Screening of active antimicrobial and biological enzymes of microbial isolated from soil in Thailand
Objective: The objectives of this study were to isolate microorganisms and screen for potential antimicrobial activities from the soil.Â
Methods: In this study, a total of 425 isolates were isolated from 100 soil samples. The preliminary screening for antimicrobial activities of theseÂ isolates was performed by modified cross-streak, agar diffusion, and modified icrodilution technique against 16 pathogenic bacteria and fungi.
Results: In the anti-microbial activity, there were three isolates, namely, 277, 303, and 307 exhibited inhibitory activity against methicillin-resistant
Staphylococcus aureus and Salmonella typhimurium respectively. This study also examined the various enzymes producing from soil microorganismsÂ including chitinase, chitosanase, amylase, cellulose, caseinase, gelatinase, esterase, and lipase production of different selective media for 24 and 48 hrs using the direct spot method. The results revealed that 28 isolates could produce various enzymes with strong activity. Most of them producedÂ gelatinase (5.65%) and caseinase (5.18%). There were four isolates that produce broad-spectrum enzyme. In addition, the investigation of selected microorganism identification showed that they can be divided into three groups: Burkholderia spp., Pseudomonas spp., and Rhodococcus spp.
Conclusion: This study demonstrated that the microorganisms from soil are capable of producing potential, antibacterial, and bioactive enzymes.
Keywords: Antimicrobial activity, Extracellular enzyme, Soil microbial, Drug-resistant bacteria.
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