135-139.
Okunrobo O. Lucky, Uwaya O. John, Imafidon E. Kate, Osarumwense O. Peter, Omorodion E. Jude, Quantitative determination, Metal analysis and Antiulcer evaluation of Methanol seeds extract of Citrullus lanatus Thunb (Cucurbitaceae) in Rats. Asian Pacific Journal of Tropical Disease. 2012; S1:261-S1265.
Loiy Elsir Ahmed Hassan. In-vitro Antimicrobial activities of chloroformic, hexane and ethanolic extracts of Citrullus lanatus var. citroides. Journal of Medicinal Plants Research. 2011; 5 (8): 1338-1344.
Swapnil Sharma. First report on Laxative activity of citrullus lanatus. Pharmacology online. 2011; 2:790-797.
Naresh Singh Gill. Evaluation of Antioxidant activity of citrullus lanatus Seed extract in Rats., Lat. Am. J. Pharm. 2011; 30(3):429-34.
Madhavi P, Kamala Vakati, and Habibur Rahman. Hepatoprotective Activity of Citrullus Lanatus Seed Oil on CCl4 Induced Liver Damage in Rats. Scholars Academic Journal of Pharmacy, 2012; 1 (1):30-33.
Aruna Poduri, Debra L. Rateri, Shubin K. Saha, Sibu Saha, Alan Daugherty. Citrullus lanatus â€˜sentinelâ€™ (watermelon) extract reduces atherosclerosis in LDL receptor-deficient mice. Journal of Nutritional Biochemistry, 2012; (Article in Press)
Kokate CK, Practical Pharmacognosy, 5th Edn, Vallabh Prakasham, 107-121, 1991.
Hirano R, Sasamoto W, Matsumoto A, Itakura H, Igarashi O, Kondo K. Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. J Nutr Sci Vitaminol (Tokyo). 2001; 47(5):357-362.
Moein S, Moein M, Khoshnoud MJ, Kalanteri T. In vitro antioxidant properties evaluation of 10 Iranian medicinal plants by different methods. Iran Red Crescent Med J. 2012;14(12):771-775.
Ehsan Oskoueian, Norhani Abdullah, Syahida Ahmad, Wan Zuhainis Saad, Abdul Rahman Omar, Yin Wan Ho. Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract. Int J Mol Sci. 2011; 12(9): 5955â€“5970.
Jie Yin, Seong-Il Heo, Myeong-Hyeon Wang. Antioxidant and antidiabetic activities of extracts from Cirsium japonicum roots. Nutr Res Pract. 2008; 2(4): 247â€“251.
Jun Zhao, Tao Liu, Long Ma, Ming Yan, Zhengyi Gu, Yi Huang, Fang Xu, Yu Zhao. Antioxidant and Preventive Effects of Extract from Nymphaea candida Flower on In Vitro Immunological Liver Injury of Rat Primary Hepatocyte Cultures. Evid Based Complement Alternat Med. 2011; 2011: 49:73-76.
Mahfuza khatoon, Ekramul Islam, Rafikul Islam, Aziz Abdur Rahman, AHM Khurshid Alam, Proma Khondkar, Mamunur Rashid, Shahnaj Parvin. Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Res Notes. 2013; 6: 121.
Rahmat Ali Khan, Muhammad Rashid Khan, Sumaira Sahreen, Mushtaq Ahmed. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chem Cent J. 2012; 6: 43.
Sulaiman CT, Indira Balachandran. Total Phenolics and Total Flavonoids in Selected Indian Medicinal Plants. Indian J Pharm Sci. 2012; 74(3): 258â€“260.
Mojab F, Kamalinejad M, Ghaderi N, Vanidipour HR. Phytochemicals screening of some species of Iranian plants. Iran J. Pharm. Res. 2003; 3:77-82.
Catherine A. Rice-Evans, Nicholas J. Miller, George Paganga. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine. 1996; 20(7): 933â€“956.
Wolf Bors and Manfred Saran. Radical Scavenging by Flavonoid Antioxidants. Free Radical Research, 1987; 2(4-6): 289-294.
Catherine A. Rice-Evans, Nicholas J. Miller, George Paganga. Antioxidant properties of phenolic compounds. Trends in Plant Science; 1997; 2(4): 152â€“159.
Kumar, S., D. Kumar and O. Prakash. Evaluation of antioxidant potential, phenolic and flavonoid content of Hibiscus tiliaceus flowers. EJEAF Chem., 2008; 7: 2863-2871.
Sharififar, F., G. Dehghan-Nudeh and M. Mirtajaldini. Major flavonoids with antioxidant activity from Teucrium polium. Food Chem., 2009; 112: 885-888.
Chidambara Murthy KN, Vanitha A, Mahadeva Swamy M, and Ravishankar GA. Antioxidant and Antimicrobial Activity of Cissus quadrangularis L. Journal of Medicinal Food, 2003; 6(2): 99-105.
Park YS, Heo BG, Ham KS, Kang SG, Park YK, Nemirovski A, Tashma Z, Gorinstein S, Leontowicz H, Leontowicz M. Analytical determination of bioactive compounds as an indication of fruit quality. J AOAC Int. 2012 ;95(6):1725-1732.
Oboh G, Akinyemi AJ, Ademiluyi AO. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitro. Exp Toxicol Pathol. 2012;64(1-2):31-36.
Mustafa AG, Singh IN, Wang J, Carrico KM, Hall ED. Mitochondrial protection after traumatic brain injury by scavenging lipid peroxyl radicals. J Neurochem. 2010;114(1):271-280.
Karuppagounder SS, Madathil SK, Pandey M, Haobam R, Rajamma U, Mohanakumar KP. Quercetin up-regulates mitochondrial complex-I activity to protect against programmed cell death in rotenone model of Parkinson's disease in rats. Neuroscience. 2013;16(236):136-148.
Maity S, Chatterjee S, Variyar PS, Sharma A, Adhikari S, Mazumder S. Evaluation of Antioxidant Activity and Characterization of Phenolic Constituents of Phyllanthus amarus Root. J Agric Food Chem. 2013;61(14):3443-3450.
Habbu PV, Mahadevan KM, Kulkarni PV, Daulatsingh C, Veerapur VP, Shastry RA. Adaptogenic and in vitro antioxidant activity of flavanoids and other fractions of Argyreia speciosa (Burm.f) Boj. in acute and chronic stress paradigms in rodents. Indian J Exp Biol. 2010 ;48(1):53-60.
Kumari Madhu, Phytochemical screening and antioxidant activity of in vitro grown plants Clitoria ternatea L. Using dpph assay. Asian J Pharm Clin Res.2013; 6( 2):38-42.
Saswati Roy, M. Dutta Choudhury, S.B. Paul, Antioxidant Potential of Rhizome of Alocasia decipiens Schott. Asian J Pharm Clin Res. 2013; 6( 2):120-122.
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