UTILITY OF ANTIGEN DETECTION TEST AND POLYMERASE CHAIN REACTION IN THE DIFFERENTIATION OF TUBERCULOUS AND NON-TUBERCULOUS MYCOBACTERIA


Yogita Singh, Raji Vasanth, Shrikala Baliga, Dhanashree B

Abstract


Objectives: Cultivation and identification of mycobacteria to species level remains difficult and time-consuming. Hence, easy and rapid diagnostic methods are necessary for the differentiation of Mycobacterium tuberculosis (MTB) from non-tuberculous mycobacteria (NTM). The present study aims to detect and differentiate MTB from NTM isolated from clinical samples by immunochromatographic test (ICT) and polymerase chain reaction (PCR).

Methods: Over a period of 1 year, clinical samples (n=496) received from suspected cases of TB, at the Department of Microbiology, Kasturba Medical College Hospital, Mangalore were cultured to isolate Mycobacterium spp. Identification of all the isolates was done by conventional biochemical technique, ICT, and PCR.

Results: Among the 496 samples processed, 49 (9.87%) were acid-fast bacilli smear positive and 59 (11.89%) samples showed the growth of Mycobacterium spp. Among these, 10 were rapid growers, 49 were slow-growing mycobacteria, out of which 30 were MTB as identified by conventional biochemical reaction. Out of 59 Mycobacterial isolates subjected to ICT for the detection of MPT 64 antigen, only 28 were identified as MTB. However, all the 30 isolates were correctly identified as MTB by PCR.

Conclusion: Hence, PCR is essential for rapid differentiation of non-tuberculous Mycobacterium from MTB. False negative results seen with immunochromatographic MPT 64 antigen assay could be due to mutations within the mpt64 gene. Further studies are necessary to characterize these PCR-positive and immunochromatographic assay negative MTB isolates.


Keywords


Mycobacterium tuberculosis, Polymerase chain reaction, Culture, Antigen detection.

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About this article

Title

UTILITY OF ANTIGEN DETECTION TEST AND POLYMERASE CHAIN REACTION IN THE DIFFERENTIATION OF TUBERCULOUS AND NON-TUBERCULOUS MYCOBACTERIA

Keywords

Mycobacterium tuberculosis, Polymerase chain reaction, Culture, Antigen detection.

DOI

10.22159/ajpcr.2017.v10i6.18261

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 289-291

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

62 Views | 58 Downloads

Authors & Affiliations

Yogita Singh
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
India

Raji Vasanth
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
India

Shrikala Baliga
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
India

Dhanashree B
Associate Professor, Department of Microbiology, Kasturba Medical College, Mangalore Manipal University
India


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