EXTRACTION, ISOLATION, AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS AND ESSENTIAL OIL FROM SYZYGIUM JAMBOS


Mosad Ahmed Ghareeb, Manal Mortady Hamed, Abdel-aleem Hassan Abdel-aleem, Amal Mohamed Saad, Mohamed Sayed Abdel-aziz, Asmaa Hadad

Abstract


Objectives: Over the past few decades, phenolic compounds become important due to it has been associated with protection against different diseasesand sensory point of vision. Hence, at the present study, there has been a growing interest to carry out structural elucidation and characterization ofthe pure isolates from Syzygium jambos.

Methods: S. jambos dried powder leaves were extracted by soaking in 85% methanol solvent at room temperature 25±2°C. The antioxidative activityof the isolates was assessed according to 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum assays.

Results: A total of 8 compounds were isolated from the n-butanol extract of S. jambos (Family Myrtaceae) plant, they were identified as quercetin-3-O-rutinoside (1), prenylbenzoic acid 4-β-D-glucoside (2), morolic acid 3-O-caffeate (3), 5,4’-dihydroxy, 7-methoxy, 6-methyl-flavone (4),3,4,5-trihydroxybenzoic acid (5), quercetin (6), isoetin-7-O-β-D-glucopyranoside (7), and (4’-hydroxy-3’-methoxyphenol-β-D-[6-O-(4”-hydroxy-3”,5”-dimethoxylbenzoate)] glucopyranoside) (8). Compounds 5 and 6 showed the most radical scavengers among the tested compounds with SC50 values of5.50 and 4.30 μg/ml, respectively, compared to ascorbic acid as standard and the total antioxidant capacity (TAC) values of 605.0 and 680.59 mg ascorbicacid equivalent/g compound, respectively. In vitro antimicrobial activities of the isolated compounds were tested using disc agar plate method againstfour pathogenic microbial strains including Gram-positive, Gram-negative bacteria and yeast with inhibition zones from 9 to 19 mm. Gas chromatographymassspectrometry analysis for the essential oil provides twenty four identified components representing 92% of its total oil composition.

Conclusion: The results supported that S. jambos could be attributed to sources of natural antioxidant and antimicrobial applications.


Keywords


Syzygium jambos, Antioxidant compounds, Flavonoids, Natural products, Phenols

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About this article

Title

EXTRACTION, ISOLATION, AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS AND ESSENTIAL OIL FROM SYZYGIUM JAMBOS

Keywords

Syzygium jambos, Antioxidant compounds, Flavonoids, Natural products, Phenols

DOI

10.22159/ajpcr.2017.v10i8.18849

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 8 August 2017 Page: 194-200

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

242 Views | 50 Downloads

Authors & Affiliations

Mosad Ahmed Ghareeb
Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt. 2Department of Organic Chemistry, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt
Egypt

Manal Mortady Hamed
Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt. 2Department of Organic Chemistry, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt
Egypt

Abdel-aleem Hassan Abdel-aleem
Department of Organic Chemistry, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt
Egypt

Amal Mohamed Saad
Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt.
Egypt

Mohamed Sayed Abdel-aziz
Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Giza, Egypt
Egypt

Asmaa Hadad
Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt.
Egypt


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