EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY AND ESTIMATION OF TOTAL PHENOL AND FLAVONOID CONTENT OF ETHANOLIC LEAF EXTRACT OF IRIS KASHMIRIANA
Objective: The main aim of this study was to determine the in vitro antioxidant activity of Iris kashmiriana ethanolic leaf extract and also total phenol and flavonoid content was evaluated.
Methods: Total phenol content (TPC) was determined by Folinâ€“Ciocalteu method, total flavonoid content (TFC) was estimated by aluminum trichloride spectrophotometer method. Furthermore, antioxidant activity was revealed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) scavenging activity, and reducing power assay.
Results: The ethanolic leaf extract of I. kashmiriana showed TPC of 13.25Â±0.57 Î¼g/100 Î¼g gallic acid equivalents and TFC of 33.61Â±3.37 Î¼g/100 Î¼g rutin equivalents. The DPPH assay revealed IC50 of 0.418 mg/ml and for H2O2 radical scavenging IC50 was 0.476 mg/ml for the plant extract while as reducing power assay revealed concentration-dependent absorption values which clearly determine the antioxidant property of plant.
Conclusion: From the results, it is apparent that I. kashmiriana ethanolic leaf extract possessed potential antioxidant activity which can be used to cure wide range of diseases.
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