FREE RADICAL QUENCHING EFFICACY OF VARIOUS EXTRACTS OF C.PICTUS TO COMBAT OXIDATIVE DAMAGE/STRESS: AN IN VITRO STUDY

  • Amala Reddy SRM UNIVERSITY
  • Ganesh Venkatayappa

Abstract

Objectives: The objectives of this research article are to elucidate the antioxidant activity of the sequentially extracted fractions using solvents with a varying range of polarity from the leaves of Costus pictus (Zingiberaceae). The antioxidant efficacy to combat the oxidative stress was evaluated based on their superoxide, hydrogen peroxide, and nitric oxide (NO) scavenging activity of the leaf extract.

Methods: Extraction was performed by sequential extraction methods with low to high polar solvents. Using hexane as a low polar, dichloromethane as medium polar, and ethyl acetate and methanol as high polar, based on their individual boiling points extraction was carried out. The extracts were then evaporated using a rotary evaporator under vacuum and stored in the dry container. Then, antioxidant activity of each extract was evaluated for superoxide, hydrogen peroxide, and NO scavenging activity, ascorbic acid was used as a standard drug for the study of antioxidant activity. The output was statistically interpreted, and the most significant concentration of the best extract with good antioxidant activity was evaluated.

Results: As the methanolic solvent is highly polar and able to retrieve the active lead components from the plant material, was very well correlated with the higher degree of free radical scavenging efficacy. It also exhibited the most and significant inhibition in superoxide radical scavenging, hydrogen peroxide scavenging, and NO scavenging which was about 86±1.23, 94±0.34, and 86±1.87 at 250 µg/ml of extract, respectively. The results from our experiments were in par with the positive control chosen.

Conclusions: The above results help us to substantially conclude that bioactive components are extracted well in high polar solvents such as methanol, which mainly includes flavonoids and related polyphenols. These compounds present in C. pictus may be an active lead for potent antioxidant activities which would need further investigations in molecular level.

Keywords: Costus pictus, Hexane, Dichloromethane, Ethyl acetate, Methanol, Ascorbic acid.

References

1. Anokwuru CP, Anyasor GN, Ajibaye O, Fakoya O, Okebugwu P. Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three Nigerian medicinal plants. Nat Sci 2011;9(7):53.
2. Das K, Tiwari RK, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. J Med Plants Res 2010;8(7):161-7.
3. Prasanna G, Anuradha R. Evaluation of in vitro antioxidant activity of rhizome extract of Drynaria quercifolia L. Int J ChemTech Res
2015 8(11):183-7.
4. Shivshankar MG, Shanmugarajan TS. In vitro potential of plant stress hormone methyl jasmonate for anti arthritis, anti-inflammatory and free radical scavenging activity. Int J Pharm Tech Res 2014;8(7):161-7.
5. Saumya MS, Basha M. In vitro evaluation of free radical scavenging activities of Panax ginseng and Lagerstromea species: A comparative analysis. Int J Pharm Pharm Sci 2011;3(1):165-9.
6. Kamali H, Sani TA, Feyzi P, Mohammad A. Chemical composition and antioxidant activity from Essential oil of Capsella bursa-pastoris. Int J PharmTech Res 2015;8(8):1-4.
7. Jasmine R, Rajasulochana M, Aude R. Evaluation of in vitro antioxidant and anti-inflammatory property exhibited by silver nanoparticles stabilized by Adathoda vasica. J Chem Pharm Res
2016;8(5):128-37.
8. Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and
in vitro methods evaluation of antioxidant activity. Saudi Pharm J
2013;21(2):143-52.
9. Narayanaswamy N, Balakrishnan KP. Evaluation of some medicinal plants for their antioxidant properties. Int J PharmTech Res
2011;3(1):381-5.
10. Narayanaswamy N, Duraisamy A, Balakrishnan KP. Screening of some
2 2 medicinal plants for their antityrosinase and antioxidant activities. Int J
Further investigations are required to find the active component of the
active extract and to confirm the mechanism of action. This article could also be a comprehensive ready reference for those who are interested on antioxidant study.

PharmTech Res 2011;3(2):1107-11.
11. Ottu OJ, Atawodi SE, Onyike E. Antioxidant, hepatoprotective and hypolipidemic effects of methanolic root extract of Cassia singueana in rats following acute and chronic carbon tetrachloride intoxication.
Asian Pac J Trop Med 2013;6:609-15.
12. Pawar SN, Murthy, Pandian SJ. Antioxidant activity of Coldenia procumbens Linn. whole plant methanolic extract. Int J Pharm Pharm Sci 2014;6(1):???.
13. Chanda S, Dave R. In vitro models for antioxidant activity evaluation rhizome and Ziziphus leaves. Int J PharmTech Res 2015;7(4):554-9. rhizome and Ziziphus leaves. Int J PharmTech Res 2015;7(4):554-9.
16. Shiny CT, Saxena A, Gupta SP. Phytochemical and hypoglycaemic activity investigation of Costus pictus plants from Kerala and Tamilnadu. Int J Pharm Sci Invent 2013;2(5):11-8.
17. Singh P, Shrivastava R, Saxena RC, Sharma M, Karchuli MS, Tripathi J.
Statistics
291 Views | 416 Downloads
How to Cite
Reddy, A., and G. Venkatayappa. “FREE RADICAL QUENCHING EFFICACY OF VARIOUS EXTRACTS OF C.PICTUS TO COMBAT OXIDATIVE DAMAGE/STRESS: AN IN VITRO STUDY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 2, Feb. 2017, pp. 178-81, doi:10.22159/ajpcr.2017.v10i2.15011.
Section
Original Article(s)