IN VITRO - ANTIOXIDANT AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF AEGLE MARMELOS (L.) CORREA. LEAF EXTRACT

  • Samidha M Pawaskar Department of Biochemistry, K. J. Somaiya College of Science & Commerce, Mumbai – 400 077, Maharashtra, India. http://orcid.org/0000-0003-0854-0602
  • Sasangan Kc Department of Biochemistry, K. J. Somaiya College of Science & Commerce, Mumbai – 400 077, Maharashtra, India.

Abstract

Objective: In this study, the leaf powder of Aegle marmelos (L.) Correa. was subjected to preliminary phytochemical and in vitro antioxidant analysis.

Methods: The freshly prepared plant leaf extract was subjected to preliminary phytochemical screening, which revealed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic compounds, terpenoids, and steroids. Reducing power, superoxide (SO) anion radical, nitric oxide (NO) radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the methanolic leaf extract of this plant. The amounts of total phenolic and flavonoid compounds were also determined.

Results: This study has revealed that the A. marmelos (L.) Correa. leaf extract showed considerably high amounts of most of the phytochemicals, total antioxidant capacity, total phenolic, and flavonoid content. The study also indicated that the A. marmelos (L.) Correa. showed comparatively good scavenging activity, i.e., inhibition of hydroxyl radical, NO and SO anion scavenging and reducing power activities when compared with the respective standards.

Conclusion: The leaf powder of A. marmelos (L.) Correa. can be used as easily accessible source of natural antioxidant and as a possible food supplement or in pharmaceutical industry.

Keywords: Aegle marmelos (L.) Correa., Preliminary phytochemical analysis, In vitro-antioxidant activity.

Author Biography

Samidha M Pawaskar, Department of Biochemistry, K. J. Somaiya College of Science & Commerce, Mumbai – 400 077, Maharashtra, India.

Dr. S. M. Pawaskar,

Dean, Research and Extension,

Head, Department of Biochemistry,

K. J. Somaiya College of Science and Commerce,

Vidyavihar, Mumbai 400 077

References

1. Panovska TK, Kulevanova S, Stefova M. In vitro antioxidant activity of some Teucrium species (Lamiaceae). Acta Pharm 2005;55(2):207-14.
2. Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food Chem Toxicol 2006;44(2):198-206.
3. Patel VR, Patel P, Kajal SS. Antioxidant activity of some selected medicinal plants in Western region of India. Adv Biol Res 2010;4(1):23-6.
4. Modi H, Patel V, Patel K. Hepatoprotective activity of Aegle marrmelos against ethanol induced hepatotoxicity in rats. Asian J Pharm Clin Res 2012;5(4):164-7.
5. Pawaskar SM, Kale KU. Evaluation of phytochemical and anti-oxidative potential of aqueous whole plant extract of Mimosa pudica. Indian J Clin Biochem 2007;22.
6. Rajeshwari S, Balakrishnan A, Thenmozhi M, Venckatesh R. Preliminary phytochemical analysis of Aegle marmelos, Ruta graveolens, Opuntia dellini, Euphorbia royleana and Euphorbia antiquorum. Int J Pharm Sci Res 2011;2(1):146-50.
7. Trease GE, Evans WC. Pharmacognsy. 11th ed. London: Brailliar Tiridel Can, Macmillan Publishers; 1989.
8. Oguyemi AO. In: Sofowora A. editor. Proceedings of a Conference on African Medicinal Plants. Ife: University Ife; 1979. p. 20-2.
9. Venkatesan D, Karrunakarn CM, Kumar SS, Swamy PT. Identification of phytochemical constituents of Aegle marmelos responsible for antimicrobial activity against selected pathogenic organisms. Ethnobot Lealf 2009;13:1362-72.
10. Finar LL. Organic Chemistry. 25th ed. London: Longman; 1983. p. 696-765.
11. Martinez A, Valencia G. Manual de practicas de Farmacognosia y Fitoquimia: 1999.1. Medellin: Universidad de Antiquia; Marcha fotiquimica; 2003. p. 59-65.
12. Harbone JB. Phytochemical Methods. 1st ed. London, UK: Champan and Hall; 1973.
13. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.
14. McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.
15. Oyaizu M. Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine.Jap J Nutr 1986;44:307-15.
16. Athukorala Y, Kim KN, Jeon YJ. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol 2006;44(7):1065-74.
17. Kunchandi E, Rao MN. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237-40.
18. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JK, Tannenbaum SR. Analysis of nitrate, nitrite and 15 N in biological fluids. Anal Biochem 1982;126:131-6.
19. Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochem Biophys Res Commun 1994;201(2):748-55.
20. Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 1988;37(5):837-41.
21. Ulahannan RK, Thomas T, Sadasivan C. Antibacterial action of leaves of Aegle marmelos. Int J Sci 2008;2(2):134-8.
22. Siddique NA, Mujeeb M, Ansari SH, Ahmad S, Ali B, Aslam J. Development of quality standards of Aegle marmelos L. leaves. J Phytol 2010;2(2):36-43.
23. Mier S, Kaner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 1995;43:1813-7.
24. Ram RP, Mehrotra NB. Compendium of India Medicinal Plants. New Delhi: CDRI, NISC; 1996. p. 371.
25. Korkina LG, Afanas’ev IB. Antioxidant and chelating properties of flavonoids. Adv Pharmacol 1997;38:151-63.
26. Vanitha V, Umadevi KJ, Vijayalakshmi K. In vitro assessment of alcoholic leaf extracts of Annona squamosa and Aegle marmelos. Bioscan 2010;5(2):225-9.
27. Khanam S, Shivprasad HN, Kshama D. In vitro antioxidant screening models: A review. Indian J Pharm Educ 2004;38:180.
28. Bora KS, Sharma A. In vitro antioxidant and free radical scavenging potential of Medicago sativa Linn. J Pharm Res 2010;3:1206-10.
29. Vidhya N, Devaraj SN. Antioxidant effect of eugenol in rat intestine. Indian J Exp Biol 1999;37(12):1192-5.
Statistics
448 Views | 69 Downloads
Citatons
How to Cite
Pawaskar, S. M., and S. Kc. “IN VITRO - ANTIOXIDANT AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF AEGLE MARMELOS (L.) CORREA. LEAF EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 6, June 2017, pp. 367-72, doi:10.22159/ajpcr.2017.v10i6.17808.
Section
Original Article(s)