IN VITRO ANTIBACTERIAL ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM ETHYL ACETATE EXTRACT OF HYDROCOTYLE SIBTHORPIODES AGAINST MULTI DRUG RESISTANT MICROBES.


Manashi Garg, Banasmita Devi, Rashna Devi

Abstract


 Objectives: Hydrocotyle sibthorpiodes is known to contain several phytoconstituents which are constantly involved in the formation of Silver nanoparticles that may affect several multi-drug resistant microbes. Therefore, the study was undertaken to evaluate the efficacy of different concentration of nano silver solution on three bacterial isolates. It was also aimed to qualitatively assess the different phytoconstituents responsible for the synthesis.

Methods: Three bacterial isolates of Klebsiella pneumonia, Pseudomonas aeroginosa and Staphylococcus aureus were identified. Synthesis of AgNPs with different concentration (2/4/6/8/10μl/ml) was done and applied to the selected isolates. The phytochemical compounds of the ethyl acetate extract were assayed by several colored reactions qualitatively.

Results: The size and stability biosynthesis of the metallic silver nanoparticles were confirmed by photophysical characterization as well as SEM (Scanning Electron Microscopy), XRD (XRay Diffraction), Zeta potential and DLS (Dynamic Light Scattering) with an average size of 13.37 ±10 nm. The increasing concentration of the particle solution showed significant inhibition zone for all the three isolates viz., Klebsiella pneumonia, Pseudomonas aeroginosa and Staphylococcus. aureus showing the value of 3.0±0.17, 2.7±0.32 and 3.6±0.57 respectively for 10 μl/ml concentration. Phytochemical screening of the whole plant extract also revealed an array of bioactive compounds which may have an effective role in the reduction process.

Conclusion: The study demonstrated a simple, efficient and eco-friendly synthesis of stable silver nanoparticles from the ethyl acetate extract of Hydrocotyle sibthorpiodes having fairly superior antimicrobial activity against human pathogens.


Keywords


Multidrug resistant, Hydrocotyle sibthorpioides, Antibacterial activity, Silver nanoparticles, Qualitative assay.

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References


Mohanpuria P, Yadav PN, Yadav SK. Biosynthesis of nanoparticle, technological concepts and future application. J Nanopart 2007;7:9275.

Khoo KS, Gwee PS, Beh V, Chung TC, Tan LY, Sit NW, et al. Antifungal and antibacteral properties of three medicinal plants from Malaysia. Acta Hortic 2016;15:1023.

Rai SK. Medicinal plants used by Meche people of Jhapa district, Eastern Nepal. Our Nat 2004;2:27-32.

Yu F, Yu F, McGuire PM, Li R, Wang R. Effects of Hydrocotyle sibthorpioides extract on transplanted tumors and immune function in mice. Phytomedicine 2007;14(2-3):166-71.

Rajesh S, Patric DR, Rathi JM, Sahayaraj K. Biosynthesis of silver nanoparticles using Ulva fasciata (Delile) ethyl acetatextract and its activity against Xanthomonas campestris pv. Malvacearum. J Biopesticide 2012:119-28.

Schaad NW, Jones JB, Chun W. The bacteriology committee of the American phyto pathological society. Plant Pathogenic Bacteria. 3rd ed. USA: APS Press; 2001.

Sarma G, Nakuleswar JD, Prerna S, Suresh JC. Synthesis, characterization and antimicrobial activity of Belia grandiflora assisted AgNPs. J Microbiol Biochem Technol 2014;6:274-8.

Singh K, Manju P, Sangeeta K, Uma C, Prakash YJ. Antibacterial activity of synthesized silver nanoparticles from Tinospora cordofolia against multi drug resistant strains of Pseudomonas aeroginosa isolated from burn patients. J Nanomed Nanotechnol 2014;5:192.

Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine 2007;3(1):95-101.

Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 2000;52(4):662-8.

Aswathy Aromal S, Philip D. Green synthesis of gold nanoparticles using Trigonella foenum-graecum and its size-dependent catalytic activity. Spectrochim Acta A Mol Biomol Spectrosc 2012;97:1-5.

Hussain JI, Kumar S, Hashmi AA, Khan Z. Silver nanoparticles: Preparation, characterization and kinetics. Adv Mater 2011;2(3):188-94.

Kumar GG, Sangita MS, Bappadity M, Kiran M. Phytochemical evaluation of methanolic extract of Zizyphus xylopyrus. Int J Drug Discov 2011;1:231-3.

Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K. Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 2011;79(3):594-8.

Dubey SP, Lahtinen M, Sillanpaa M. Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugos. Process Biochem 2010;45:1065-71.

Othman AS, Ahmed NA. Antibacteral effect of the ethanol leaves extract of Moringa oleifera and Camellia sinesis against multi drug resistant bacteria. Int J Pharmacol 2016;13(2):156-65.

Khalil MM, Ismaila EH, El-Baghdadyc KZ, Mohamed D. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian J Chem 2014;7(6):1131-9.

Chen D, Qiao X, Qiu X, Chen J. Synthesis and electrical properties of uniform silver nanoparticles for electronic applications. J Mater Sci 2009;44(4):1076-81.

Jiale H, Guowu Z, Bingyun Z, Daohua S, Fenfen L, Yuan L, et al. Biogenic silver nanoparticles by Cacumen platycladi extract: Synthesis, formation mechanism, and antibacterial activity. Ind Eng Chem Res 2009;50:9095-106.

Zayed MF, Eisa WH, Shabaka AA. Malva parviflora extract assisted green synthesis of silver nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc 2012;98:423-8.

Patil RS, Kokate MR, Kolekar SS. Bioinspired synthesis of highly stabilized silver nanoparticles using Ocimum tenuiflorum leaf extract and their antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 2012;91:234-8.

Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 2004;275:496-502.

Amendola V, Bakr OM, Stellacci F. A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: Effect of shape, size, structure assembly. Plasmonics 2010;5(1):85-97.

Banothu V, Adepally U, Lingam J. In vitro total phenolics, flavonoids contents, antioxidant and antimicrobial activities of various solvent extracts from the medicinal plant Physalis minima. Int J Pharm Pharm Sci 2017;9(3):192-8.

Hendra P, Andreani PR, Pangestuti E, Julianus J. Evaluation of antihyperlipidemic, anti-inflammatory and analgesics activities of Eurycoma longifolia in animal models. Int J Pharm Pharm Sci 2017;9(3):166-9.

Castañón GA, Martínez F, Gutierrez J, Facundo R. Synthesis and antibacterial activity of silver nanoparticles with different sizes. J Nanopart Res 2008;10:1343-8.

Ren Y, Hui Y, Wang T, Wang C. Green synthesis and antimicrobial activity of monodisperse silver nanoparticles synthesized using Ginkgo biloba leaf extract. Phys Lett A 2016;380(45):3773-7.

Paredes D, Ortiz C, Torres, R. Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA). Int J Nanomed 2014;9:1717-29.

Nasim K, Asghar T. Antibacterial effect of silver nanoparticles on Acinetobacter baumannii. Iran J Public Health 2014;43:39.

Singh K, Panghal M, Kadyan S, Chaudhary U, Yadav JP. Antibacterial activity of synthesized silver nanoparticles from Tinospora cordifolia against multi drug resistant strains of Pseudomonas aeruginosa isolated from burn patients. J Nanomed Nanotechnol 2014;5:2.




About this article

Title

IN VITRO ANTIBACTERIAL ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM ETHYL ACETATE EXTRACT OF HYDROCOTYLE SIBTHORPIODES AGAINST MULTI DRUG RESISTANT MICROBES.

Keywords

Multidrug resistant, Hydrocotyle sibthorpioides, Antibacterial activity, Silver nanoparticles, Qualitative assay.

DOI

10.22159/ajpcr.2017.v10i6.17976

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 263-266

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

71 Views | 70 Downloads

Authors & Affiliations

Manashi Garg
1Department of Biotechonology, Assam downtown University, Panikhaiti, Guwahati, Assam, India.
India

Banasmita Devi
Department of Biochemistry, Assam downtown University, Panikhaiti, Guwahati, Assam, India.
India

Rashna Devi
Department of Biochemistry, Assam downtown University, Panikhaiti, Guwahati, Assam, India.
India


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