EFFECTS OF ORAL ALFACALCIDOL ON MATURATION OF DENDRITIC CELLS IN GRAVES’ DISEASE PATIENTS: A DOUBLE-BLINDED RANDOMIZED CLINICAL TRIAL


Dyah Purnamasari, Samsuridjal Djauzi, Siti Setiati, Alida Harahap, Tjokorda Gde Pemayun, Joedo Prihartono, Pradana Soewondo

Abstract


Maturity level of dendritic cells (DC) plays a pivotal role in initiating and regulating autoimmunity. In graves’ disease (GD), DCs have more active immune responses than those in healthy subjects. Our previous study demonstrated immunoregulatory effects of in vitro 1,25-D3 on maturation of DC in GD patients. This study aims to evaluate the effect of oral 1α-D3 on DC maturation in GD patients.

 

Methods: Twenty five GD patients with thyrotoxicosis were divided into two groups: 12 GD patients receiving oral 1α-D3 and 13 GD patients receiving placebo, in addition to treatment of propylthiouracil. Comparison of DC maturation were performed before and after the oral 1α-D3. DC maturation was assessed based on the expression of DC markers (HLA-DR, CD80, CD40, CD83, CD14 and CD206) and the ratio of cytokines interleukin-12/IL-10.

Results: After 8 weeks, 8 out of 12 GD patients in treatment group and 6 out of 13 GD patients in placebo group still had high fT4 level. The expression of CD80 decreased (p=0.48) and CD206 increased (p=0.47) insignificantly among treatment group. The IL-12/IL-10 ratio decreased along with the improvement of fT4 level in both groups. No difference of the IL-12/IL10 ratio between treatment and placebo group.

Conclusion: The effects of oral 1α-D3 on DC maturation of GD patients have not been clearly demonstrated in this study yet.

 

 


Keywords


vitamin D, graves’ disease, dendritic cells

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About this article

Title

EFFECTS OF ORAL ALFACALCIDOL ON MATURATION OF DENDRITIC CELLS IN GRAVES’ DISEASE PATIENTS: A DOUBLE-BLINDED RANDOMIZED CLINICAL TRIAL

Keywords

vitamin D, graves’ disease, dendritic cells

DOI

10.22159/ajpcr.2017.v10i6.16110

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 100-104

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

76 Views | 64 Downloads

Authors & Affiliations

Dyah Purnamasari
Division of Endocrinology, Department of Internal Medicine, Faculty of Medicine University of Indonesia.
Indonesia

Samsuridjal Djauzi
Division of Allergy Immunology, Department of Medicine, Faculty of Medicine Universitas Indonesia, Indonesia
Indonesia

Siti Setiati
Division of Geriatrics, Department of Medicine, Faculty of Medicine Universitas Indonesia, Indonesia
Indonesia

Alida Harahap
Division of Immunology, Departement of Clinical Pathology, Faculty of Medicine Universitas Indonesia, Indonesia
Indonesia

Tjokorda Gde Pemayun
Division of Endocrinology, Department of Medicine, Faculty of Medicine Universitas Diponegoro, Indonesia
Indonesia

Joedo Prihartono
Departement of Community Medicine, Faculty of Medicine Universitas Indonesia, Indonesia
Indonesia

Pradana Soewondo
Division of Endocrinology, Department of Medicine, Faculty of Medicine Universitas Indonesia, Indonesia
Indonesia


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