• Fazeela Mahaboob Begum SM
  • Hemalatha S School of Life Sciences, B.S.Abdur Rahman University, Chennai


Objective: The current study was intended to investigate and characterize the phytoconstituents of  Sargassum wightii ,evaluate its anti diabetic  and anti inflammatory potential under in silico and in vitro conditions.

Methods: The marine algae S.wightii was extracted with ethanol. The ethanolic extract was screened for various phytoconstituents, quantified for total flavonoid and polyphenol content. FTIR and Mass spectrometry was used for characterizing the ethanolic extract. Antidiabetic potential of the phytoconstituents was analysed by molecular docking and enzyme inhibition assays. The anti inflammatory potential was evaluated by albumin denaturation assay.

Results: The phytochemical screening revealed the presence of flavonoids, polyphenols, alkaloids, tannins, carbohydrates, proteins, oils and fat present in the ethanol extract. The FTIR analysis showed the presence of α,b unsaturated ketone, alcohol and ether groups in the extract.  MS analysis identified l - (+) - Ascorbic acid 2, 6 dihexadeconoate and Dotriacontyl isopropyl ether in the ethanolic extract. The molecular docking studies revealed that l - (+) - Ascorbic acid 2, 6 dihexadeconoate interacted with both α amylase and α glucosidase with a consensus score of 5.The results of  in vitro analysis showed that the ethanolic extract exhibited strong inhibitory activity on α amylase (IC 50 8mg/ml) and α glucosidase (IC 50 6mg/ml) respectively. The ethanolic extract also inhibited the formation of advanced glycation end products (IC 50 10mg/ml).Further the ethanolic extract inhibited the denaturation of albumin (IC 50 2.5 mg/ml) there by revealing the anti inflammatory potential of S.wightii.

Conclusion: Hence it can be concluded that S.wightii possess anti diabetic and anti inflammatory potential which may be due to the presence of l - (+) - Ascorbic acid 2, 6 dihexadeconoate and flavonoids, polyphenols, alkaloids, tannins, carbohydrates, proteins, oils and fat present in the extract.

Key words: Sargassum wightii, α amylase, α glucosidase, advanced glycation end products, molecular docking.


1. World Health Organization. Global Report on Diabetes. Geneva: World Health Organization; 2016.
2. Diabetes Globally - Diabetes Australia. Available from:
3. Ulrich P, Cerami A. Protein glycation, diabetes, and aging. Recent Prog Horm Res 2001;56:1-21.
4. Singh R, Barden A, Mori T, Beilin L. Advanced glycation end-products: A review. Diabetologia 2001;44(2):129-46.
5. Aronson D. Cross-linking of glycated collagen in the pathogenesis of arterial and myocardial stiffening of aging and diabetes. J Hypertens 2003;21(1):3-12.
6. Kwon Y, Apostolidis E, Shetty K. Evaluation of pepper (Capsicum annuum) for management of diabetes and hypertension. J Food Biochem 2007;31(3):370-85.
7. Matsui T, Ogunwande IA, Abesundara KJ, Matsumoto K. Anti-hyperglycemic potential of natural products. Mini Rev Med Chem 2006;6(3):349-56.
8. Pravinkumar VI, Gokul ST. Adverse effects of metformin in combination with glimepiride and glibenclamide in patients with Type 2 diabetes mellitus. Asian J Pharm Clin Res 2012;5(1):108-10.
9. Lakshmi SM, Rani KS, Reddy T. A review on diabetes milletus and the herbal plants used for its treatment. Asian J Pharm Clin Res 2012;5(4):15-21.
10. Chandrasekaran M, Venkatesalu V, Raj G, Krishnamoorthy S. Antibacterial properties of various extracts of Sargassum wightii against multidrug resistant bacterial strains. Phykos 2014;44(2):17-28.
11. Kumar K, Anbu J, Sumithra MA, Sathish R. Influence of ethanolic leaf extract of Sargassum wightii and Adiantum capillus on histamine induced asthma in animal model. Int J Pharm Pharm Sci 2012;4(4):121-3.
12. Syad AN, Shunmugiah KP, Kasi PD. Antioxidant and anti-cholinesterase activity of Sargassum wightii. Pharm Biol 2013;51(11):1401-10.
13. Mohapatra L, Bhattamishra S, Panigrahy R, Parida S, Pati P. Antidiabetic effect of Sargassum wightii and Ulva fasciata in high fat diet and multi low dose streptozotocin induced Type 2 diabetic mice. UKJPB 2016;4(2):13-23.
14. Evans WC, Saunders WB. Trease and Evan’s Pharmacognosy. Tokyo: Elsevier; 2001. p. 1-579.
15. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
16. Zhishen I, Mengcheng T, Jianming W. The determination of flavanoids contents in mulberry and their scavenging effects on super radicals. Food Chem 1999;64:555-9.
17. Gurav N, Kardani K, Solanki B, Patel B. Quantification of phenolic compound gallic acid in polyherbal ranger capsule by high performance chromatographic method. IJPSR 2014;25(31):183-7.
18. McCue PP, Shetty K. Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asia Pac J Clin Nutr 2004;13(1):101-6.
19. Andrade-Cetto A, Becerra-Jiménez J, Cárdenas-Vázquez R. Alfa-glucosidase-inhibiting activity of some Mexican plants used in the treatment of Type 2 diabetes. J Ethnopharmacol 2008;116(1):27-32.
20. Matsuura N, Aradate T, Sasaki C, Kojima H, Ohara M, Hasegawa J. Screening system for the Maillard reaction inhibitor from natural product extracts. J Health Sci 2002;48(6):520-6.
21. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci 2010;2(1):146-55.
22. Bailey CJ, Day C. Traditional plant medicines as treatments for diabetes. Diabetes Care 1989;12(8):553-64.
23. Johnson M, Petchiammal E, Janakiraman N, Babu A, Malar TR, Sivaraman A. Phytochemical characterization of brown seaweed Sargassum wightii. Asian Pac J Trop Dis 2012;2 Suppl 1:109-13.
24. Jimenez-Escrig A, Jimenez-Jimenez I, Pulido R, Saura-Calixto F. Antioxidant activity of fresh and processed edible seaweeds. J Sci Food Agric 2001;81(5):530-4.
25. Ganesan P, Kumar CS, Bhaskar N. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresour Technol 2008;99(8):2717-23.
26. Meenakshi S, Gnanambigai DM, Mozhi ST, Arumugam M, Balasubramanian T. Total flavanoid and in vitro antioxidant activity of two seaweeds of Rameshwaram Coast. GJP 2009;3(2):59-62.
27. Akinmoladun AC, Ibukun EO, Afor E, Akinsinlola BL, Onibon TR, Akinboboye AO, et al. Chemical constituents and antioxidant activity of Alstonia boonei. Afr J Biotechnol 2007;6(10):1197.
28. Kumar A, Shrabani P, Pingalkumari S, Thirugnanasambandan S, Kathiresan K. Antibacterial and phytochemical assessment on various extracts of Ipomoea pes-caprae (L.) R. Br through FTIR and GC-MS spectroscopic analysis. Asian J Pharm Clin Res 2014;7(3):134-8.
29. Botzki A, Rigden DJ, Braun S, Nukui M, Salmen S, Hoechstetter J, et al. L-ascorbic acid 6-hexadecanoate, a potent hyaluronidase inhibitor. X-ray structure and molecular modeling of enzyme-inhibitor complexes. J Biol Chem 2004;279(44):45990-7.
30. Unnikrishnan PS, Suthindhiran K, Jayasri MA. Antidiabetic potential of marine algae by inhibiting key metabolic enzymes. Front Life Sci 2015;8(2):148-59.
31. Senthil L, Kumar TV, Geetharamani D, Maruthupandi T. Screening of seaweeds collected from Southeast coastal area of India for α-amylase inhibitory activity, antioxidant activity and biocompatibility. Int J Pharm Pharm Sci 2013;5(1):240-4.
32. Murugesan N, Vember S, Damodharan C. Studies on erythrocyte membrane IV: In vitro hemolytic activity of oleander extract. Toxicol Lett 1981;8(1-2):33-8.
33. Piero NM, Joan NM, Cromwell KM, Joseph NJ, Eliud NN, Wilson NM, et al. Herbal management of diabetes mellitus: A rapidly expanding research avenue. Int J Curr Pharm Res 2012;4(2):1-4.
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How to Cite
Begum SM, F. M., and H. S. “CHARACTERISATION, IN SILICO AND IN VITRO DETERMINATION OF ANTIDIABETIC AND ANTI INFLAMMATORY POTENTIAL OF ETHANOLIC EXTRACT OF SARGASSUM WIGHTII.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 4, Apr. 2017, pp. 297-01, doi:10.22159/ajpcr.2017.v10i4.16742.
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