IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM JAMBLANG PLANT ( Eugenia cumini L)
Objective: Colletotrichium capsiciÂ endophytic fungi isolated from Jamblang plant (Eugenia cumini Â L). Â The secondary metabolites of this plant has the potent antibacterial efficacy Â as well asÂ diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.
Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteriaÂ Staphylococcus aureus, Escherichia coli wereÂ fermented inÂ Potato Dextrose YeastÂ (PDY) to produce Â large scale of the metabolites. Â Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3,Â n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.
Results: Â Antibacterial Â test Â results Â indicatedÂ that fraction IIIÂ had Â the antibacterial Â activityÂ StaphylococcusÂ aureusÂ with Â inhibition zoneÂ diameter Â of 10.7Â mm but no observed Â antibacterial activityÂ againstÂ EscherichiaÂ coli. Â Furthermore, identificationÂ byÂ GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.
Conclusion: Â In conclusion, secondary metabolites isolated from Jamblang plantsÂ branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity.
Â Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity
2. Oliveira GF, Furtado NA, Filho SA, Martins CH, Bastos JK, Cunha WR, et al. Antimicrobial activity of Syzygium cumni (Myrtaceae) leaves extract. Braz J Microbiol 2007;38(2):381-4.
3. Yadav M, Yadav A, Kumar S, Sharma D, Yadav JP. Evaluation of in vitro antimicrobial potential of endophytic fungi isolated from Eugenia jambolana Lam. Int J Pharm Pharm Sci 2014;6(5):208-11.
4. Dalimarta S. Atlas Tumbuhan Obat Indonesia. Jilid 3. Jakarta: Balai Pustaka. Puspa Swara; 2003. p. 19-23.
5. Petrini O, Sieber TN, Toti L, Viret O. Ecology, metabolite production, and substrate utilization in endophytic fungi. Nat Toxins 1992;1(3):185-96.
6. Kalyanasundaram I, Nagamuthu J, Muthukumaraswamy S. Antimicrobial activity of endophytic fungi isolated and identified from salt marsh plant in valiar estuary. J Microbiol Antimicrob Acad J 2015;7(2):13-20.
7. Tayung K, Barik BP, Jha DK, Deka DC. Identification and characterzation of antimicrobial metabolite from an endophytic fungus Fusarium solani isolated from bark of Himalaya yew. Mycosphere 2011;2(3):203-13.
8. Stanbury PF, Whitaker A. Principles of Fermentation Technology. Oxford: Perganon Press; 1984. p. 1-9, 79-80, 169-92.
9. Kumala S, Agustina E, Wahyudi P. Antimicrobial activity test of secondary metabolites of endophytic fungus Trengguli (Cassia futula L). J Nat Prod Indones 2007;6(2):46-8.
10. Kumala S, Izzati H. Isolation IPG3-1 and IPG3-3 endophytic fungi from Delima (Punica granatum LINN.) Twigs and in vitro assesment of their antimicrobial activity. Int Res J Pharm 2013;4(6):49-53.
11. Grant DW. Capilarry Gas Chromatography. Chichester: John Wiley and Sons Limited; 1996. p. 1-14.
12. Purwantini I, Wahyono W, Mustofa M, Asmah R. Isolation of endophytic fungi from Artemisia annua L and identification of their antimicrobial compound using bioautography method. Int J Pharm Pharm Sci 2015;7(12):95-9.
13. Lestari Y, Velina Y, Rahminiwati M. Metabolites activity of endophytic Streptomyces sp, IPBCC, B.15.1539 from Tinospora cripsa L, MIERS; Î±-glucosidase inhibitor and anti-hyperglycemic in mice. Int J Pharm Pharm Sci 2015;7(6):235-9.
14. Prescott LM, Harley JP, Klein DA. Microbiology. 3rd ed. Dubuque, IA: Wm.C Brown Publishers; 1996. p. 37-58.
15. Bauman RW. Microbiology with Diseases by Taxonomy. San Francisco: Pearson Benjamin Cummings; 2007. p. 55-74.
16. Gowrish A, Vagdevi HM, Rajashekar H. Phytochemical screening and antomicrobial activity of Leucas marrubioides DESF. Root extracts. Int J Pharm Pharm Sci 2016;8(12):209-12.
17. Maryati, Fauzia RS, Rahayu T. Antibacterial activity test of Ocimum basillicum L, toward Staphylococcus aureus and Escherichia coli. J Penelitian Sains Technol 2007;8(1):30-8.
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