• Santhosh Sigamani Department of Microbiology, Periyar University, Periyar palkalai nagar Salem
  • Sharmila Banu Vm
  • Hemalatha V
  • Venkatakrishnan V
  • Dhandapani R


Objective: The current study focuses on R-Phycoerythrin pigment production from Seaweed using different chemical and physical conditions. 

Methods: In the present study Seaweed was collected from Rameshwaram and identified by CS-MCRI Institute, Mandapam. The collected seaweed was then washed using distilled water for further processing. Using a sterile knife the seaweed was cut into small pieces. The chopped seaweeds were then weighed and subjected to different optimization procedures for pigment production. These equally weighed seaweeds were treated with three varying Buffers at different pH, the buffer showing better O.D value was subjected to different Cell disruption techniques and finally freeze thawed at different temperature stress.

Results: The seaweeds were subjected to different chemical and physical stress conditions for R-phycoerythrin production. On optimizing the different buffer solutions for pigment production Sodium phosphate buffer showed maximum O.D of 0.215 when compared to other buffers whereas on providing different pH conditions the O.D value obtained was high at pH 7.2. Different cell disruption techniques were followed for pigment production using the sodium phosphate buffer at pH 7.2 and freeze thaw method was found suitable for the highest pigment production with O.D value of 0.441. Hence after optimization of different extraction procedures, cell disruption followed by freeze & thaw method (−20°C and 25°C) showed maximum R-phycoerythrin content. 

Conclusion: From the findings, it was also observed that the primary metabolites produced by these organisms may serve as potential bioactive compounds of interest in the Food industries as natural colourant and in cosmetic industries.

Keywords: Seaweeds, Extraction, Phycoerythrin, Optimization, Cell disruption, Sonication.


1. De Barros-Barreto MB, Marinho LC, Reis RP, Da Mata CS, Ferreira PC. Kappaphycus alvarezii (Gigartinales, Rhodophyta) cultivated in Brazil: Is it only one species? J Appl Phycol 2013;25(4):1143-9.
2. Ask E, Batibasaga A, Zertuche-Gonzalez J, De San M. Three Decades of Kappaphycus alvarezii (Rhodophyta) Introduction to Non-endemic Locations. Proceedings of International Seaweed Symposium; 2003. p. 49-57.
3. Machado S, Brijesh K, Troy D. Ostreasterol. Seaweed Sustainability: Food and Non-Food Applications. Vol. 4. London, UK: Elsevier; 2004. p. 402-12.
4. Mc Hugh DJ. A Guide to Seaweed Industry. FAO Fisheries and Aquaculture Technical Paper. T441; 2003. p. 118.
5. Kumar SK, Ganesan K, Rao SP. Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty – An edible seaweed. Food Chem 2008;107(1):289-95.
6. Rajasulochana P, Dhamotharan R, Krishnamoorthy P. Primary phytochemical analysis of Kappaphycus sp. J Am Sci 2009;5(2):91-6.
7. Fayaz M, Namitha KK, Murthy KN, Swamy MM, Sarada R, Khanam S, et al. Chemical composition, iron bioavailability, and antioxidant activity of Kappaphycus alvarezzi (Doty). J Agric Food Chem 2005;53(3):792-7.
8. Omer TA. Isolation and identification of some chemical constituents in two different types of fresh water macro algae in Bestansur village in Suleiman city Kurdistan region (North Iraq) by HPLC techniques. IOSR J Appl Chem 2013;4(3):45-55.
9. Dunford NT, King JW. Phytosterol enrichment of rice bran oil by a supercritical carbon dioxide fractionation technique. J Food Sci
10. Pugalendren S, Sarangam B, Rengasamy R. Extraction of R-phycoerythrin from Kappaphycus alvarezii (Doty) Doty ex Silva and analyses of its physico-chemical properties. J Acad Ind Res 2012;1(7):2278-5213.
11. Sekar S, Chandramohan M. Phycobiliproteins as a commodity: Trends in applied research, patents & commercialization. J Appl Phycol 2008;20(2):113-36.
12. Moore RE. Algal non-isoprenoids. In: Scheuer PJ, editor. Marine Natural Products, Chemical and Biological Perspective. New York: Academic Press; 19781. p. 44-171.
13. Konig GM, Wright AD, Sticher O, Anghofer CK and Pezutto JM.
Biological activities of selected marine natural products. Planta Med.
14. Hemlata, Pandey G, Bano F, Fatma T. Studies on Anabaena sp. NCCU-9 with special reference to phycocyanin. J Algal Biomass Utln 2011;2(1):30-51.
15. Unnikrishnan PS, Suthindhiran K, Jayasri MA. Antidiabetic potential of marine algae by inhibiting key metabolic enzymes. Front Life Sci 2015;8(2):149-59.
16. Indriatmoko, Heriyanto, Limantaraa L, Hardo T, Brotosudarmoa P. Composition of photosynthetic pigments in a red algae
Kappaphycus alvarezii cultivated in different depths. Proc Chem
17. Yokoya NS, Necchi O, Martins AP, Gonzalez SF, Plastino EM. Growth responses and photosynthetic characteristics of wild and phycoerythrin- deficient strains of Hypnea musciformis (Rhodophyta). J Appl Phycol 2007;19:197-205.
18. Chia YY, Kanthimathi MS, Rajarajeswaran J, Khoo KS, Cheng HM.
Antioxidant, antiproliferative, genotoxic and cytoprotective effects
of the methanolic extract of Padina tetrastromatica on human breast adenocarcinoma and embryonic fibroblast cell lines. Front Life Sci
19. Velkanni K, Rengaswamy R. Effect of nitrate on growth, pigments and k-carrageenan contents of Hypnea valentiae (Turn.) Mont. Mahasagar 1992;52(4):150-62.
20. Saenger P. The water soluble pigments of the red algae, Lenormandia prolifera. Phycologia 1969;7(1):59-64.
21. Dumay J, Morançais M, Munier M, Le Guillard C, Fleurence J.
Phycoerythrins: Valuable proteinic pigments in red seaweeds. Advances in Botanical Research. Vol. 71. Ch. 11. Burlington: Academic Press; 2014. p. 321-43.
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How to Cite
Sigamani, S., S. B. Vm, H. V, V. V, and D. R. “OPTIMIZATION STUDY ON EXTRACTION & PURIFICATION OF PHYCOERYTHRIN FROM RED ALGAE KAPPAPHYCUS ALVAREZII”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 2, Feb. 2017, pp. 297-02, doi:10.22159/ajpcr.2017.v10i2.15598.
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