IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)


Shirly Kumala, Ng Vini Aprilia, Partomuan Simanjuntak

Abstract


Objective: Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini  L).  The secondary metabolites of this plant has the potent antibacterial efficacy  as well as diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.

Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli were fermented in Potato Dextrose Yeast (PDY) to produce  large scale of the metabolites.  Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.

Results:  Antibacterial  test  results  indicated that fraction III had  the antibacterial  activity Staphylococcus aureus with  inhibition zone diameter  of 10.7 mm but no observed  antibacterial activity against Escherichia coli.  Furthermore, identification  by GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.

Conclusion:  In conclusion, secondary metabolites isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity.

 Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity


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About this article

Title

IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)

DOI

10.22159/ajpcr.2017.v10i4.16725

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 283-286

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

134 Views | 105 Downloads

Authors & Affiliations

Shirly Kumala
Faculty of Pharmacy Pancasila University Jakarta Indonesia
Indonesia

Ng Vini Aprilia

Partomuan Simanjuntak


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