• Shah Khalid Department of Botany, Pest Control and Ayurvedic Drug Research Lab, Vidisha, Madhya Pradesh, India.
  • Aparna Alia Department of Botany, Rajeev Gandhi College, Shahpura, Bhopal, Madhya Pradesh, India.
  • Shrivastava Pn Department of Botany, Pest Control and Ayurvedic Drug Research Lab, Vidisha, Madhya Pradesh, India.
  • Muzafar Akbar Rather Department of Botany, Pest Control and Ayurvedic Drug Research Lab, Vidisha, Madhya Pradesh, India.
  • Muzafar Ah Sheikh Department of Zoology, Government Degree College, Ganderbal, Jammu and Kashmir, India.


Objective: The main aim of this study was to determine the in vitro antioxidant activity of Iris kashmiriana ethanolic leaf extract and also total phenol and flavonoid content was evaluated.

Methods: Total phenol content (TPC) was determined by Folin–Ciocalteu method, total flavonoid content (TFC) was estimated by aluminum trichloride spectrophotometer method. Furthermore, antioxidant activity was revealed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) scavenging activity, and reducing power assay.

Results: The ethanolic leaf extract of I. kashmiriana showed TPC of 13.25±0.57 μg/100 μg gallic acid equivalents and TFC of 33.61±3.37 μg/100 μg rutin equivalents. The DPPH assay revealed IC50 of 0.418 mg/ml and for H2O2 radical scavenging IC50 was 0.476 mg/ml for the plant extract while as reducing power assay revealed concentration-dependent absorption values which clearly determine the antioxidant property of plant.

Conclusion: From the results, it is apparent that I. kashmiriana ethanolic leaf extract possessed potential antioxidant activity which can be used to cure wide range of diseases.

Keywords: Iris kashmiriana, Total phenol content, Total flavonoid content, 1, 1-diphenyl-2-picrylhydrazyl, Hydrogen peroxide.

Author Biography

Shah Khalid, Department of Botany, Pest Control and Ayurvedic Drug Research Lab, Vidisha, Madhya Pradesh, India.

Research Scholar

Department Of Botany

Pest Control and Ayurvedic Drug Research Lab, Vidisha, M.P. India 464001.


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How to Cite
Khalid, S., A. Alia, S. Pn, M. A. Rather, and M. A. Sheikh. “EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY AND ESTIMATION OF TOTAL PHENOL AND FLAVONOID CONTENT OF ETHANOLIC LEAF EXTRACT OF IRIS KASHMIRIANA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 8, Aug. 2017, pp. 309-12, doi:10.22159/ajpcr.2017.v10i8.18268.
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