GAS CHROMATOGRAPHY-MASS SPECTROSCOPY STUDIES ON CESTRUM NOCTURNUM MACERATED METHANOLIC EXTRACT
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i3.16134Abstract
ABSTRACT
Objective: Phytochemical screening has revealed the presence of alkaloids, glycosides, carbohydrates, steroids, flavonoids, saponins, and tannins from
methanolic extract of Cestrum nocturnum. In this research, we have identified and confirmed the structures of the constituents by gas chromatography–
mass spectrometry (GC-MS) from the methanolic extract of C. nocturnum leaves.
Methods: Shade-dried leaves were powdered and extracted with methanol by cold extraction procedures and the crude extract obtained was used
for GC-MS.
Results: There were total five components prominently eluted from C. nocturnum extract, viz., ethyl citrate, phytol, 4-isobutylmorpholine,
n-hexadecanoic acid, and α-amyrin.
Conclusion: GC-MS studies helped to recognize the phytochemical constituents based on their retention time and verification of MS libraries. In
future, C. nocturnum leaves may be subjected to different extraction procedures and number of elucidating phytoconstituents can be studied for their
ethnopharmacological relevance citing the literature.
Keywords: Cestrum nocturnum, Gas chromatography-mass spectroscopy, α-Amyrin, n-Hexadecanoic acid, Ethyl citrate, Phytol, 4-Isobutylmorpholine.
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REFERENCES
Harborne JB. In: Phytochemical Methods. 3rd ed. New Delhi: Springer Publication House; 2005. p. 1-295.
Buckingham J. In: Dictionary of Natural Products. 1st ed. London: Chapman and Hall; 1994. p. 640-6.
Samuelsson G. In: Drugs of Natural Origin. 5th ed. Sweden: Swedish Pharmaceutical Press; 2004. p. 154-5.
Doshi GM, Nalawade VV, Mukadam AS, Chaskar PK, Zine SP, Somani RR et al. Structural elucidation of chemical constituents from Benincasa hispida seeds and Carissa congesta roots by Gas chromatography - mass spectroscopy. Pharmacognosy Res 2015;7(3):282-93.
Vazquez LH, Palazon J, Ocana AN. In: Phytochemicals. Vienna: InTech Publishers; 2012. p. 487-502.
Hegnauer R. In: Waterman PG, Grundon MF, editors. Chemistry and Chemical Taxonomy of the Rutales. London: Academic Press; 1983. p. 401-40.
Hill RA. In: The Chemistry of Natural Products. London: Blackie Academic & Professional; 1993. p. 106-39.
Anonymous. In: The Wealth of India. New Delhi, India: Council of Scientific & Industrial Research; 1950. p. 125-8.
Warrier PK, Nambiar VPK, Ramankutty C. In: Indian Medicinal Plants. New Delhi, India: Orient Longman Private Limited; 1976. p. 224-34.
Kokate CK. In: Practical Pharmacognosy. New Delhi, India: Vallabh Prakashan; 1989. p. 111-25.
Khandelwal KR. In: Practical Pharmacognosy. 10th ed. New Delhi, India: Nirali Prakashan; 2003. p. 149-56.
Doshi GM, Mukadam AS. Pharmacognostic quantification of flavonoids by high performance thin layer chromatography and in vitro cell line study on developed herbal formulation from Cestrum nocturnum plant extract. Int J Green Pharm 2016;10(3):183-92.
Park HM, Misra M, Drzal LT, Mohanty AK. Green nanocomposites from cellulose acetate bioplastic and clay: Effect of eco-friendly triethyl citrate plasticizer. Biomacromolecules 2004;5(6):2281-8.
Selvarani K, Stella Bai GV. GC-MS analysis of biologically active compounds in Cayratia pedata (Lam) leaf and callus extracts. Int J Chem Stud 2014;2(3):51-6.
Sahoo S, Jena S, Sahoo A, Ray A, Nasim N, Kar N, et al. GC-MS analysis and evaluation of bioactivities of Kaempferia parishii - A natural source of totarol. Int J Pharm Pharm Sci 2016;8(5):182-6.
Venkatachalapathi A, Paulsamy S, Thambiraj J. Antimicrobial efficacy of the ethnomedicinal plant species, Canarium strictum Roxb. Int J Pharm Pharm Sci 2016;8(2):339-41.
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