APOPTOSIS INDUCTION EFFECT OF CURCUMIN AND ITS ANALOGS PENTAGAMAVUNON-0 AND PENTAGAMAVUNON-1 ON CANCER CELL LINES

Muhammad Da'i; Andi Suhendi; Edi Meiyanto; UMAR  A JENIE; Masashi Kawaichi

doi:10.22159/ajpcr.2017.v10i3.16311

Authors

Muhammad Da'i Faculty of Pharmacy Universitas Muhammadiyah Surakarta
Andi Suhendi Faculty of Pharmacy Universitas Muhammadiyah Surakarta
Edi Meiyanto Universitas Gadjah Mada
UMAR A JENIE Faculty of pharmacyUniversitas Gadjah Mada
Masashi Kawaichi Nara Institute of Science and Technology, Ikoma, Japan

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i3.16311

Abstract

ABSTRACT
Objectives: This experiment aims to investigate the apoptosis effect of curcumin and its analogs pentagamavunon-0 (PGV-0) and PGV-1 on normal
and other cancer cell lines.
Methods: Growth inhibition effect was investigated using the MTT method. Double staining used acridine orange, 2-(4-aminodiphenyl)-6-
indolcarbamidine dihydrochloride and ethidium bromide was performed to determine morphological changes of cells. Detection of PARP, caspase-3,
PUMA and BAX using a western blot method was conducted to elucidate the apoptosis effect of the compounds.
Results: PGV-1 (2.5 Î¼M) and PGV-0 (5.0 Î¼M) could inhibit T47D-cell growth on 72 h observation, but not for curcumin. DNA staining showed PGV-1
has the strongest apoptosis induction effect on T47D-cells compared to PGV-0 and curcumin as well. Western blot analysis resulted in cleavage PARP
(83 kD) on HeLa, T47D, and MCF-7 cells treated with PGV-1 (2.5 Î¼M), PGV-0 (5.0 Î¼M). Curcumin (10.0 Î¼M) just induced apoptosis on T47D-cell and
MCF-7 cell, but not HeLa cell. Cleavage PARP resulted by apoptosis process in the cell. PGV-1 (2.5 Î¼M) had a stronger apoptosis effect compared to
PGV-0 (5.0 Î¼M) and curcumin (10.0 Î¼M) based on cleaved PARP result qualitatively. On the normal cell (NH3T3), cells that were treated with the
compounds resulted in a negative cleavage PARP. This result indicated that the compounds were part of a selectively induced cancer cell line apoptosis
process.
Conclusion: Curcumin, PGV-0 and PGV-1 could inhibit cell growth by induce apoptosis on cancer cells but not on normal cells, which PGV-1 has
strongest apoptosis induction effect on cancer cell lines.
Keywords: Curcumin and analogs, Apoptosis, Cancer cell lines.

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Author Biography

Andi Suhendi, Faculty of Pharmacy Universitas Muhammadiyah Surakarta

Chemical Pharmacy Department

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Fig. 3: Pentagamavunon-1 (2.5 Î¼g/mL). (a) And PGV-0 (5.0 Î¼g/mL), (b) induced apoptosis on HeLa, T47D and MCF-7. On higher concentration, both compounds could not induce apoptosis on NIH3T3 (normal cell) curcumin, (c) induced apoptosis on T47D and MCF-7 but not on HeLa and NIH3T3, (d) Further investigation of PGV-1 could activate caspase-3 on T47D-cells but not on HeLa and NIH3T3, increased PUMA and BAX on MCF-7 cell but not on HeLa and NIH3T3 cell

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