• Ahmadi Yusmin Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410,Brunei Darussalam
  • Norhayati Ahmad Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410, Brunei Darussalam http://orcid.org/0000-0002-3904-5981



 Objective: Nigella sativa and its active constituent thymoquinone (TQ) have been extensively documented for its pharmacological values, but its application in wound healing in particular in a diabetic wound healing model is less documented.

Methods: In our study, alloxan-induced diabetic rats were used as a chronic delayed wound model and topical administration of TQ 10% w/v were used to assess the role and function of TQ in wound healing through wound contraction and histological analysis.

Results: Although statistically insignificant, we found out that TQ accelerated wound healing in post-wounding day 3 (inflammatory phase), whereas aggressively decelerating wound healing in post-wounding day 7 (proliferation phase). In addition, our histological analyses of wound granulation tissues at post-wounding day 14 substantiate our claim by showing that TQ treatment had delayed wound healing progression of the diabetic rats.

Conclusions: Our study shows that TQ accelerates wound healing during the inflammatory phase; however, decelerate rapidly during the proliferation phase. We speculate the acceleration of wound healing during the inflammatory phase was due to its well-documented antioxidant, anti-inflammatory, and antimicrobial properties while its deceleration of wound healing during the proliferation phase was due to its well-documented antiangiogenic effect.

Keywords: Wound healing, Thymoquinone, Nigella sativa, Diabetes mellitus.

Author Biographies

Ahmadi Yusmin, Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410,Brunei Darussalam
Graduate student, Environmental & Life Sciences, Faculty of Science
Norhayati Ahmad, Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong BE1410, Brunei Darussalam


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How to Cite
Yusmin, A., and N. Ahmad. “EFFECT OF THYMOQUINONE ON WOUND HEALING IN ALLOXAN-INDUCED DIABETIC RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 9, Sept. 2017, pp. 242-5, doi:10.22159/ajpcr.2017.v10i9.18951.
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