EVALUATION OF IN-VITRO CYTOTOXIC ACTIVITY OF CHLOROFORM EXTRACT OF SIDA ACUTA BURM.F.
Objective: The aim of the present work was to investigate the in-vitro cytotoxic activity of chloroform extract of Sida acuta Burm.f.
Methods: The in-vitro cytotoxic activity of chloroform extract was performed by MTT assay method against A-431 (Human epidermoid carcinoma) and HeLa (Human cervical cancer) cell lines.
Results: The various concentration of crude chloroform extract (1000, 500, 250, 125, 62.5 Âµg/ml concentration) of Sida acuta whole plant was performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against A-431 cell lines (human epidermoid carcinoma) with an of 375Â±0.00 and HeLa cell lines (human cervix carcinoma with an IC50 (inhibit cell growth by 50%) 610.00Â±2.5. The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the extract indicates that Sida acuta has anticancer activity against A-431-Human epidermoid carcinoma, HeLa-Human cervical cancer cell lines.
Conclusion: The present study concluded that the chloroform extract of Sida acuta Burm.f. posses potent cytotoxic activity.
Keywords: Sida acuta Burm.f., Chloroform extract, Cytotoxic activity, IC50.
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