POLYMORPHISM OF CYTOCHROME P450, SUPERFAMILY19, POLYPEPTIDE 1 GENE AND RELATED TO AROMATASE ENZYME ACTIVITY IN ACROMEGALY IRAQI PATIENTS


Iqbal Hanash Dhefer, Salma Abdul Rudha Abbas, Najwa Sh Ahmed

Abstract


Objective: The objective of the present work was to investigate the association between aromatase enzyme activity and polymorphisms, rs2236722, in exon 2 of cytochrome P450 (CYP), superfamily19, polypeptide 1 (CYP19A1gene) in patients with active acromegaly.

Methods: A total of 120 males and females (age 20-60 years) were enrolled in this study, 60 patients with active acromegaly who have attended the National Diabetes Centre and and Specialist Center for endocrinology and diabetes, Baghdad, from December 2015 to June 2016 and 60 healthy individuals with matches as a control group. From the whole blood, genomic DNA was extracted to perform genotyping analysis and single-nucleotide polymorphism, rs2236722, in the CYP19A1 gene using multiplex polymerase chain reaction technique, and serum aromatase levels were determined using a solid-phase enzyme-linked immunosorbent assay based on sandwich method.

Results: The results show that the aromatase activity levels of patients were a highly significant decrease when contrasted with healthy control groups in both sexes (p≤0.01), and there were summarized positive results of an allele in single-nucleotide polymorphism, rs2236722, were highly significant in TT allele when contrasted with healthy control groups. While revealed that there was non-significant difference in TC allele when contrasted with healthy control groups (p>0.01).

Conclusion: we conclude that there was associated between CYP19A1 gene polymorphism (rs2236722) with aromatase activity and related to acromegaly patients.


Keywords


Aromatase activity, Single-nucleotide polymorphism, rs2236722, Active acromegaly.

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About this article

Title

POLYMORPHISM OF CYTOCHROME P450, SUPERFAMILY19, POLYPEPTIDE 1 GENE AND RELATED TO AROMATASE ENZYME ACTIVITY IN ACROMEGALY IRAQI PATIENTS

Keywords

Aromatase activity, Single-nucleotide polymorphism, rs2236722, Active acromegaly.

DOI

10.22159/ajpcr.2017.v10i6.17886

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 228-232

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

50 Views | 50 Downloads

Authors & Affiliations

Iqbal Hanash Dhefer
Department of Chemistry, The University of Al-Mustansiriya, College of Science, Baghdad, Iraq
Iraq

Salma Abdul Rudha Abbas
Department of Chemistry, The University of Al-Mustansiriya, College of Science, Baghdad, Iraq
Iraq

Najwa Sh Ahmed
Department of Biotechnology, Biotechnology Research, Molecular, and Biotechnology Laboratory, AL-Nahrain University, Baghdad, Iraq
Iraq


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