A COMPARATIVE STUDY OF IN-VITRO NITRIC OXIDE SCAVENGING ACTIVITY OF BALOFLOXACINVS PRULIFLOXACIN
Objectives: To compare the free radical scavenging activity and antioxidant potentials of balofloxacin versus prulifloxacin.
Methods: Nitric oxide (NO) radical scavenging activity was determined using the method of Garret (1964). Sodium nitroprusside in aqueous solution at physiological pH spontaneously generates NO, which interacts with oxygen to produce nitrite ions which can be determined by the use of Griess ilosvay reaction. 2 mm of 10 mm sodium nitroprusside in 0.5 ml phosphate buffer saline (pH 7.4) was mixed with 0.5 ml prulifloxacin and balofloxacin at various concentrations and the mixture incubated at 25Â°C for 150 minutes. From the incubated mixture 0.5 ml was taken out and added into 1.0 ml sulfanilic acid reagent (30 in 20% glacial acetic acid) and incubated at room temperature for 5 minutes. Finally, 1.0 ml naphthylethylenediamine dihydrochloride (0.1% w/v) was mixed and incubated at room temperature for 30 minutes. The resultant absorbance was recorded at 540 nm usinga spectrometer. The percentage inhibition was calculated using the formula: Percentage inhibition = Â Abscontrol Â âˆ’ AbssampleÂ Â Ã—Â 100
Results: At concentration of 10, 20, 30, and 40 Âµg/ml percentage inhibition observed with prulifloxacin and balofloxacin was 40.94, 53.09, 66.25,88.06 and 34.49, 49.09, 60.42, and 73.14, respectively.
Conclusion: Thus, this in vitro study suggests that both prulifloxacin and balofloxacin possess antioxidant properties. At the lower doses both the drugs show similar antioxidant profile, while at the higher doses the antioxidant potential of prulifloxacin is significantly high than balofloxacin. However, the preference of selecting one over the other depends on the indication as well as the risk-benefit ratio.
Keywords: In vitro, Nitric oxide, Prulifloxacin, Balofloxacin.
2. Kim GH, Kim JE, Rhie SJ, Yoon S. The role of oxidative stress in neurodegenerative diseases. Exp Neurobiol 2015;24(4):325-40.
3. Oliphant CM, Green GM. Quinolones: A comprehensive review. Am Fam Physician 2002;65(3):455-65.
4. Zahin M, Aqil F, Ahmad I. The in vitro antioxidant activity and total phenolic content of four Indian medicinal plants. Int J Pharm Pharm Sci 2009;1 Suppl 1:88-95.
5. Subhashini N, Nagarajan G, Kavimani S. In vitro antioxidant and anticholinesterase activities of Garcinia combogia. Int J Pharm Pharm Sci 2011;3:129-32.
6. Saux NL. The treatment of acute bacterial sinusitis: No change is good medicine. CMAJ 2008;178(7):865-6.
7. Karthik VP, David DC. In-vitro nitric oxide scavenging activity of ethanol extract of Canthium coramandelium. Int J Pharm Bio Sci 2015;6(4):695-700.
8. Thiruvengadam R, Sankar S. Comparison of in vitro antioxidant activity of olmesartan and amlodipine. Int J Pharmacol Clin Sci 2015;4(4):90-3.
9. Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 2009;7(1):65-74.
10. KÅ‚adna A, Michalska T, BerczyÅ„ski P, Kruk I, Aboul-Enein HY. Evaluation of the antioxidant activity of tetracycline antibiotics in vitro. Luminescence 2012;27(4):249-55.
11. Parul R, Kundu SK, Saha P. In vitro nitric oxide scavenging activity of methanol extracts of three Bangladeshi medicinal plants. Pharm Innov J 2013;1(12):83.
12. Talla V, Veerareddy PR. Oxidative stress induced by fluoroquinolones on treatment for complicated urinary tract infections in Indian patients. J Young Pharm 2011;3(4):304-9.
13. Goswami M, Mangoli SH, Jawali N. Involvement of reactive oxygen species in the action of ciprofloxacin against Escherichia coli. Antimicrob Agents Chemother 2006;50(3):949-54.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.