UROPATHOGENS: ISOLATION AND ANTIBACTERIAL SUSCEPTIBILITY PATTERN
Objective: To find the antibiotic sensitivity pattern of uropathogens.
Methods: A total of 100 urine samples of clinically suspected urinary tract infection were collected from May 2016 to July 2016. The samples were inoculated on cystine lactose electrolyte deficient agar and incubated at 37Â°C for 24 hrs.
Results: A total of 77 (77%) samples were positive showing microbial growth. Among all isolates, Escherichia coli was 36.36%, followed by Klebsiella spp. (35.06%), Citrobacter spp. (6.49%), Staphylococcus aureus (6.49%), Pseudomonas spp. (5.19%), Enterococcus faecalis (3.90%), and Acinetobacter spp. (1.30%). For Gram-positive isolates, the most effective antibiotic was nitrofurantoin followed by sulphafurazole. For Gram-negative isolates, the most effective antibiotic was nitrofurantoin followed by gentamicin and piperacillin/tazobactam.
Conclusion: In this study, nitrofurantoin was the most effective antibiotic for Gram-positive and Gram-negative uropathogens
2. Pezzlo M. Detection of urinary tract infections by rapid methods. Clin Microbiol Rev 1988;1(3):268-80.
3. Acharya VN. Urinary tract infection â€“ A dangerous and unrecognised forerunner of systemic sepsis. J Postgrad Med 1992;38(2):52-4.
4. Maji SK, Mandal PK, Panja C, Dolai TC, Samanta A, Kundu PK, et al. Prevalence and antibacterial susceptibility pattern of aerobic bacteria causing urinary tract infection in tribal population in rural part of West Bengal, India. Int J Curr Microbiol App Sci 2016;5(6):406-12.
5. Foxman B. Epidemiology of urinary tract infections: Incidence, morbidity, and economic costs. Dis Mon 2003;49(2):53-70.
6. Muraleetharan M, Viswanathan T. Epidemiological studies on varying extended-spectrum Î²-lactamases producing uropathogenic bacteria. Int J Pharm Pharm Sci 2014;6(11):57-60.
7. Mollick S, Dasgupta T, Hasnain MJ, Ahmed M. Isolation and characterization of pathogens responsible for urinary tract infection in Bangladesh and determination of their antibiotic susceptibility pattern. J App Pharm Sci 2016;6(04):72-6.
8. Chaudhary BL, Insan NG, Payal N, Vashistha R, Rajput A. ESBL and AmpC detection in Klebsiella species by non molecular methods. Int J Curr Microbiol App Sci 2014;3(4):323-9.
9. El Astal Z. Increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in Gaza Strip, Palestine. J Biomed Biotechnol 2005;2005(3):238-41.
10. Mahesh E, Ramesh D, Indumathi VA, Punith K, Raj K, Anupama HA. Complicated urinary tract infection in a tertiary care center in south India. Al Ameen J Med Sci 2010;3(2):120-7.
11. Basavaraj MB, Jyothi P. Antibiotic sensitivity pattern of Citrobacter spp. Isolated from patients with urinary tract infections in tertiary care hospital in south India. Int J Pharm Pharm Sci 2015;7(1):252-4.
12. Kothari A, Sagar V. Antibiotic resistance in pathogens causing community acquired urinary tract infections in India: A multicentric study. J Infect Dev Ctries 2008;2:354-8.
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