IN VITRO EVALUATION OF ANTI MYCOTIC ACTIVITY OF ETHANOLIC EXTRACT OF GLYCYRRHIZA GLABRA
Keywords:Glycyrrhiza glabra, disc diffusion, zone of inhibition, anti mycotic activity
Objective: The aim of the present study was to assess the anti fungal activity of ethanolic extract of Glycyrrhiza glabra. Glycyrrhiza glabra commonly called as liquorice or sweet root is one of the oldest known herbal remedies famous for its vast variety of therapeutic properties. Ethanolic extract of Glycyrrhiza glabra was tested for antifungal activity against Candida albicans, Aspergillus niger, Aspergillus fumigates, Mucor spp and Penicilium marneffei.
Method: Disc diffusion technique was followed for screening anti fungal activity. The discs were loaded with 100Âµl of ethanolic extracts at different concentrations [500ug/disc,1000ug/disc and 2000Âµg/disc]. Positive controls used were fluconazole (10 mcg/disc) and amphotericin B (100 units/disc).
Result : After incubation at 28oC for 48 hours, the zone of Inhibition was measured.
Conclusion: The extract at different concentrations showed varying degree of antifungal activity against the micro organisms tested compared to standard.
KEYWORDS: Glycyrrhiza glabra, disc diffusion, zone of inhibition, anti mycotic activity.
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